John K. Raison
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View article: Sensitivity of Altitudinal Ecotypes of the Wild Tomato <i>Lycopersicon hirsutum</i> to Chilling Injury
Sensitivity of Altitudinal Ecotypes of the Wild Tomato <i>Lycopersicon hirsutum</i> to Chilling Injury Open
The transition temperature of the leaf polar lipids and the critical temperature for chill-induced inhibition of photosynthesis was determined for three altitudinal ecotypes of the wild tomato Lycopersicon hirsutum. Photosynthesis was meas…
View article: Compositional and Thermal Properties of Thylakoid Polar Lipids of <i>Nerium oleander</i> L. in Relation to Chilling Sensitivity
Compositional and Thermal Properties of Thylakoid Polar Lipids of <i>Nerium oleander</i> L. in Relation to Chilling Sensitivity Open
The polar lipid classes from thylakoids of Nerium oleander L. were studied with the aim of relating changes in their composition and thermal behavior with reported changes in the transition temperature of their polar lipids and chilling se…
View article: Phase Transitions in Liposomes Formed from the Polar Lipids of Mitochondria from Chilling-Sensitive Plants
Phase Transitions in Liposomes Formed from the Polar Lipids of Mitochondria from Chilling-Sensitive Plants Open
The thermal response of mitochondrial polar lipids from a variety of chilling-sensitive and chilling-insensitive plants was determined by differential scanning calorimetry. A phase transition was observed at 15 degrees C for mitochondria f…
View article: Phase Transitions in Thylakoid Polar Lipids of Chilling-Sensitive Plants
Phase Transitions in Thylakoid Polar Lipids of Chilling-Sensitive Plants Open
The phase behavior of thylakoid polar lipids from plants sensitive to chilling injury was investigated by calorimetry, electron spin resonance spectroscopy of spin labels, and fluorescence intensity after labeling with trans-parinaric acid…
View article: Growth Temperature-Induced Alterations in the Thermotropic Properties of <i>Nerium oleander</i> Membrane Lipids
Growth Temperature-Induced Alterations in the Thermotropic Properties of <i>Nerium oleander</i> Membrane Lipids Open
The temperature boundary for phase separation of membrane lipids extracted from Nerium oleander leaves was determined by analysis of spin label motion using electron spin resonance spectroscopy and by analysis of polarization of fluorescen…
View article: Thermal Behavior and Lipid Composition of Cauliflower Plasma Membranes in Relation to ATPase Activity and Chilling Sensitivity
Thermal Behavior and Lipid Composition of Cauliflower Plasma Membranes in Relation to ATPase Activity and Chilling Sensitivity Open
A plasma-membrane fraction rich in ion-stimulated ATPase activity was isolated from cauliflower (Brassica oleracea L.) buds. The activity of the ATPase was dependent on Mg(2+) and stimulated 4-fold by K(+). The lipids of the membrane fract…
View article: Correlation between Changes in Mitochondrial Membranes of Artichoke Tubers and Their Hardening and Dormancy
Correlation between Changes in Mitochondrial Membranes of Artichoke Tubers and Their Hardening and Dormancy Open
Spin labeling studies using mitochondrial membranes of Jerusalem artichoke (Helianthus tuberosus L.) showed that the decrease during winter in the temperatures of the upper and lower lipid transitions correlated with the development of fre…
View article: Maintenance of Membrane Fluidity during Development of Freezing Tolerance of Winter Wheat Seedlings
Maintenance of Membrane Fluidity during Development of Freezing Tolerance of Winter Wheat Seedlings Open
Fluidity of membrane lipids of shoot and root tissue and of chloroplasts from young wheat seedlings of contrasting freezing tolerance was investigated by measuring the motion and order parameters after spin labeling. A striking similarity …
View article: Seasonal Changes in the Structure and Function of Mitochondrial Membranes of Artichoke Tubers
Seasonal Changes in the Structure and Function of Mitochondrial Membranes of Artichoke Tubers Open
Changes in the temperature response, fluidity, function and the acyl fatty acid composition, were determined for a mitochondria-rich membrane fraction from Jerusalem artichoke (Helianthus tuberosus L.) tubers during dormancy for a crop whi…
View article: Seasonal Changes in the Structure and Function of Mitochondrial Membranes of Artichoke Tubers
Seasonal Changes in the Structure and Function of Mitochondrial Membranes of Artichoke Tubers Open
The temperature limits of the order-disorder transition, and the Arrhenius activation energy of succinate oxidase activity for mitochondria of Jerusalem artichoke (Helianthus tuberosus L.) tubers were determined from the initiation to the …
View article: Wheat Mitochondria
Wheat Mitochondria Open
Mitochondrial oxidative activity and membrane lipid structure of two wheat (Triticum aestivum L.) cultivars were measured as a function of temperature. The Arrhenius activation energy for the oxidation of both succinate and alpha-ketogluta…
View article: Occurrence of a Temperature-induced Phase Transition in Mitochondria Isolated from Apple Fruit
Occurrence of a Temperature-induced Phase Transition in Mitochondria Isolated from Apple Fruit Open
Mitochondria were isolated from fruit of six cultivars of apples differing in susceptibility to the physiological disorder, low temperature breakdown. The state 3 rate of succinate-dependent oxygen uptake and the motion of a spin label wer…
View article: Hibernation: Alteration of Mitochondrial Membranes as a Requisite for Metabolism at Low Temperature
Hibernation: Alteration of Mitochondrial Membranes as a Requisite for Metabolism at Low Temperature Open
Lipids of mitochondrial membranes from hepatic cells of active ground squirrels apparently go through a phase change induced at approximately 23°C similar to the phase change in mitochondria of homeotherms. This change was indicated by the…
View article: Temperature-induced Phase Changes in Mitochondrial Membranes Detected by Spin Labeling
Temperature-induced Phase Changes in Mitochondrial Membranes Detected by Spin Labeling Open
Molecular motion data from a spin-labeled fatty acid presented on Arrhenius graphs allow melting points or phase transitions to be inferred in local domains such as membranes. With use of this technique, mitochondria from a chilling-sensit…
View article: Oxidative Activity of Mitochondria Isolated from Plant Tissues Sensitive and Resistant to Chilling Injury
Oxidative Activity of Mitochondria Isolated from Plant Tissues Sensitive and Resistant to Chilling Injury Open
Arrhenius plots of the respiration rates of mitochondria isolated from chilling sensitive plant tissues (tomato and cucumber fruit, and sweet potato roots) showed a linear decrease from 25 C to about 9 to 12 C (with Q(10) values of 1.3 to …
View article: The Influence of Mitochondrial Concentration and Storage on the Respiratory Control of Isolated Plant Mitochondria
The Influence of Mitochondrial Concentration and Storage on the Respiratory Control of Isolated Plant Mitochondria Open
The respiration of mitochondria isolated from various plant tissues was studied over a range of mitochondrial concentrations and at various times after isolation. Respiration at 25 C expressed as nanomoles of O(2) per minute per milligram …