John G. McCarron
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View article: VasoTracker 2: Open‐source software and hardware for tracking blood vessel diameter and assessing vascular function
VasoTracker 2: Open‐source software and hardware for tracking blood vessel diameter and assessing vascular function Open
VasoTracker 2 is a collection of open‐source tools for blood vessel diameter measurement and vascular physiology research, featuring automated diameter tracking software and low‐cost myography hardware components. This blood vessel analysi…
View article: Inside‐Out <scp>IP</scp> <sub>3</sub> ‐Mediated G Protein‐Coupled Receptor Activation Drives Intercellular Ca <sup>2+</sup> Signaling in the Vascular Endothelium
Inside‐Out <span>IP</span> <sub>3</sub> ‐Mediated G Protein‐Coupled Receptor Activation Drives Intercellular Ca <sup>2+</sup> Signaling in the Vascular Endothelium Open
The endothelium's control of nearly all vascular function relies on rapid intercellular communication to coordinate cellular activity across scale. A key form of intercellular communication arises from the regenerative propagation of IP 3 …
View article: Mitochondria regulate inositol triphosphate‐mediated Ca<sup>2+</sup> release triggered by voltage‐dependent Ca<sup>2+</sup> entry in resistance arteries
Mitochondria regulate inositol triphosphate‐mediated Ca<sup>2+</sup> release triggered by voltage‐dependent Ca<sup>2+</sup> entry in resistance arteries Open
An increase in cytoplasmic Ca 2+ concentration activates multiple cellular activities, including cell division, metabolism, growth, contraction and death. In smooth muscle Ca 2+ entry via voltage‐dependent Ca 2+ channels leads to a relativ…
View article: VasoTracker 2: An Open-source Platform for Quantitative Analysis of Vascular Reactivity and Function
VasoTracker 2: An Open-source Platform for Quantitative Analysis of Vascular Reactivity and Function Open
VasoTracker 2 is an open-source platform for studying blood vessel dynamics, featuring versatile diameter-tracking software and complementary low-cost hardware components. This system surpasses existing tools through accessible, high-resol…
View article: <i>Klebsiella pneumoniae</i> disrupts vasodilation by targeting eNOS post translational modifications via the type VI secretion system and the capsule polysaccharide
<i>Klebsiella pneumoniae</i> disrupts vasodilation by targeting eNOS post translational modifications via the type VI secretion system and the capsule polysaccharide Open
SUMMARY Vasodilation is a crucial protective response to inflammation and infection. Endothelial cells control vasodilation through the bioavailability of eNOS-produced nitric oxide (NO), and the generation of endothelium-dependent hyperpo…
View article: Increased TRPV4 Channel Expression Enhances and Impairs Blood Vessel Function in Hypertension
Increased TRPV4 Channel Expression Enhances and Impairs Blood Vessel Function in Hypertension Open
BACKGROUND: Endothelial cell TRPV4 (transient receptor potential vanilloid 4) channels provide a control point that is pivotal in regulating blood vessel diameter by mediating the Ca 2+ -dependent release of endothelial-derived vasoactive …
View article: Signalling switches maintain intercellular communication in the vascular endothelium
Signalling switches maintain intercellular communication in the vascular endothelium Open
Background and Purpose The single layer of cells lining all blood vessels, the endothelium, is a sophisticated signal co‐ordination centre that controls a wide range of vascular functions including the regulation of blood pressure and bloo…
View article: Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension
Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension Open
Background Endothelial cell TRPV4 channels provide a control point that is pivotal in regulating blood vessel diameter by mediating the Ca 2+ -dependent release of endothelial-derived vasoactive factors. In hypertension, TRPV4-mediated con…
View article: Endothelial PAR2 activation evokes resistance artery relaxation
Endothelial PAR2 activation evokes resistance artery relaxation Open
Protease‐activated receptor‐1 & ‐2 (PAR1 and PAR2) are expressed widely in cardiovascular tissues including endothelial and smooth muscle cells. PAR1 and PAR2 may regulate blood pressure via changes in vascular contraction or relaxation me…
View article: Mitochondrial ATP Production is Required for Endothelial Cell Control of Vascular Tone
Mitochondrial ATP Production is Required for Endothelial Cell Control of Vascular Tone Open
Arteries and veins are lined by nonproliferating endothelial cells that play a critical role in regulating blood flow. Endothelial cells also regulate tissue perfusion, metabolite exchange, and thrombosis. It is thought that endothelial ce…
View article: William Bayliss and the enduring fascination of myogenic tone
William Bayliss and the enduring fascination of myogenic tone Open
'A new scientific truth does not triumph by convincing its opponents and making them see the light, but rather because its opponents eventually die' Given the pace of scientific progress over the seven decades since this much-quoted observ…
View article: Small-world connectivity dictates collective endothelial cell signaling
Small-world connectivity dictates collective endothelial cell signaling Open
Significance The endothelium is the single layer of cells lining all blood vessels and acts as a central control hub to regulate multiple cardiovascular functions in response to hundreds of physiological stimuli. The detection of various p…
View article: Mitochondria regulate TRPV4‐mediated release of ATP
Mitochondria regulate TRPV4‐mediated release of ATP Open
Background and Purpose Ca 2+ influx via TRPV4 channels triggers Ca 2+ release from the IP 3 ‐sensitive internal store to generate repetitive oscillations. Although mitochondria are acknowledged regulators of IP 3 ‐mediated Ca 2+ release, h…
View article: Mitochondrial ATP production is required for endothelial cell control of vascular tone
Mitochondrial ATP production is required for endothelial cell control of vascular tone Open
Arteries and veins are lined by non-proliferating endothelial cells that play a critical role in regulating blood flow. Endothelial cells also regulate tissue perfusion, metabolite exchange, and thrombosis. It is thought that endothelial c…
View article: BS22 TRPV4 is a phospholipase C coupled receptor when mitochondria are depolarised in intact endothelial cells
BS22 TRPV4 is a phospholipase C coupled receptor when mitochondria are depolarised in intact endothelial cells Open
Mitochondria are significant regulators of intracellular Ca2+ signalling in the vascular endothelium. Depolarization of the mitochondrial membrane potential (Δψm) inhibits IP3-mediated Ca2+ release and may regulate Ca2+ influx across the …
View article: Mitochondria regulate TRPV4 mediated release of ATP
Mitochondria regulate TRPV4 mediated release of ATP Open
Background and Purpose Ca influx via TRPV4 triggers Ca release from the IP-sensitive internal store to generate repetitive oscillations. While mitochondria are acknowledged regulators of IP-mediated Ca release, how TRPV4-mediated Ca signal…
View article: Photoactivated release of membrane impermeant sulfonates inside cells
Photoactivated release of membrane impermeant sulfonates inside cells Open
Photocaged sulfonate delivers membrane impermeant compounds to cells.
