Jon E. Levine
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View article: Hypothalamic Estrogen Receptor α Is Essential for Female Marmoset Sexual Behavior Without Protecting From Obesity
Hypothalamic Estrogen Receptor α Is Essential for Female Marmoset Sexual Behavior Without Protecting From Obesity Open
Context Estrogen receptor α (ERα) in the ventromedial (VMN) and arcuate (ARC) nuclei of female rodent mediobasal hypothalami (MBHs) provides a crucial molecular gateway facilitating estradiol (E2) regulation of sexual behavior, reproductiv…
View article: Proceedings of the 15 <sup>th</sup> International Newborn Brain Conference: Neuroprotection strategies in the neonate
Proceedings of the 15 <sup>th</sup> International Newborn Brain Conference: Neuroprotection strategies in the neonate Open
BACKGROUND: Cerebral intraventricular hemorrhage (IVH) is a major cause of neurodevelopmental impairment in preterm infants.IVH is characterized by vessel rupture and a rapid accumulation of blood within the ventricles.Subsequent hemolysis…
View article: Whole Genome Analysis of SNV and Indel Polymorphism in Common Marmosets (Callithrix jacchus)
Whole Genome Analysis of SNV and Indel Polymorphism in Common Marmosets (Callithrix jacchus) Open
The common marmoset (Callithrix jacchus) is one of the most widely used nonhuman primate models of human disease. Owing to limitations in sequencing technology, early genome assemblies of this species using short-read sequencing suffered f…
View article: FRI276 KNDy-Neuron Kisspeptin Is Dispensable For Feeding Behavior, But Critical For Energy Expenditure Leading To Metabolic Dysfunction In Female Mice
FRI276 KNDy-Neuron Kisspeptin Is Dispensable For Feeding Behavior, But Critical For Energy Expenditure Leading To Metabolic Dysfunction In Female Mice Open
Disclosure: N. Nandankar: None. H. Ganesh: None. A.L. Negron: None. S. Al-Samerria: None. J.E. Levine: None. S. Radovick: None. Hypothalamic kisspeptin (Kiss1) is a critical master regulator of the hypothalamic-pituitary-gonadal axis respo…
View article: TrkB-mediated sustained neuroprotection is sex-specific and ERα dependent in adult mice following neonatal hypoxia ischemia
TrkB-mediated sustained neuroprotection is sex-specific and ERα dependent in adult mice following neonatal hypoxia ischemia Open
Background Neonatal hypoxia ischemia (HI) related brain injury is one of the major causes of life-long neurological morbidities that result in learning and memory impairments. Evidence suggests that male neonates are more susceptible to th…
View article: Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors
Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors Open
Introduction: Targeted gene editing is proposed as a therapeutic approach for numerous disorders, including neurological diseases. As the brain is organized into neural networks, it is critical to understand how anatomically connected stru…
View article: Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors
Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors Open
Introduction Targeted gene editing is proposed as a therapeutic approach for numerous disorders, including neurological diseases. As the brain is organized into neural networks, it is critical to understand how anatomically connected struc…
View article: Multimodal analyses reveal genes driving electrophysiological maturation of neurons in the primate prefrontal cortex
Multimodal analyses reveal genes driving electrophysiological maturation of neurons in the primate prefrontal cortex Open
SUMMARY The prefrontal cortex (PFC) is critical for myriad high-cognitive functions and is associated with several neuropsychiatric disorders. Here, using Patch-seq and single-nucleus multiomic analyses, we identified genes and regulatory …
View article: Efficient in vivo neuronal genome editing in the mouse brain using nanocapsules containing CRISPR-Cas9 ribonucleoproteins
Efficient in vivo neuronal genome editing in the mouse brain using nanocapsules containing CRISPR-Cas9 ribonucleoproteins Open
Genome editing of somatic cells via clustered regularly interspaced short palindromic repeats (CRISPR) offers promise for new therapeutics to treat a variety of genetic disorders, including neurological diseases. However, the dense and com…
View article: Aromatase Inhibition Eliminates Sexual Receptivity Without Enhancing Weight Gain in Ovariectomized Marmoset Monkeys
Aromatase Inhibition Eliminates Sexual Receptivity Without Enhancing Weight Gain in Ovariectomized Marmoset Monkeys Open
Context Ovarian estradiol supports female sexual behavior and metabolic function. While ovariectomy (OVX) in rodents abolishes sexual behavior and enables obesity, OVX in nonhuman primates decreases, but does not abolish, sexual behavior, …
View article: Novel Injury Scoring Tool for Assessing Brain Injury following Neonatal Hypoxia-Ischemia in Mice
Novel Injury Scoring Tool for Assessing Brain Injury following Neonatal Hypoxia-Ischemia in Mice Open
The variability of severity in hypoxia-ischemia (HI)-induced brain injury among research subjects is a major challenge in developmental brain injury research. Our laboratory developed a novel injury scoring tool based on our gross patholog…
View article: Deficiency of arcuate nucleus kisspeptin results in postpubertal central hypogonadism
Deficiency of arcuate nucleus kisspeptin results in postpubertal central hypogonadism Open
