John M. McDowell
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View article: <scp>RNAi</scp> Strategies Against Downy Mildews: Insights Into <scp>dsRNA</scp> Uptake and Silencing
<span>RNAi</span> Strategies Against Downy Mildews: Insights Into <span>dsRNA</span> Uptake and Silencing Open
Downy mildew (DM) diseases are caused by destructive obligate pathogens with limited control options, posing a significant threat to global agriculture. RNA interference (RNAi) has emerged as a promising, environmentally sustainable strate…
View article: Advancing RNAi-Based Strategies Against Downy Mildews: Insights Into dsRNA Uptake and Gene Silencing
Advancing RNAi-Based Strategies Against Downy Mildews: Insights Into dsRNA Uptake and Gene Silencing Open
Downy mildew (DMs) dieases are caused by destructive obligate pathogens with limited control options, posing a significant threat to global agriculture. RNA interference (RNAi) has emerged as a promising, environmentally sustainable strate…
View article: Preserving the Biologically Coherent Generic Concept of <i>Phytophthora</i>, “Plant Destroyer”
Preserving the Biologically Coherent Generic Concept of <i>Phytophthora</i>, “Plant Destroyer” Open
Phytophthora is a long-established, well-known, and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from Phytophthora at least twice. Be…
View article: Synchronization of Circadian Clock Gene Expression in <i>Arabidopsis</i> and <i>Hyaloperonospora arabidopsidis</i> and its Impact on Host-Pathogen Interactions
Synchronization of Circadian Clock Gene Expression in <i>Arabidopsis</i> and <i>Hyaloperonospora arabidopsidis</i> and its Impact on Host-Pathogen Interactions Open
Organisms across all kingdoms have an internal circadian clock running in 24h cycles. This clock affects a variety of processes, including innate immunity in plants. However, the role of pathogen circadian clocks had not been extensively e…
View article: Small RNA-based plant protection against diseases
Small RNA-based plant protection against diseases Open
Plant diseases cause significant decreases in yield and quality of crops and consequently pose a very substantial threat to food security. In the continuous search for environmentally friendly crop protection, exploitation of RNA interfera…
A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification Open
SUMMARY Translating ribosome affinity purification (TRAP) utilizes transgenic plants expressing a ribosomal protein fused to a tag for affinity co‐purification of ribosomes and the mRNAs that they are translating. This population of active…
Peer Review #2 of "Functional analysis of a Phytophthora host-translocated effector using the yeast model system (v0.1)" Open
Background.Phytophthora plant pathogens secrete effector proteins that are translocated into host plant cells during infection and collectively contribute to pathogenicity.A subset of these hosttranslocated effectors can be identified by t…
A split GFP system to enhance spatial and temporal sensitivity of translating ribosome affinity purification (TRAP) Open
Summary Translating ribosome affinity purification (TRAP) utilizes transgenic plants expressing a ribosomal protein fused to a tag for affinity purification of ribosomes and the mRNAs that they are translating. These actively translated mR…
Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response Open
In addition to their role in the biosynthesis of important molecules such as proteins and specialized metabolites, amino acids are known to function as signaling molecules through various pathways to report nitrogen status and trigger appr…
Structure and Evolution of the Actin Gene Family in Arabidopsis Thaliana Open
Higher plants contain families of actin-encoding genes that are divergent and differentially expressed. Progress in understanding the functions and evolution of plant actins has been hindered by the large size of the actin gene families. I…
View article: The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression
The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression Open
Plant NLR-type receptors serve as sensitive triggers of host immunity. Their expression has to be well-balanced, due to their interference with various cellular processes and dose-dependency of their defense-inducing activity. A genetic "a…
Effect of light and dark on the growth and development of downy mildew pathogen <i>Hyaloperonospora arabidopsidis</i> Open
Disease development in plants requires a susceptible host, a virulent pathogen, and a favourable environment. Oomycete pathogens cause many important diseases and have evolved sophisticated molecular mechanisms to manipulate their hosts. D…
Increasing the Accuracy of Initial Feasibility Studies - Utilising Numerical Models to Estimate the LCoE of Floating Tidal Energy Platforms Open
