Jon P. Boyle
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View article: The roles of the <i>Listeria monocytogenes</i> post-translocation chaperones PrsA1 and PrsA2 in protein secretion and stress resistance
The roles of the <i>Listeria monocytogenes</i> post-translocation chaperones PrsA1 and PrsA2 in protein secretion and stress resistance Open
PrsA1 and PrsA2 are parvulin peptidyl-prolyl isomerases that function as post-translocation secretion chaperones in Listeria monocytogenes . To assess the contributions of PrsA1 and PrsA2 to overall L. monocytogenes protein secretion, we a…
View article: <i>Toxoplasma gondii</i> and the Brain: Frenemies? Or Just Outright Foes?
<i>Toxoplasma gondii</i> and the Brain: Frenemies? Or Just Outright Foes? Open
Toxoplasma gondii is an apicomplexan parasite with an enormous global reach, infecting over a billion people worldwide. An opportunist in humans, T. gondii causes severe disease only in a select few scenarios but is otherwise relatively be…
View article: Mouse innate resistance to <i>Neospora caninum</i> infection is driven by early production of IFNγ by NK cells in response to parasite ligands
Mouse innate resistance to <i>Neospora caninum</i> infection is driven by early production of IFNγ by NK cells in response to parasite ligands Open
Toxoplasma gondii is capable of being transmitted by nearly all warm-blooded animals, and rodents are a major source of parasite dissemination, yet mechanisms driving its broad host range are poorly understood. Although a phylogenetically …
View article: <i>Toxoplasma gondii</i>infection misdirects placental trophoblast lineage specification
<i>Toxoplasma gondii</i>infection misdirects placental trophoblast lineage specification Open
Pregnancy is a critical point of vulnerability to infection and other insults that could compromise proper fetal development. The placenta acts as a protective and nutrient-permeable barrier to most infectious agents, but a few are capable…
View article: ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii
ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii Open
The apicomplexan intracellular parasite Toxoplasma gondii is a major food borne pathogen that is highly prevalent in the global population. The majority of the T . gondii proteome remains uncharacterized and the organization of proteins in…
View article: The trophoblast surface becomes refractory to adhesion by congenitally transmitted <i>Toxoplasma gondii</i> and <i>Listeria monocytogenes</i> during cytotrophoblast to syncytiotrophoblast development
The trophoblast surface becomes refractory to adhesion by congenitally transmitted <i>Toxoplasma gondii</i> and <i>Listeria monocytogenes</i> during cytotrophoblast to syncytiotrophoblast development Open
The placenta is a critical barrier against viral, bacterial, and eukaryotic pathogens. For most teratogenic pathogens, the precise molecular mechanisms of placental resistance are still being unraveled. Given the importance of understandin…
View article: Toxoplasma gondii mitochondrial association factor 1b interactome reveals novel binding partners including Ral GTPase accelerating protein α1
Toxoplasma gondii mitochondrial association factor 1b interactome reveals novel binding partners including Ral GTPase accelerating protein α1 Open
The intracellular parasite, Toxoplasma gondii, has developed sophisticated molecular strategies to subvert host processes and promote growth and survival. During infection, T. gondii replicates in a parasitophorous vacuole (PV) and modulat…
View article: Human trophoblast stem cells can be used to model placental susceptibility to<i>Toxoplasma gondii</i>and highlight the critical importance of the trophoblast cell surface in pathogen resistance
Human trophoblast stem cells can be used to model placental susceptibility to<i>Toxoplasma gondii</i>and highlight the critical importance of the trophoblast cell surface in pathogen resistance Open
The placenta is a critical barrier against viral, bacterial, and eukaryotic pathogens. For most teratogenic pathogens, the precise molecular mechanisms of placental resistance are still being unraveled. Given the importance to understand t…
View article: A transcriptional network required for bradyzoite development in Toxoplasma gondii is dispensable for recrudescent disease
A transcriptional network required for bradyzoite development in Toxoplasma gondii is dispensable for recrudescent disease Open
Identification of regulators of Toxoplasma gondii bradyzoite development and cyst formation is the most direct way to address the importance of parasite development in long-term persistence and reactivation of this parasite. Here we show t…
View article: A transcriptional network required for <i>Toxoplasma gondii</i> tissue cyst formation is dispensable for long-term persistence
A transcriptional network required for <i>Toxoplasma gondii</i> tissue cyst formation is dispensable for long-term persistence Open
Cyst formation is a key feature of the T. gondii life cycle but the genetic networks that drive this process are not yet fully characterized. To identify new components of this network, we compared T. gondii to its nearest extant relative …
View article: ToxoNet: A high confidence map of protein-protein interactions in<i>Toxoplasma gondii</i>reveals novel virulence factors implicated in host cell invasion
ToxoNet: A high confidence map of protein-protein interactions in<i>Toxoplasma gondii</i>reveals novel virulence factors implicated in host cell invasion Open
The apicomplexan intracellular parasite Toxoplasma gondii is a major food borne pathogen with significant impact in children and during pregnancy. The majority of the T. gondii proteome remains uncharacterized and the organization of prote…
View article: The<i>Toxoplasma</i>Polymorphic Effector GRA15 Mediates Seizure Induction by Modulating Interleukin-1 Signaling in the Brain
The<i>Toxoplasma</i>Polymorphic Effector GRA15 Mediates Seizure Induction by Modulating Interleukin-1 Signaling in the Brain Open
Inflammation in the brain caused by infections lead to seizures and other neurological symptoms. But the microbial products that induce seizures as well as the host pathways downstream of these factors are largely unknown.