We demonstrate through a novel, conditional knockout mouse model of arcuate nucleus (ARC)-specific kisspeptin in the KNDy neuron that ARC kisspeptin is critical for estrous cyclicity in female mice and GnRH/LH pulsatility in both sexes. Ou…
View article: Development of a Novel Tetracycline-Inducible <i>Kiss1</i>-Cre Mouse Line for Temporally Controlled Gene Deletion in Kisspeptin Neurons
Development of a Novel Tetracycline-Inducible <i>Kiss1</i>-Cre Mouse Line for Temporally Controlled Gene Deletion in Kisspeptin Neurons Open
The tetracycline (Tet)-controlled inducible system is the most widely used reversible system for transgenic expression in mice. Previously, we generated a GnRH-CreTeR mouse model, using a first-generation Tet-inducible system to temporally…
View article: Mice With Targeted Deletion of ARC Kisspeptin Exhibit Immature Gametogenesis and Impaired Fertility
Mice With Targeted Deletion of ARC Kisspeptin Exhibit Immature Gametogenesis and Impaired Fertility Open
Hypothalamic kisspeptin is primarily synthesized in two discrete nuclei - the anteroventral periventricular (AVPV) and the arcuate (ARC) nuclei. We have previously developed a selective, conditional ARC kisspeptin knock-out (KO) mouse line…
View article: The NIH Somatic Cell Genome Editing program
The NIH Somatic Cell Genome Editing program Open
The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to accelerate the development of s…
View article: Sequence diversity analyses of an improved rhesus macaque genome enhance its biomedical utility
Sequence diversity analyses of an improved rhesus macaque genome enhance its biomedical utility Open
A high-quality rhesus macaque genome Genome technology has improved substantially since the first full organismal genomes were generated. Applying new technology, Warren et al. refined the genome of the rhesus macaque, a model nonhuman pri…
View article: Brain Aromatase and the Regulation of Sexual Activity in Male Mice
Brain Aromatase and the Regulation of Sexual Activity in Male Mice Open
The biologically active estrogen estradiol has important roles in adult brain physiology and sexual behavior. A single gene, Cyp19a1, encodes aromatase, the enzyme that catalyzes the conversion of testosterone to estradiol in the testis an…
View article: SUN-243 Deletion of KNDy Neuron-Specific KISS1 Disrupts Estrous Cyclicity and LH Pulsatility in Female Mice
SUN-243 Deletion of KNDy Neuron-Specific KISS1 Disrupts Estrous Cyclicity and LH Pulsatility in Female Mice Open
Kisspeptin (encoded by Kiss1), a neuropeptide critically involved in neuroendocrine regulation of reproduction, is primarily synthesized in two discrete hypothalamic nuclei: the anteroventral periventricular area (AVPV) and arcuate nucleus…
View article: SAT-597 Hypothalamic ESR1 Gene Knockdown Elicits Intermittent Decrement in Postprandial Energy Expenditure Associated with Obesity Onset in Female Rhesus Monkeys
SAT-597 Hypothalamic ESR1 Gene Knockdown Elicits Intermittent Decrement in Postprandial Energy Expenditure Associated with Obesity Onset in Female Rhesus Monkeys Open
Declining serum estradiol (E2) levels during the menopausal transition are associated with increased central adiposity and heightened risk for metabolic disease. Estrogenic effects on adiposity and metabolism in female rodents are primaril…
View article: OR09-03 Brain Aromatase Is Essential for Regulation of Sexual Activity in Male Mice
OR09-03 Brain Aromatase Is Essential for Regulation of Sexual Activity in Male Mice Open
Introduction: The biologically active form of estrogen, estradiol (E2), has important organizational roles in brain development and activational roles in adult brain physiology and behavior. It has been proposed that E2 formation in the br…
View article: SAT-587 Molecular Markers of Beige Adipose Following ESR1 Knockdown in the Mediobasal Hypothalamus of Adult Female Rhesus Monkeys
SAT-587 Molecular Markers of Beige Adipose Following ESR1 Knockdown in the Mediobasal Hypothalamus of Adult Female Rhesus Monkeys Open
Our studies in female marmoset monkeys show that the ablation of ovarian estradiol (E2) production fails to alter energy homeostasis or body fat accumulation. Peripheral E2 may therefore not play a crucial role in metabolic regulation in f…
View article: OR31-05 Emergence of Ovarian Hyperandrogenism and Luteal Insufficiency Following ESR1 Knockdown in the Mediobasal Hypothalamus of Adult Female Rhesus Monkeys
OR31-05 Emergence of Ovarian Hyperandrogenism and Luteal Insufficiency Following ESR1 Knockdown in the Mediobasal Hypothalamus of Adult Female Rhesus Monkeys Open
Diminished estradiol (E2) negative feedback action by neuronal ESR1 in the arcuate nucleus (ARC) of the mediobasal hypothalamus (MBH) is hypothesized to cause gonadotropin-releasing hormone (GnRH) hypersecretion, and thus LH excess, contri…
View article: Naturally Occurring and Experimentally Induced Rhesus Macaque Models for Polycystic Ovary Syndrome: Translational Gateways to Clinical Application
Naturally Occurring and Experimentally Induced Rhesus Macaque Models for Polycystic Ovary Syndrome: Translational Gateways to Clinical Application Open
Indian rhesus macaque nonhuman primate models for polycystic ovary syndrome (PCOS) implicate both female hyperandrogenism and developmental molecular origins as core components of PCOS etiopathogenesis. Establishing and exploiting macaque …