John Turek
YOU?
Author Swipe
View article: Exploring tumor dynamics and responses of prostate cancer to IL-27 based treatment combinations through biodynamic imaging and RNA sequencing analyses
Exploring tumor dynamics and responses of prostate cancer to IL-27 based treatment combinations through biodynamic imaging and RNA sequencing analyses Open
The tumor microenvironment (TME) is a highly dynamic network shaped by immune, vascular, and stromal interactions, further modulated by extracellular factors. The high tumor heterogeneity complicates treatment due to evolving resistance. B…
View article: Intracellular doppler spectroscopy of live tissue sentinels for a fast in-vitro bacterial infection assay
Intracellular doppler spectroscopy of live tissue sentinels for a fast in-vitro bacterial infection assay Open
Living organisms are frequently used as sentinels to monitor changes in the environment, and organoids can serve a similar function as in vitro sentinels detecting microbiological processes. One such process of great interest is pathogenic…
View article: Evaluating the feasibility and predictive accuracy of biodynamic imaging to platinum-based chemotherapy response in esophageal adenocarcinoma
Evaluating the feasibility and predictive accuracy of biodynamic imaging to platinum-based chemotherapy response in esophageal adenocarcinoma Open
Background Esophageal cancer management lacks reliable response predictors to chemotherapy. In this study we evaluated the feasibility and accuracy of Biodynamic Imaging (BDI), a technology that employs digital holography as a rapid predic…
View article: Comparative oncology chemosensitivity assay for personalized medicine using low-coherence digital holography of dynamic light scattering from cancer biopsies
Comparative oncology chemosensitivity assay for personalized medicine using low-coherence digital holography of dynamic light scattering from cancer biopsies Open
Nearly half of cancer patients who receive standard-of-care treatments fail to respond to their first-line chemotherapy, demonstrating the pressing need for improved methods to select personalized cancer therapies. Low-coherence digital ho…
View article: Chemosensitivity testing of revived fresh-frozen biopsies using digital speckle holography
Chemosensitivity testing of revived fresh-frozen biopsies using digital speckle holography Open
Enrolling patients in clinical trials to obtain fresh tumor biopsies to profile anticancer agents can be slow and expensive. However, if flash-frozen biopsies can be thawed to produce viable living tissue with relevant biodynamic profiles,…
View article: Common-path interferometer for digital holographic Doppler spectroscopy of living biological tissues
Common-path interferometer for digital holographic Doppler spectroscopy of living biological tissues Open
A common-path interferometer using a grating and an SF can provide enhanced interferometric stability in tissue-dynamics spectroscopy for drug screening assays.
View article: Doppler imaging detects bacterial infection of living tissue
Doppler imaging detects bacterial infection of living tissue Open
Living 3D in vitro tissue cultures, grown from immortalized cell lines, act as living sentinels as pathogenic bacteria invade the tissue. The infection is reported through changes in the intracellular dynamics of the sentinel cells caused …
View article: Biodynamic signatures from ex vivo bone marrow aspirates are associated with chemotherapy‐induced neutropenia in cancer‐bearing dogs
Biodynamic signatures from ex vivo bone marrow aspirates are associated with chemotherapy‐induced neutropenia in cancer‐bearing dogs Open
Background Neutropenia is the most common dose‐limiting side effect of cytotoxic chemotherapy in cancer‐bearing dogs. Biodynamic imaging (BDI) is a functional imaging technology that measures dynamic light scattering from living, three‐dim…
View article: Biodynamic digital holographic speckle microscopy for oocyte and embryo metabolic evaluation
Biodynamic digital holographic speckle microscopy for oocyte and embryo metabolic evaluation Open
Assisted reproductive technologies seek to improve the success rate of pregnancies. Morphology scoring is a common approach to evaluate oocyte and embryo viability prior to embryo transfer in utero , but the efficacy of the method is low. …
View article: Tissue Dynamics Spectroscopic Imaging: Functional Imaging of Heterogeneous Cancer Tissue
Tissue Dynamics Spectroscopic Imaging: Functional Imaging of Heterogeneous Cancer Tissue Open
Significance Tumor heterogeneity poses a challenge for the chemotherapeutic treatment of cancer. Tissue dynamics spectroscopy (TDS) captures dynamic contrast and can capture the response of living tissue to applied therapeutics, but the cu…
View article: Tissue dynamics spectroscopic imaging: functional imaging of heterogeneous cancer tissue
Tissue dynamics spectroscopic imaging: functional imaging of heterogeneous cancer tissue Open
We have demonstrated the utility of TDSI as a functional imaging method to measure tumor heterogeneity and its potential for use in drug-response profiling.
View article: Intracellular Optical Doppler Phenotypes of Chemosensitivity in Human Epithelial Ovarian Cancer
Intracellular Optical Doppler Phenotypes of Chemosensitivity in Human Epithelial Ovarian Cancer Open
Development of an assay to predict response to chemotherapy has remained an elusive goal in cancer research. We report a phenotypic chemosensitivity assay for epithelial ovarian cancer based on Doppler spectroscopy of infrared light scatte…
View article: Integration of Biodynamic Imaging and RNA-seq classifies chemotherapy response in canine diffuse large B-cell lymphoma
Integration of Biodynamic Imaging and RNA-seq classifies chemotherapy response in canine diffuse large B-cell lymphoma Open
Diffuse large B-cell lymphoma (DLBCL) is a common, aggressive cancer of notorious genotypic and phenotypic heterogeneity. A major challenge is predicting response to drug treatment, which has typically been done using genomic tools alone w…
View article: Doppler Fluctuation Spectroscopy of Intracellular Dynamics in Living Tissue
Doppler Fluctuation Spectroscopy of Intracellular Dynamics in Living Tissue Open
Intracellular dynamics in living tissue are dominated by active transport driven by bioenergetic processes far from thermal equilibrium. Intracellular constituents typically execute persistent walks. In the limit of long mean-free paths, t…
View article: Doppler fluctuation spectroscopy of intracellular dynamics in living tissue
Doppler fluctuation spectroscopy of intracellular dynamics in living tissue Open
Intracellular dynamics in living tissue are dominated by active transport driven by bioenergetic processes far from thermal equilibrium. Intracellular constituents typically execute persistent walks. In the limit of long mean free paths, t…
View article: Biodynamic digital holography of chemoresistance in a pre-clinical trial of canine B-cell lymphoma
Biodynamic digital holography of chemoresistance in a pre-clinical trial of canine B-cell lymphoma Open
Biodynamic digital holography was used to obtain phenotypic profiles of canine non-Hodgkin B-cell lymphoma biopsies treated with standard-of-care chemotherapy. Biodynamic signatures from the living 3D tissues were extracted using fluctuati…
View article: Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture
Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture Open
Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging …
View article: Intracellular Doppler Signatures of Platinum Sensitivity Captured by Biodynamic Profiling in Ovarian Xenografts
Intracellular Doppler Signatures of Platinum Sensitivity Captured by Biodynamic Profiling in Ovarian Xenografts Open
Three-dimensional (3D) tissue cultures are replacing conventional two-dimensional (2D) cultures for applications in cancer drug development. However, direct comparisons of in vitro 3D models relative to in vivo models derived from the same…