Andrew J. Weightman
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View article: Genome-resolved metagenomics uncovers antimicrobial resistance gene carriers in hospital and municipal wastewater environments
Genome-resolved metagenomics uncovers antimicrobial resistance gene carriers in hospital and municipal wastewater environments Open
Wastewater-based epidemiology (WBE) is a powerful approach to study antimicrobial resistance (AMR) dynamics at the population level. Using genome-resolved metagenomics, we recovered 3978 metagenome-assembled genomes (MAGs) from archived me…
View article: Assessing the risk of antimicrobial resistance and potential environmental harm through national-scale surveillance of antimicrobials in hospital and community wastewater
Assessing the risk of antimicrobial resistance and potential environmental harm through national-scale surveillance of antimicrobials in hospital and community wastewater Open
Antimicrobial resistance (AMR) is a major public health concern. Antimicrobial residues enter wastewater where their continued presence can lead to an increased risk of AMR while also causing environmental harm when untreated wastewater is…
View article: Metagenomic profiling of hospital wastewater: A comprehensive national scale analysis of antimicrobial resistance genes and opportunistic pathogens
Metagenomic profiling of hospital wastewater: A comprehensive national scale analysis of antimicrobial resistance genes and opportunistic pathogens Open
Overall, the study highlights the effectiveness of combining wastewater-based epidemiology with metagenomics to gain critical insights into the distinct resistome and microbiome profiles in hospital settings. Tailored strategies are essent…
View article: Toileting behaviours of the UK public: insights for reducing demographic bias in wastewater-based epidemiology sampling strategies
Toileting behaviours of the UK public: insights for reducing demographic bias in wastewater-based epidemiology sampling strategies Open
Wastewater-based epidemiology has gained increasing interest for its application as a public health surveillance tool, yet the impact of toileting habits over the representativeness of wastewater data remains underexplored. To address this…
View article: Complete genome sequence of <i>Pseudomonas</i> sp. PP3, a dehalogenase-producing bacterium, confirms the unusual mobile genetic element <i>DEH</i>
Complete genome sequence of <i>Pseudomonas</i> sp. PP3, a dehalogenase-producing bacterium, confirms the unusual mobile genetic element <i>DEH</i> Open
Pseudomonas sp. PP3, originally isolated from contaminated soil enriched in a chemostat culture on 2,2-dichloropropionic acid, has a 6.42 Mb genome, most closely related to P. reinekei . This well-characterized organism continues to provid…
View article: <i>Inocybe crenata</i> sp. nov. (subsec. <i>Geophyllinae</i> , sect. <i>Tardae</i> ) from Conifer Dominated Forests of Pakistan
<i>Inocybe crenata</i> sp. nov. (subsec. <i>Geophyllinae</i> , sect. <i>Tardae</i> ) from Conifer Dominated Forests of Pakistan Open
Inocybe crenata sp. nov. (subsec. Geophyllinae, sect. Tardae) has been described from conifer dominated forests of Pakistan. Inocybe crenata differs from other related species by having a pale-yellow pileus with crenate margins and a bumpy…
View article: Metagenomics unveils the role of hospitals and wastewater treatment plants on the environmental burden of antibiotic resistance genes and opportunistic pathogens
Metagenomics unveils the role of hospitals and wastewater treatment plants on the environmental burden of antibiotic resistance genes and opportunistic pathogens Open
Antimicrobial resistance (AMR) is a global health challenge, with hospitals and wastewater treatment plants (WWTPs) serving as significant pathways for the dissemination of antibiotic resistance genes (ARGs). This study investigates the po…
View article: Iron Oxide-Bearing Wastes as Media for Supporting Biodegradation of BTEX
Iron Oxide-Bearing Wastes as Media for Supporting Biodegradation of BTEX Open
Two common iron oxide-bearing wastes—a drinking water treatment residual and a passive mine water treatment sludge (MWTS)—were utilised with and without modification as media in microcosm experiments to treat artificial benzene, toluene, e…
View article: A peek into hospital poo: Tracking pathogens and antimicrobial resistance
A peek into hospital poo: Tracking pathogens and antimicrobial resistance Open
AIM: This study sought to track the profile of antimicrobial resistance (AMR) genes and pathogens present in hospital settings in Wales, UK, by employing wastewater-based epidemiology (WBE). BACKGROUND: Hospitals are recognized as critical…
View article: Evaluation of wastewater surveillance for SARS-CoV-2 in a prison population: a mixed-methods approach
Evaluation of wastewater surveillance for SARS-CoV-2 in a prison population: a mixed-methods approach Open
Background Prisons are high-risk settings for the transmission of communicable disease. Robust surveillance systems are required to identify and control outbreaks. Wastewater surveillance for SARS-CoV-2 was introduced in four prisons in Wa…
View article: Wastewater sample storage for physicochemical and microbiological analysis
Wastewater sample storage for physicochemical and microbiological analysis Open
Wastewater-based epidemiology (WBE) is a crucial tool for health and environmental monitoring, providing real-time data on public health indicators by analysis of sewage samples. Ensuring the integrity of these samples from collection to a…
View article: Implications of long-term sample storage on the recovery of viruses from wastewater and biobanking
Implications of long-term sample storage on the recovery of viruses from wastewater and biobanking Open
Wastewater-based monitoring has been widely implemented worldwide for the tracking of SARS-CoV-2 outbreaks and other viral diseases. In many surveillance programmes, unprocessed and processed wastewater samples are often frozen and stored …
View article: Use of wastewater from passenger ships to assess the movement of COVID-19 and other pathogenic viruses across maritime international boundaries
Use of wastewater from passenger ships to assess the movement of COVID-19 and other pathogenic viruses across maritime international boundaries Open
