John A. McLean
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View article: Air Pollution Exposure and Birth Weight in the ECHO Cohort
Air Pollution Exposure and Birth Weight in the ECHO Cohort Open
Importance Prior studies report negative associations between prenatal exposure to fine particulate matter (ie, aerodynamic diameter 2.5 µg; PM 2.5 ) and birth weight, but have typically averaged exposure across pregnancy, which may not re…
Disruption of acyl-acyl carrier protein (acyl-ACP) synthetase in cyanobacteria impairs lipid remodeling as revealed by acyl-ACP measurements Open
Free fatty acid (FFA) production in bacteria is a key target for metabolic engineering. The knockout of the acyl-ACP synthetase (AAS) prevents reincorporation of FFA into the fatty acid biosynthetic cycle and is widely used to enhance thei…
View article: COVID-19 Pandemic Exposure and Toddler Behavioral Health in the ECHO Program
COVID-19 Pandemic Exposure and Toddler Behavioral Health in the ECHO Program Open
Importance Studies suggest developmental concerns for infants born during the COVID-19 pandemic, but evidence on its impact on toddler behavioral and emotional well-being remains limited. Objective To assess whether birth timing relative t…
Active Humidity Control Chamber for Desorption Electrospray Ionization-Mass Spectrometry Imaging Applications Open
Ambient ionization techniques enable mass spectrometry (MS) to expand into broader experimental contexts, although it is increasingly clear that results are influenced by the environmental conditions at the site of sampling. Desorption ele…
View article: Dehydration promotes intracellular lipid synthesis and accumulation
Dehydration promotes intracellular lipid synthesis and accumulation Open
Lipids can be considered a water reservoir used to offset dehydration stress as their oxidation by the mitochondria generates water. However, whether dehydration directly regulates lipid synthesis is unknown, which is the focus of this stu…
View article: Rapid Annotation Strategy for <i>in Vivo</i> Phase II Metabolites of Anabolic–Androgenic Steroids Using Liquid Chromatography–Ion Mobility–Mass Spectrometry
Rapid Annotation Strategy for <i>in Vivo</i> Phase II Metabolites of Anabolic–Androgenic Steroids Using Liquid Chromatography–Ion Mobility–Mass Spectrometry Open
Doping control laboratories are responsible for the precise measurement of anabolic-androgenic steroids (AASs) and determination of athlete usage. Intact phase II AASs are difficult to analyze due to their low abundance in complex biologic…
Photoaffinity Ligand of Cystic Fibrosis Corrector VX-445 Identifies SCCPDH as an Off-Target Open
Cystic fibrosis (CF) pharmacological correctors improve the cystic fibrosis transmembrane conductance regulator (CFTR) protein trafficking and function. Several side effects of these correctors and adverse drug interactions have been repor…
View article: Public Water Arsenic and Birth Outcomes in the Environmental Influences on Child Health Outcomes Cohort
Public Water Arsenic and Birth Outcomes in the Environmental Influences on Child Health Outcomes Cohort Open
Importance Inorganic arsenic is associated with adverse birth outcomes, but evidence is limited for public water concentrations (modifiable by federal regulatory action) in US populations. Objective To evaluate the association between pren…
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates Open
The enhancement of antitumour immunity via agonists of the stimulator of interferon genes (STING) pathway is limited by pharmacological barriers. Here we show that the covalent conjugation of a STING agonist to anti-albumin nanobodies via …
Tissue integrity impacts of application of 160–200 kJ of 915 MHz microwave energy, using the DTS: Diathermic Syncope® system, to the forehead of cattle, and alignment with the requirements of religious slaughter markets Open
Although society desires good animal welfare at slaughter, there is a tension between some religious requirements and modern commercial stunning methods. The DTS (Diathermic Syncope®) system has been designed with a vision of relieving tha…
View article: An Anaerobic Pathogen Rewires Host Metabolism to Fuel Oxidative Growth in the Inflamed Gut
An Anaerobic Pathogen Rewires Host Metabolism to Fuel Oxidative Growth in the Inflamed Gut Open
To colonize their host and cause disease, enteric pathogens must deploy their virulence factors to establish distinct nutrient niches. How obligate anaerobic pathogens construct nutrient niches in the densely populated large intestine rema…
View article: Maternal Cardiometabolic Risk Factors in Pregnancy and Offspring Blood Pressure at Age 2 to 18 Years
Maternal Cardiometabolic Risk Factors in Pregnancy and Offspring Blood Pressure at Age 2 to 18 Years Open
Importance Higher blood pressure in early life may signal cardiovascular disease over the life course, but determinants of blood pressure in early life are poorly understood. Objective To examine the association of maternal cardiometabolic…
View article: Short-term alterations in dietary amino acids override host genetic susceptibility and reveal mechanisms of <i>Salmonella</i> Typhimurium small intestine colonization
Short-term alterations in dietary amino acids override host genetic susceptibility and reveal mechanisms of <i>Salmonella</i> Typhimurium small intestine colonization Open
Summary In addition to individual genetics, environmental factors (e.g., dietary changes) may influence host susceptibility to gastrointestinal infection through unknown mechanisms. Herein, we developed a model in which CBA/J mice, a genet…
Photoaffinity ligand of Cystic Fibrosis corrector VX-445 identifies SCCPDH as an off-target Open
Cystic fibrosis (CF) pharmacological correctors improve Cystic Fibrosis transmembrane conductance regulator (CFTR) protein trafficking and function. Several side-effects from these correctors and adverse drug interactions have been reporte…
View article: Racial and ethnic differences in prenatal exposure to environmental phenols and parabens in the ECHO Cohort
Racial and ethnic differences in prenatal exposure to environmental phenols and parabens in the ECHO Cohort Open
Background Research suggests racial/ethnic disparities in prenatal exposure to endocrine disrupting environmental phenols (EPs) in limited populations. However, no studies have investigated racial/ethnic disparities in prenatal EP exposure…
View article: A Standardized Nontargeted Metabolomics Analytical Method for Qualitatively Comparing Apples to Apples
A Standardized Nontargeted Metabolomics Analytical Method for Qualitatively Comparing Apples to Apples Open
Food composition has been traditionally defined by 35-160 chemical components with established nutritional significance for human health. Modern omics technologies have revealed that the chemical complexity of food is far greater, offering…
A Fast-Pass, Desorption Electrospray Ionization Mass Spectrometry Strategy for Untargeted Metabolic Phenotyping Open
Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) provides direct analytical readouts of small molecules that can be used to characterize the metabolic phenotypes of genetically engineered bacteria. In an effort to ac…
Structural characterization of cyclodextrin and maltodextrin alkali-metal coordination <i>via</i> ion mobility-mass spectrometry and computational modeling Open
Structurally-selective ion mobility-mass spectrometry and complementary computational modeling were implemented to probe the gas-phase conformations of cyclodextrin and maltodextrin alkali-metal host–guest inclusion complexes.