View article: Carbenoxolone and 18β‐glycyrrhetinic acid inhibit inositol 1,4,5‐trisphosphate‐mediated endothelial cell calcium signalling and depolarise mitochondria
Carbenoxolone and 18β‐glycyrrhetinic acid inhibit inositol 1,4,5‐trisphosphate‐mediated endothelial cell calcium signalling and depolarise mitochondria Open
Background and Purpose Coordinated endothelial control of cardiovascular function is proposed to occur by endothelial cell communication via gap junctions and connexins. To study intercellular communication, the pharmacological agents carb…
View article: Carbenoxolone and 18β-Glycyrrhetinic Acid Inhibit IP3-Mediated Endothelial Cell Calcium Signalling and Depolarise Mitochondria
Carbenoxolone and 18β-Glycyrrhetinic Acid Inhibit IP3-Mediated Endothelial Cell Calcium Signalling and Depolarise Mitochondria Open
Background and Purpose Coordinated endothelial control of cardiovascular function is proposed to occur by endothelial cell communication via gap junctions and connexins. To study intercellular communication, the pharmacological agents carb…
View article: Disrupted Endothelial Cell Heterogeneity and Network Organization Impairs Vascular Function in Prediabetic Obesity
Disrupted Endothelial Cell Heterogeneity and Network Organization Impairs Vascular Function in Prediabetic Obesity Open
Rationale Obesity is a major risk factor for diabetes and cardiovascular diseases such as hypertension, heart failure, and stroke. Impaired endothelial function occurs in the earliest stages of obesity and underlies vascular alterations gi…
View article: FK506 regulates Ca<sup>2+</sup> release evoked by inositol 1,4,5‐trisphosphate independently of FK‐binding protein in endothelial cells
FK506 regulates Ca<sup>2+</sup> release evoked by inositol 1,4,5‐trisphosphate independently of FK‐binding protein in endothelial cells Open
Background and Purpose FK506 and rapamycin are modulators of FK‐binding proteins (FKBP) that are used to suppress immune function after organ and hematopoietic stem cell transplantations. The drugs share the unwanted side‐effect of evoking…
View article: Multi-plane remote refocusing epifluorescence microscopy to image dynamic Ca<i/><sup>2+</sup>events
Multi-plane remote refocusing epifluorescence microscopy to image dynamic Ca<i/><sup>2+</sup>events Open
Rapid imaging of multiple focal planes without sample movement may be achieved through remote refocusing, where imaging is carried out in a plane conjugate to the sample plane. The technique is ideally suited to studying the endothelial an…
View article: Increased Vascular Contractility in Hypertension Results From Impaired Endothelial Calcium Signaling
Increased Vascular Contractility in Hypertension Results From Impaired Endothelial Calcium Signaling Open
Endothelial cells line all blood vessels and are critical regulators of vascular tone. In hypertension, disruption of endothelial function alters the release of endothelial-derived vasoactive factors and results in increased vascular tone.…
View article: Endothelial TRPV4 channels modulate vascular tone by Ca <sup>2+</sup> ‐induced Ca <sup>2+</sup> release at inositol 1,4,5‐trisphosphate receptors
Endothelial TRPV4 channels modulate vascular tone by Ca <sup>2+</sup> ‐induced Ca <sup>2+</sup> release at inositol 1,4,5‐trisphosphate receptors Open
Background and Purpose The TRPV4 ion channels are Ca 2+ permeable, non‐selective cation channels that mediate large, but highly localized, Ca 2+ signals in the endothelium. The mechanisms that permit highly localized Ca 2+ changes to evoke…
View article: Heterogeneity and emergent behaviour in the vascular endothelium
Heterogeneity and emergent behaviour in the vascular endothelium Open
The endothelium is the single layer of cells lining all blood vessels, and it is a remarkable cardiovascular control centre. Each endothelial cell has only a small number (on average six) of interconnected neighbours. Yet this arrangement …
View article: VasoTracker, a Low-Cost and Open Source Pressure Myograph System for Vascular Physiology
VasoTracker, a Low-Cost and Open Source Pressure Myograph System for Vascular Physiology Open
Pressure myography, one of the most commonly used techniques in vascular research, measures the diameter of isolated, pressurized arteries to assess the functional activity of smooth muscle and endothelial cells. Despite the widespread ado…