John Termini
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View article: Determining the Mechanism of DJ-1 Using Mix-and-Inject Synchrotron Serial Crystallography
Determining the Mechanism of DJ-1 Using Mix-and-Inject Synchrotron Serial Crystallography Open
Determining the molecular basis of enzyme catalytic activity is a central challenge in biochemistry. Time-resolved serial X-ray crystallography using X-ray free electron (XFELs) and synchrotron sources allows enzyme reactions to be observe…
View article: Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron
Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron Open
DJ-1 (PARK7) is an intensively studied protein whose cytoprotective activities are dysregulated in multiple diseases. DJ-1 has been reported as having two distinct enzymatic activities in defense against reactive carbonyl species that are …
View article: <b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b>
<b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b> Open
AbstractOver 30% of patients with type 1 diabetes develop diabetic kidney disease (DKD), significantly increasing mortality risk. The Diabetes Control and Complications Trial (DCCT) and follow-up study Epidemiology of Diabetes Intervention…
View article: Methylglyoxal Adducts Are Prognostic Biomarkers for Diabetic Kidney Disease in Patients With Type 1 Diabetes
Methylglyoxal Adducts Are Prognostic Biomarkers for Diabetic Kidney Disease in Patients With Type 1 Diabetes Open
More than 30% of patients with type 1 diabetes develop diabetic kidney disease (DKD), which significantly increases mortality risk. The Diabetes Control and Complications Trial (DCCT) and follow-up study, Epidemiology of Diabetes Intervent…
View article: <b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b>
<b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b> Open
AbstractOver 30% of patients with type 1 diabetes develop diabetic kidney disease (DKD), significantly increasing mortality risk. The Diabetes Control and Complications Trial (DCCT) and follow-up study Epidemiology of Diabetes Intervention…
View article: <b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b>
<b>Methylglyoxal adducts are prognostic biomarkers for diabetic kidney disease in patients with type 1 diabetes</b> Open
AbstractOver 30% of patients with type 1 diabetes develop diabetic kidney disease (DKD), significantly increasing mortality risk. The Diabetes Control and Complications Trial (DCCT) and follow-up study Epidemiology of Diabetes Intervention…
View article: Methylglyoxal products in pre-symptomatic type 1 diabetes
Methylglyoxal products in pre-symptomatic type 1 diabetes Open
Introduction Progression to type 1 diabetes has emerged as a complex process with metabolic alterations proposed to be a significant driver of disease. Monitoring products of altered metabolism is a promising tool for determining the risk …
View article: <scp>DJ</scp> ‐1 is not a deglycase and makes a modest contribution to cellular defense against methylglyoxal damage in neurons
<span>DJ</span> ‐1 is not a deglycase and makes a modest contribution to cellular defense against methylglyoxal damage in neurons Open
Human DJ‐1 is a cytoprotective protein whose absence causes Parkinson's disease and is also associated with other diseases. DJ‐1 has an established role as a redox‐regulated protein that defends against oxidative stress and mitochondrial d…
View article: DJ-1 glyoxalase activity makes a modest contribution to cellular defense against methylglyoxal damage in neurons
DJ-1 glyoxalase activity makes a modest contribution to cellular defense against methylglyoxal damage in neurons Open
Human DJ-1 is a cytoprotective protein whose absence causes Parkinson’s disease and is also associated with other diseases. DJ-1 has an established role as a redox-regulated protein that defends against oxidative stress and mitochondrial d…
View article: Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation
Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation Open
The integration of cellular status with metabolism is critically important and the coupling of energy production and cellular function is highly evolutionarily conserved. This has been demonstrated in stem cell biology, organismal, cellula…
View article: Elevated glucose increases genomic instability by inhibiting nucleotide excision repair
Elevated glucose increases genomic instability by inhibiting nucleotide excision repair Open