Objective: The worldwide spread of SARS-CoV-2 and the resulting COVID-19 pandemic has been driven by international travel. This has led to the desire to develop surveillance approaches which can estimate the rate of import of pathogenic or…
View article: Multi-factor normalisation of viral counts from wastewater improves the detection accuracy of viral disease in the community
Multi-factor normalisation of viral counts from wastewater improves the detection accuracy of viral disease in the community Open
The detection of viruses (e.g. SARS-CoV-2, norovirus) in wastewater represents an effective way to monitor the prevalence of these pathogens circulating within the community. However, accurate quantification of viral concentrations in wast…
View article: National-scale antimicrobial resistance surveillance in wastewater: A comparative analysis of HT qPCR and metagenomic approaches
National-scale antimicrobial resistance surveillance in wastewater: A comparative analysis of HT qPCR and metagenomic approaches Open
Wastewater serves as an important reservoir of antimicrobial resistance (AMR), and its surveillance can provide insights into population-level trends in AMR to inform public health policy. This study compared two common high-throughput scr…
View article: Examining the stability of viral RNA and DNA in wastewater: Effects of storage time, temperature, and freeze-thaw cycles
Examining the stability of viral RNA and DNA in wastewater: Effects of storage time, temperature, and freeze-thaw cycles Open
Wastewater-based epidemiology (WBE) has been demonstrably successful as a relatively unbiased tool for monitoring levels of SARS-CoV-2 virus circulating in communities during the COVID-19 pandemic. Accumulated biobanks of wastewater sample…
View article: Estimating Wastewater Dilution Using Chemical Markers and Incomplete Flow Measurements: Application to Normalisation of SARS-CoV-2 Measurements
Estimating Wastewater Dilution Using Chemical Markers and Incomplete Flow Measurements: Application to Normalisation of SARS-CoV-2 Measurements Open
We construct a Bayesian estimator for the dilution of a wastewater sample. When wastewater is diluted by rainwater an estimate of the level of dilution is required if we are to normalise sample measurements of disease markers such as SARS-…
View article: Simultaneous detection and characterization of common respiratory pathogens in wastewater through genomic sequencing
Simultaneous detection and characterization of common respiratory pathogens in wastewater through genomic sequencing Open
Genomic surveillance of SARS-CoV-2 has given insight into the evolution and epidemiology of the virus and its variant lineages during the COVID-19 pandemic. Expanding this approach to include a range of respiratory pathogens can better inf…
View article: Microbially mediated energy storage in the pedosphere&#160;
Microbially mediated energy storage in the pedosphere  Open
Energy storage is vital to buffer intermittency in power supplies comprised largely or wholly of variable sources (e.g. wind, solar) at large and small scale. Present technologies and those in development (e.g. electrochemical cells) have …
View article: Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland
Microbial community and antimicrobial resistance niche differentiation in a multistage, surface flow constructed wetland Open
Free-living (FL) and particulate-associated (PA) communities are distinct bacterioplankton lifestyles with different mobility and dissemination routes. Understanding spatio-temporal dynamics of PA and FL fractions will allow improvement to…
View article: Estimating Wastewater Dilution Using Chemical Markers and Incomplete Flow Measurements: Application to Normalisation of SARS-CoV-2 Measurements
Estimating Wastewater Dilution Using Chemical Markers and Incomplete Flow Measurements: Application to Normalisation of SARS-CoV-2 Measurements Open
We construct a Bayesian estimator for the dilution of a wastewater sample, and use it to estimate the prevalence of SARS-CoV-2 in Wales using data from the Wales Environmental Wastewater Analysis and Surveillance for Health project (WEWASH…
View article: Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples
Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples Open
Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this st…
View article: VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance
VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance Open
The COVID-19 pandemic demonstrated the need for rapid molecular diagnostics. Vaccination programs can provide protection and facilitate the opening of society, but newly emergent and existing viral variants capable of evading the immune sy…
View article: Monitoring SARS-CoV-2 Using Infoveillance, National Reporting Data, and Wastewater in Wales, United Kingdom: Mixed Methods Study
Monitoring SARS-CoV-2 Using Infoveillance, National Reporting Data, and Wastewater in Wales, United Kingdom: Mixed Methods Study Open
Background The COVID-19 pandemic necessitated rapid real-time surveillance of epidemiological data to advise governments and the public, but the accuracy of these data depends on myriad auxiliary assumptions, not least accurate reporting o…
View article: Toileting behaviours of the UK public: insights for reducing gender bias wastewater-based epidemiology sampling strategies
Toileting behaviours of the UK public: insights for reducing gender bias wastewater-based epidemiology sampling strategies Open
Cross-sectional survey results from a toileting behaviour survey conducted between the 27th to the 28th of June 2022. Participants (n = 2109) were aged 18 years or older and were living in the UK. The survey consisted of 17 closed-ended qu…
View article: Toileting behaviours of the UK public: insights for reducing gender bias wastewater-based epidemiology sampling strategies
Toileting behaviours of the UK public: insights for reducing gender bias wastewater-based epidemiology sampling strategies Open
Cross-sectional survey results from a toileting behaviour survey conducted between the 27th to the 28th of June 2022. Participants (n = 2109) were aged 18 years or older and were living in the UK. The survey consisted of 17 closed-ended qu…