View article: Associations between a novel measure of sleep health and cognitive functioning in middle childhood: a crosssectional Environmental Influences on Child Health Outcomes cohort study
Associations between a novel measure of sleep health and cognitive functioning in middle childhood: a crosssectional Environmental Influences on Child Health Outcomes cohort study Open
Study Objectives Research linking children’s sleep to cognitive outcomes is inconsistent and has largely focused on one aspect of sleep, such as duration, rather than measuring multiple dimensions of sleep health. We hypothesized that chil…
Spatiochemical Investigation of Potential Biocontrol Agents Against <i>Phytophthora capsici</i> Infection in Tomato Open
Biological control agents can offer an eco-friendly and more sustainable alternative to conventional chemical pesticides, providing protection against destructive pathogens, such as Phytophthora capsici, while reducing potential environmen…
View article: Prenatal Diet and Infant Growth From Birth to Age 24 Months
Prenatal Diet and Infant Growth From Birth to Age 24 Months Open
Importance Being born either small for gestational age (SGA) or large for gestational age (LGA) and experiencing rapid or slow growth after birth are associated with later-life obesity. Understanding the associations of dietary quality dur…
View article: Neighborhood Food Access in Early Life and Trajectories of Child Body Mass Index and Obesity
Neighborhood Food Access in Early Life and Trajectories of Child Body Mass Index and Obesity Open
Importance Limited access to healthy foods, resulting from residence in neighborhoods with low food access, is a public health concern. The contribution of this exposure in early life to child obesity remains uncertain. Objective To examin…
An Untargeted Lipidomics Workflow Incorporating High-Resolution Demultiplexing (HRdm) Drift Tube Ion Mobility-Mass Spectrometry Open
Global discovery lipidomics can provide comprehensive chemical information toward understanding the intricacies of metabolic lipid disorders such as dyslipidemia; however, the isomeric complexity of lipid species remains an analytical chal…
ENDOTHELIAL-DEPENDENT VASCULAR REACTIVITY AFTER CARDIOPULMONARY BYPASS IS ASSOCIATED WITH UNIQUE METABOLOMIC SIGNATURES Open
Cardiopulmonary bypass (CPB), an extracorporeal method necessary for the surgical correction of complex congenital heart defects, incites significant inflammation that affects vascular function. These changes are associated with alteration…
Direct Enantiomer Differentiation of Drugs and Drug-Like Compounds via Noncovalent Copper–Amino Acid Complexation and Ion Mobility-Mass Spectrometry Open
Drug enantiomers can possess vastly different pharmacological properties, yet they are identical in their chemical composition and structural connectivity. Thus, resolving enantiomers poses a great challenge in the field of separation scie…
Ion Mobility-Mass Spectrometry Strategies to Elucidate the Anhydrous Structure of Noncovalent Guest/Host Complexes Open
Structural mass spectrometry (MS) techniques are fast and sensitive analytical methods to identify noncovalent guest/host complexation phenomena for desirable solution-phase properties. Current MS-based studies on guest/host complexes of d…
View article: Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction
Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction Open
Glutamine metabolism is altered in both infarct macrophages and the remote left ventricle (LV) following myocardial infarction (MI). Supplemental glutamine improves LV function following MI while inhibiting glutamine metabolism with BPTES …
Taurine modulates host cell responses to <i>Helicobacter pylori</i> VacA toxin Open
Colonization of the human stomach with Helicobacter pylori strains producing active forms of the secreted toxin VacA is associated with an increased risk of peptic ulcer disease and gastric cancer, compared with colonization with strains p…
High-resolution ion mobility based on traveling wave structures for lossless ion manipulation resolves hidden lipid features Open
High-resolution ion mobility (resolving power > 200) coupled with mass spectrometry (MS) is a powerful analytical tool for resolving isobars and isomers in complex samples. High-resolution ion mobility is capable of discerning additional s…