We investigated potential mechanisms by which elevated glucose may promote genomic instability. Gene expression studies, protein measurements, mass spectroscopic analyses, and functional assays revealed that elevated glucose inhibited the …
View article: Protein-coated corrole nanoparticles for the treatment of prostate cancer cells
Protein-coated corrole nanoparticles for the treatment of prostate cancer cells Open
Development of novel therapeutic strategies to eradicate malignant tumors is of paramount importance in cancer research. In a recent study, we have introduced a facile protocol for the preparation of corrole-protein nanoparticles (NPs). Th…
View article: Metal-Assisted Protein Quantitation (MAPq): Multiplex Analysis of Protein Expression Using Lanthanide-Modified Antibodies with Detection by Inductively Coupled Plasma Mass Spectrometry
Metal-Assisted Protein Quantitation (MAPq): Multiplex Analysis of Protein Expression Using Lanthanide-Modified Antibodies with Detection by Inductively Coupled Plasma Mass Spectrometry Open
Understanding the complex relationships between genomics, transcriptomics, and proteomics requires the development of more sensitive and rapid methods of multiplexed protein analysis. This is necessary to understand the relationship betwee…
View article: Structures and Spectroscopic Properties of Metallocorrole Nanoparticles
Structures and Spectroscopic Properties of Metallocorrole Nanoparticles Open
In aqueous media, hydrophobic metallocorroles form nanoparticles that are potential theranostic anticancer agents. We have analyzed the electronic and Raman spectra of Al(III), Ga(III), and Au(III) corrole nanoparticles (and made compariso…
View article: MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein
MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein Open
Protein glycosylation is a diverse form of post-translational modification. Two to three consecutive O-linked N-acetylgalactosamines (Tn-antigens) are recognized by antibodies such as MLS128. MLS128 mAb inhibited cell growth and bound to a…
View article: Inhibition of GLO1 in Glioblastoma Multiforme Increases DNA-AGEs, Stimulates RAGE Expression, and Inhibits Brain Tumor Growth in Orthotopic Mouse Models
Inhibition of GLO1 in Glioblastoma Multiforme Increases DNA-AGEs, Stimulates RAGE Expression, and Inhibits Brain Tumor Growth in Orthotopic Mouse Models Open
Cancers that exhibit the Warburg effect may elevate expression of glyoxylase 1 (GLO1) to detoxify the toxic glycolytic byproduct methylglyoxal (MG) and inhibit the formation of pro-apoptotic advanced glycation endproducts (AGEs). Inhibitio…
View article: Product Studies and Mechanistic Analysis of the Reaction of Methylglyoxal with Deoxyguanosine
Product Studies and Mechanistic Analysis of the Reaction of Methylglyoxal with Deoxyguanosine Open
Methylglyoxal (MG) is a highly reactive electrophile produced endogenously as a byproduct of glucose metabolism and protein catabolism and exogenously as a food contaminant. MG reacts spontaneously with proteins, lipids, and nucleic acids …
View article: Fighting Cancer with Corroles
Fighting Cancer with Corroles Open
Corroles are exceptionally promising platforms for the development of agents for simultaneous cancer-targeting imaging and therapy. Depending on the element chelated by the corrole, these theranostic agents may be tuned primarily for diagn…
View article: Cellular uptake and anticancer activity of carboxylated gallium corroles
Cellular uptake and anticancer activity of carboxylated gallium corroles Open
Significance Corroles are macrocyclic molecules related to porphyrins that exhibit intense light absorptions in the visible region. They also are very bright emitters, with luminescence quantum yields over 50% in some cases. Importantly, w…
View article: Lanthanides: Applications in Cancer Diagnosis and Therapy
Lanthanides: Applications in Cancer Diagnosis and Therapy Open
Lanthanide complexes are of increasing importance in cancer diagnosis and therapy, owing to the versatile chemical and magnetic properties of the lanthanide-ion 4f electronic configuration. Following the first implementation of gadolinium(…
View article: An <em>In Vitro</em> Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H<sub>3</sub> 5,10,15-tris(pentafluorophenyl)corroles
An <em>In Vitro</em> Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H<sub>3</sub> 5,10,15-tris(pentafluorophenyl)corroles Open
Chemotherapy often involves broad-spectrum cytotoxic agents with many side effects and limited targeting. Corroles are a class of tetrapyrrolic macrocycles that exhibit differential cytostatic and cytotoxic properties in specific cell line…