This paper utilizes numerical modelling techniques to estimate the MetOcean conditions in regions with potential for tidal energy development.Understanding and incorporating these MetOcean site characteristics into the initial stages of a …
Effect of light and darkness on the growth and development of downy mildew pathogen <i>Hyaloperonospora arabidopsidis</i> Open
Summary Disease development in plants requires a susceptible host, a virulent pathogen, and a favourable environment. Oomycete pathogens cause many important diseases and have evolved sophisticated molecular mechanisms to manipulate their …
Draft Assembly of<i>Phytophthora capsici</i>from Long-Read Sequencing Uncovers Complexity Open
Resolving complex plant pathogen genomes is important for identifying the genomic shifts associated with rapid adaptation to selective agents such as hosts and fungicides, yet assembling these genomes remains challenging and expensive. Phy…
An oomycete RXLR effector triggers antagonistic plant hormone crosstalk to suppress host immunity Open
Understanding the mechanisms through which pathogens alter plant cell networks is essential for understanding plant-pathogen interactions and will inform efforts to reduce crop diseases. Oomycetes secrete diverse effector proteins into pla…
Oomycetes Used in Arabidopsis Research. Open
Arabidopsis plants in their natural environment are susceptible to infection by oomycete pathogens, in particular to downy mildew and white rust diseases. These naturally occurring infectious agents have imposed evolutionary pressures on A…
Focus on Activation, Regulation, and Evolution of MTI and ETI Open
Plants perceive a variety of molecules produced by microbes, insects, and nematodes. These pathogen-derived components include so-called microbe-associated molecular patterns, or MAMPs, as well as effector proteins that are secreted to the…
View article: The Arabidopsis <scp>RRM</scp> domain protein <scp>EDM</scp>3 mediates race‐specific disease resistance by controlling H3K9me2‐dependent alternative polyadenylation of <i><scp>RPP</scp>7</i> immune receptor transcripts
The Arabidopsis <span>RRM</span> domain protein <span>EDM</span>3 mediates race‐specific disease resistance by controlling H3K9me2‐dependent alternative polyadenylation of <i><span>RPP</span>7</i> immune receptor transcripts Open
Summary The NLR ‐receptor RPP 7 mediates race‐specific immunity in Arabidopsis. Previous screens for enhanced downy mildew ( edm ) mutants identified the co‐chaperone SGT 1b ( EDM 1) and the PHD ‐finger protein EDM 2 as critical regulators…
Application of alignment-free bioinformatics methods to identify an oomycete protein with structural and functional similarity to the bacterial AvrE effector protein Open
Diverse plant pathogens export effector proteins to reprogram host cells. One of the most challenging goals in the molecular plant-microbe field is to functionally characterize the complex repertoires of effectors secreted by these pathoge…
Focus on Effector-Triggered Susceptibility Open
Effector biology exhibits diversity at every level. Effector proteins play key roles in the molecular interplay between plants and plant-associated organisms, and effector biology remains one of the most active areas in the research field …
Conserved RxLR Effectors From Oomycetes <i>Hyaloperonospora arabidopsidis</i> and <i>Phytophthora sojae</i> Suppress PAMP- and Effector-Triggered Immunity in Diverse Plants Open
Effector proteins are exported to the interior of host cells by diverse plant pathogens. Many oomycete pathogens maintain large families of candidate effector genes, encoding proteins with a secretory leader followed by an RxLR motif. Alth…
Effector Biology in Focus: A Primer for Computational Prediction and Functional Characterization Open
Plant–pathogen interactions are controlled by a multilayered immune system, which is activated by pathogen recognition in the host. Pathogens secrete effector molecules to interfere with the immune recognition or signaling network and repr…
Zoospore exudates from Phytophthora nicotianae affect immune responses in Arabidopsis Open
Zoospore exudates play important roles in promoting zoospore communication, homing and germination during plant infection by Phytophthora. However, it is not clear whether exudates affect plant immunity. Zoospore-free fluid (ZFF) and zoosp…
View article: Foundational and Translational Research Opportunities to Improve Plant Health
Foundational and Translational Research Opportunities to Improve Plant Health Open
The white paper reports the deliberations of a workshop focused on biotic challenges to plant health held in Washington, D.C. in September 2016. Ensuring health of food plants is critical to maintaining the quality and productivity of crop…