John Giannini
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View article: Artificial intelligence assisted clinical fluorescence imaging achieves in vivo cellular resolution comparable to adaptive optics ophthalmoscopy
Artificial intelligence assisted clinical fluorescence imaging achieves in vivo cellular resolution comparable to adaptive optics ophthalmoscopy Open
Background Advancements in biomedical optical imaging have enabled researchers to achieve cellular-level imaging in the living human body. However, research-grade technology is not always widely available in routine clinical practice. In t…
View article: Assessing the Data Quality Dimensions of Partial and Complete Mastectomy Cohorts in the <i>All of Us</i> Research Program: Cross-Sectional Study
Assessing the Data Quality Dimensions of Partial and Complete Mastectomy Cohorts in the <i>All of Us</i> Research Program: Cross-Sectional Study Open
Background Breast cancer is prevalent among females in the United States. Nonmetastatic disease is treated by partial or complete mastectomy procedures. However, the rates of those procedures vary across practices. Generating real-world ev…
View article: Assessing the Data Quality Dimensions of Partial and Complete Mastectomy Cohorts in the All of Us Research Program: A Cross-Sectional Study (Preprint)
Assessing the Data Quality Dimensions of Partial and Complete Mastectomy Cohorts in the All of Us Research Program: A Cross-Sectional Study (Preprint) Open
BACKGROUND Breast cancer is prevalent among women in the United States. Non-metastatic disease is treated by partial or complete mastectomy procedures. However, the rates of those procedures vary across practices. Generating real-world ev…
View article: Bacterial Meningitis With Cerebral Edema in a Young Adult: A Simulation Case for Medical Students
Bacterial Meningitis With Cerebral Edema in a Young Adult: A Simulation Case for Medical Students Open
Medical simulation is a valuable educational tool tailored to maximize student learning and supplement the traditional didactic curriculum. The successful development and implementation of our meningitis simulation case further supports th…
View article: Opportunities for Ophthalmology Research in Large Research Cohorts: A National Eye Institute, National Institute of Biomedical Imaging and Bioengineering, and All of Us Research Program Workshop
Opportunities for Ophthalmology Research in Large Research Cohorts: A National Eye Institute, National Institute of Biomedical Imaging and Bioengineering, and All of Us Research Program Workshop Open
The potential inclusion of ocular imaging data into the All of Us Research Program (All of Us) dataset provides a unique opportunity to address many of these issues and empower new opportunities for research. To discuss the opportunities a…
View article: Three-dimensional structured illumination microscopy with enhanced axial resolution
Three-dimensional structured illumination microscopy with enhanced axial resolution Open
We present two distinct, complementary methods for improving axial resolution in three-dimensional structured illumination microscopy (3D SIM) with minimal or no modification to the optical system. First, we show that placing a mirror dire…
View article: Visualizing retinal cells with adaptive optics imaging modalities using a translational imaging framework
Visualizing retinal cells with adaptive optics imaging modalities using a translational imaging framework Open
Adaptive optics reflectance-based retinal imaging has proved a valuable tool for the noninvasive visualization of cells in the living human retina. Many subcellular features that remain at or below the resolution limit of current in vivo t…
View article: In-vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection
In-vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection Open
Adaptive optics scanning light ophthalmoscopy (AOSLO) allows non-invasive visualization of the living human eye at the microscopic scale; but even with correction of the ocular wavefront aberrations over a large pupil, the smallest cells i…
View article: Integrating adaptive optics-SLO and OCT for multimodal visualization of the human retinal pigment epithelial mosaic
Integrating adaptive optics-SLO and OCT for multimodal visualization of the human retinal pigment epithelial mosaic Open
In vivo imaging of human retinal pigment epithelial (RPE) cells has been demonstrated through multiple adaptive optics (AO)-based modalities. However, whether consistent and complete information regarding the cellular structure of the RPE …
View article: Supplementary document for In vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection - 5076584.pdf
Supplementary document for In vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection - 5076584.pdf Open
Supplemental Document
View article: Medical Simulation-Based Learning Outcomes in Pre-Clinical Medical Education
Medical Simulation-Based Learning Outcomes in Pre-Clinical Medical Education Open
Introduction Medical simulation is widely used in the United States medical curriculum. However, learning outcomes based on simulation have yet to be reported. In this study, we aim to characterize the objective performance of first- and s…
View article: Anticipating, measuring, and minimizing MEMS mirror scan error to improve laser scanning microscopy's speed and accuracy
Anticipating, measuring, and minimizing MEMS mirror scan error to improve laser scanning microscopy's speed and accuracy Open
We describe a method to speed up microelectromechanical system (MEMS) mirror scanning by > 20x, while also improving scan accuracy. We use Landweber deconvolution to determine an input voltage which would produce a desired output, based on…
View article: Single-shot super-resolution total internal reflection fluorescence microscopy
Single-shot super-resolution total internal reflection fluorescence microscopy Open
We demonstrate a simple method for combining instant structured illumination microscopy (SIM) with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF (down to 115 +/-13 nm) and enabling imagin…
View article: Fig_3_Data_for_naive
Fig_3_Data_for_naive Open
Data for Fig. 3. Contains the desired result, measured result, and masked and full residuals for scan patterns of three different lengths.
View article: S1_Fig_Data
S1_Fig_Data Open
Data for S1 Fig. Contains 3 input voltages in CSV form. Input voltages were generated using the algorithm described in the paper.
View article: Fig_7_Data_for_full_method
Fig_7_Data_for_full_method Open
Data for Fig. 7. Contains a series of optimization results, including the desired result, achieved result, masked and unmasked residual, and voltage mask for 5 iterations.
View article: Fig_5_Data_for_linear_decon
Fig_5_Data_for_linear_decon Open
Data for Fig. 5. Contains the optimization results, including the desired result, achieved result, and masked and unmasked residual for 1 iterations. Also includes data from Fig. 3 for comparison.
View article: S2_Fig_Data
S2_Fig_Data Open
Data for S2 Fig. Contains desired and achieved results in CSV form for both masked and unmasked optimization.
View article: Fig_6_Data_for_overview_schematic
Fig_6_Data_for_overview_schematic Open
Data for Fig. 6. Contains a series of optimization results, including the desired result, achieved result, and masked residual for 7 iterations. Also includes the mirror impulse response.