View article: Third-generation sequencing revises the molecular karyotype for <i>Toxoplasma gondii</i> and identifies emerging copy number variants in sexual recombinants
Third-generation sequencing revises the molecular karyotype for <i>Toxoplasma gondii</i> and identifies emerging copy number variants in sexual recombinants Open
Toxoplasma gondii is a useful model for intracellular parasitism given its ease of culture in the laboratory and genomic resources. However, as for many other eukaryotes, the T. gondii genome contains hundreds of sequence gaps owing to rep…
View article: <i>Toxoplasma gondii</i> association with host mitochondria requires key mitochondrial protein import machinery
<i>Toxoplasma gondii</i> association with host mitochondria requires key mitochondrial protein import machinery Open
Significance Many pathogens manipulate the host environment to promote their survival and eventual transmission. Toxoplasma gondii , an intracellular parasite that can cause disease in those with weakened immune systems, is no exception. O…
View article: Toxoplasma gondii All_vs_All_Correlation_Matrix and related
Toxoplasma gondii All_vs_All_Correlation_Matrix and related Open
Dataset generated by performing an all-v-all correlation analysis in R using the "cor" function. Input data were normalized microarray data from multiple Toxoplasma gondii strains and life stages.Files are all tab-delimited:AllvAllCorrel.t…
View article: A Comparison of Stage Conversion in the Coccidian Apicomplexans Toxoplasma gondii, Hammondia hammondi, and Neospora caninum
A Comparison of Stage Conversion in the Coccidian Apicomplexans Toxoplasma gondii, Hammondia hammondi, and Neospora caninum Open
Stage conversion is a critical life cycle feature for several Apicomplexan parasites as the ability to switch between life forms is critical for replication, dissemination, pathogenesis and ultimately, transmission to a new host. In order …
View article: Transcriptional Profiling Suggests T Cells Cluster around Neurons Injected with Toxoplasma gondii Proteins
Transcriptional Profiling Suggests T Cells Cluster around Neurons Injected with Toxoplasma gondii Proteins Open
Like other persistent intracellular pathogens, Toxoplasma gondii , a protozoan parasite, has evolved to evade the immune system and establish a chronic infection in specific cells and organs, including neurons in the CNS. Understanding T. …
View article: Strain-specific disruption of interferon-stimulated N-myc and STAT interactor (NMI) function by <i>Toxoplasma gondii</i> type I ROP18 in human cells
Strain-specific disruption of interferon-stimulated N-myc and STAT interactor (NMI) function by <i>Toxoplasma gondii</i> type I ROP18 in human cells Open
Toxoplasma gondii rhoptry protein Tg ROP18 is a polymorphic virulence effector that targets immunity-related GTPases (IRGs) in rodents. Given that IRGs are uniquely diversified in rodents and not in other T. gondii intermediate hosts, the …
View article: Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions
Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions Open
Toxoplasma gondii and Hammondia hammondi are closely-related coccidian intracellular parasites that differ in their ability to cause disease in animal and (likely) humans. The role of the host response in these phenotypic differences is no…
View article: Bystander cells lack any detectable expression of parasite transcript.
Bystander cells lack any detectable expression of parasite transcript. Open
CT values of H. hammondi GRA1 transcript detection in infected and bystander THP-1 cells cells (H. hammondi added to cells in the Transwell insert) N.D., Not Detected. (PDF)
View article: Transcriptional profiling reveals T cells cluster around neurons injected with<i>Toxoplasma gondii</i>proteins
Transcriptional profiling reveals T cells cluster around neurons injected with<i>Toxoplasma gondii</i>proteins Open
Toxoplasma gondii ’s tropism for and persistence in the CNS underlies the symptomatic disease Toxoplasma causes in humans. Our recent work has shown that neurons are the primary CNS cell with which Toxoplasma interacts and infects in vivo …
View article: Third generation sequencing revises the molecular karyotype for<i>Toxoplasma gondii</i>and identifies emerging copy number variants in sexual recombinants
Third generation sequencing revises the molecular karyotype for<i>Toxoplasma gondii</i>and identifies emerging copy number variants in sexual recombinants Open
Toxoplasma gondii is an obligate intracellular parasite that has a significant impact on human health, especially in the immunocompromised. This parasite is also a useful genetic model for intracellular parasitism given its ease of culture…
View article: Immediate Interferon Gamma Induction Determines Murine Host Compatibility Differences between Toxoplasma gondii and Neospora caninum
Immediate Interferon Gamma Induction Determines Murine Host Compatibility Differences between Toxoplasma gondii and Neospora caninum Open
Rodents are critical for the transmission of Toxoplasma gondii to the definitive feline host via predation, and this relationship has been extensively studied as a model for immune responses to parasites. Neospora caninum is a closely rela…
View article: Impact of Engineered Expression of Mitochondrial Association Factor 1b on<i>Toxoplasma gondii</i>Infection and the Host Response in a Mouse Model
Impact of Engineered Expression of Mitochondrial Association Factor 1b on<i>Toxoplasma gondii</i>Infection and the Host Response in a Mouse Model Open
The parasite Toxoplasma gondii currently infects approximately one-third of the world’s population and causes life-threatening toxoplasmosis in individuals with undeveloped or weakened immune systems. Current treatments are unable to cure …
View article: Dissection of the in vitro developmental program of Hammondia hammondi reveals a link between stress sensitivity and life cycle flexibility in Toxoplasma gondii
Dissection of the in vitro developmental program of Hammondia hammondi reveals a link between stress sensitivity and life cycle flexibility in Toxoplasma gondii Open
Most eukaryotic parasites are obligately heteroxenous, requiring sequential infection of different host species in order to survive. Toxoplasma gondii is a rare exception to this rule, having a uniquely facultative heteroxenous life cycle.…