Jon Cuccui
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View article: Immunological and pathobiological characteristics of a novel live Salmonella Typhimurium-vectored Campylobacter vaccine candidate for layer chickens
Immunological and pathobiological characteristics of a novel live Salmonella Typhimurium-vectored Campylobacter vaccine candidate for layer chickens Open
Introduction Spotty liver disease (SLD) poses a significant economic and animal welfare threat to the global cage-free egg industry, primarily due to infection by the emerging pathogen Campylobacter hepaticus . SLD can lead to a significan…
Investigation into the efficiency of diverse <i>N</i> ‐linking oligosaccharyltransferases for glycoengineering using a standardised cell‐free assay Open
The application of bacterial oligosaccharyltransferases (OSTs) such as the Campylobacter jejuni PglB for glycoengineering has attracted considerable interest in glycoengineering and glycoconjugate vaccine development. However, PglB has lim…
View article: Evaluation of a FlpA Glycoconjugate Vaccine with Ten N-Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens
Evaluation of a FlpA Glycoconjugate Vaccine with Ten N-Heptasaccharide Glycan Moieties to reduce Campylobacter jejuni Colonisation in Chickens Open
Campylobacter is a major cause of acute gastroenteritis in humans, and infections can be followed by inflammatory neuropathies and other sequelae. Handling or consumption of poultry meat is the primary risk factor for human campylobacterio…
Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS) Open
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Ins…
Development of a novel glycoengineering platform for the rapid production of conjugate vaccines Open
Antimicrobial resistance (AMR) is threatening the lives of millions worldwide. Antibiotics which once saved countless lives, are now failing, ushering in vaccines development as a current global imperative. Conjugate vaccines produced eith…
Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction Open
The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differenc…
View article: Additional file 2 of Multivalent poultry vaccine development using Protein Glycan Coupling Technology
Additional file 2 of Multivalent poultry vaccine development using Protein Glycan Coupling Technology Open
Additional file 2: WGS of χ7122 and χ7122 pgl summary
Characterization of Posttranslationally Modified Multidrug Efflux Pumps Reveals an Unexpected Link between Glycosylation and Antimicrobial Resistance Open
Nearly all bacterial species have at least a single glycosylation system, but the direct effects of these posttranslational protein modifications are unresolved. Glycoproteome-wide analysis of several bacterial pathogens has revealed gener…
Evaluation of Glycosylated FlpA and SodB as Subunit Vaccines Against Campylobacter jejuni Colonisation in Chickens Open
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide and the handling or consumption of contaminated poultry meat is the key source of infection. C. jejuni proteins FlpA and SodB and glycoconjugates contai…
Virulence of the Emerging Pathogen, <i>Burkholderia Pseudomallei</i> , Depends Upon the <i>O</i> -linked Oligosaccharyltransferase, PglL Open
Aim: We sought to characterize the contribution of the O-OTase, PglL, to virulence in two Burkholderia spp. by comparing isogenic mutants in Burkholderia pseudomallei with the related species, Burkholderia thailandensis. Materials & method…
Ferric Citrate Regulator FecR Is Translocated across the Bacterial Inner Membrane via a Unique Twin-Arginine Transport-Dependent Mechanism Open
Iron is essential for nearly all living organisms due to its role in metabolic processes and as a cofactor for many enzymes. The FecRI signal transduction pathway regulates citrate-mediated iron import in many Gram-negative bacteria, inclu…
Quantitative Analyses Reveal Novel Roles for <i>N-</i> Glycosylation in a Major Enteric Bacterial Pathogen Open
Advances in genomics and mass spectrometry have revealed several types of glycosylation systems in bacteria. However, why bacterial proteins are modified remains poorly defined. Here, we investigated the role of general N- linked glycosyla…
<i>N</i> -linked glycans confer protein stability and modulate multidrug efflux pump assembly <i>in Campylobacter jejuni</i> Open
It is now apparent that nearly all bacteria species have at least a single glycosylation system, but the direct effect(s) of these protein post translational modifications are unresolved. In this study, we used the general N -linked glycos…
View article: Additional file 1: of Cytoplasmic glycoengineering of Apx toxin fragments in the development of Actinobacillus pleuropneumoniae glycoconjugate vaccines
Additional file 1: of Cytoplasmic glycoengineering of Apx toxin fragments in the development of Actinobacillus pleuropneumoniae glycoconjugate vaccines Open
Table S1. Full LC-MS/MS output. (XLSX 15 kb)
An O-Antigen Glycoconjugate Vaccine Produced Using Protein Glycan Coupling Technology Is Protective in an Inhalational Rat Model of Tularemia Open
There is a requirement for an efficacious vaccine to protect people against infection from Francisella tularensis , the etiological agent of tularemia. The lipopolysaccharide (LPS) of F. tularensis is suboptimally protective against a pare…
Inactivation of bpsl1039-1040 ATP-binding cassette transporter reduces intracellular survival in macrophages, biofilm formation and virulence in the murine model of Burkholderia pseudomallei infection Open
Burkholderia pseudomallei, a gram-negative intracellular bacillus, is the causative agent of a tropical infectious disease called melioidosis. Bacterial ATP-binding cassette (ABC) transporters import and export a variety of molecules acros…
The <i>N</i> -linking glycosylation system from <i>Actinobacillus pleuropneumoniae</i> is required for adhesion and has potential use in glycoengineering Open
Actinobacillus pleuropneumoniae is a mucosal respiratory pathogen causing contagious porcine pleuropneumonia. Pathogenesis studies have demonstrated a major role for the capsule, exotoxins and outer membrane proteins. Actinobacillus pleuro…
Glycosylation system investigation, Terra et al. Table S1 ;Glycosylation system investigation, Terra et al. Table S2;Glycosylation system investigation, Terra et al. Figure S1;Glycosylation system investigation, Terra et al. Figure S2 from The <i>N</i>-linking glycosylation system from <i>Actinobacillus pleuropneumoniae</i> is required for adhesion and has potential use in glycoengineering Open
Strains and plasmids used in this study; Primers used in this study for reverse transcriptase analysis of RNA extracts from A. pleuropneumoniae HS143, complementation of ngt and agt mutants and construction of A. pleuropneumoniae deletion …
Recombinant expression of<i>Streptococcus pneumoniae</i>capsular polysaccharides in<i>Escherichia coli</i> Open
Currently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children. The major determinant for pathogenesis is a polysaccharide capsule that…