Jonas V. Schaefer
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View article: Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma
Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma Open
The embryonic transcription factor TBXT (brachyury) drives chordoma, a spinal neoplasm without effective drug therapies. TBXT’s regulatory network is poorly understood, and strategies to disrupt its activity for therapeutic purposes are la…
View article: A nucleotide‐independent, pan‐ <scp>RAS</scp> ‐targeted <scp>DARPin</scp> elicits anti‐tumor activity in a multimodal manner
A nucleotide‐independent, pan‐ <span>RAS</span> ‐targeted <span>DARPin</span> elicits anti‐tumor activity in a multimodal manner Open
The KRAS oncoprotein is a frequent tumor driver in lung, pancreatic, and colorectal cancers and has proven to be a challenging pharmaceutical target. The first KRAS‐targeted therapeutics are now being tested in clinical trials but the cons…
View article: Custom affinity probes reveal DNA-damage-induced, ssDNA-independent chromatin SUMOylation in budding yeast
Custom affinity probes reveal DNA-damage-induced, ssDNA-independent chromatin SUMOylation in budding yeast Open
The small ubiquitin-related modifier SUMO regulates cellular processes in eukaryotes either by modulating individual protein-protein interactions or with relaxed substrate selectivity by group modification. Here, we report the isolation an…
View article: DARPins as a novel tool to detect and degrade p73
DARPins as a novel tool to detect and degrade p73 Open
The concept of Targeted Protein Degradation (TPD) has been introduced as an attractive alternative to the development of classical inhibitors. TPD can extend the range of proteins that can be pharmacologically targeted beyond the classical…
View article: Broadening the Utility of Farnesyltransferase-Catalyzed Protein Labeling Using Norbornene–Tetrazine Click Chemistry
Broadening the Utility of Farnesyltransferase-Catalyzed Protein Labeling Using Norbornene–Tetrazine Click Chemistry Open
Bioorthogonal chemistry has gained widespread use in the study of many biological systems of interest, including protein prenylation. Prenylation is a post-translational modification, in which one or two 15- or 20-carbon isoprenoid chains …
View article: Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells
Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells Open
The orchestrated assembly of actin and actin-binding proteins into cytoskeletal structures coordinates cell morphology changes during migration, cytokinesis, and adaptation to external stimuli. The accurate and unbiased visualization of th…
View article: CCDC 2069871: Experimental Crystal Structure Determination
CCDC 2069871: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Diversity-oriented synthesis encoded by deoxyoligonucleotides
Diversity-oriented synthesis encoded by deoxyoligonucleotides Open
Diversity-oriented synthesis (DOS) is a powerful strategy to prepare molecules with underrepresented features in commercial screening collections, resulting in the elucidation of novel biological mechanisms. In parallel to the development …
View article: A DARPin Increases the Catalytic Activity of Botulinum Neurotoxin A1
A DARPin Increases the Catalytic Activity of Botulinum Neurotoxin A1 Open
In this study, we characterized Designed Ankyrin Repat Proteins (DARPins) as investigative tools to probe botulinum neurotoxin (BoNT) function and as potential antidotes for botulism. We selected DARPins against the catalytic domain of BoN…
View article: Diversity-oriented synthesis encoded by deoxyoligonucleotides
Diversity-oriented synthesis encoded by deoxyoligonucleotides Open
Diversity-oriented synthesis (DOS)is a powerful strategy to prepare molecules with underrepresented features in commercial screening collections, resulting in the elucidation of novel biological mechanisms. In parallel to the development o…
View article: Enzymatic Construction of DARPin-Based Targeted Delivery Systems Using Protein Farnesyltransferase and a Capture and Release Strategy
Enzymatic Construction of DARPin-Based Targeted Delivery Systems Using Protein Farnesyltransferase and a Capture and Release Strategy Open
Protein-based conjugates have been extensively utilized in various biotechnological and therapeutic applications. In order to prepare homogeneous conjugates, site-specific modification methods and efficient purification strategies are both…
View article: Nuclear myosin VI maintains replication fork stability
Nuclear myosin VI maintains replication fork stability Open
The actin cytoskeleton is of fundamental importance for cellular structure and plasticity. However, abundance and function of filamentous (F-) actin in the nucleus are still controversial. Here we show that the actin-based molecular motor …
View article: Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways
Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways Open
The deacetylase HDAC6 has tandem catalytic domains and a zinc finger domain (ZnF) binding ubiquitin (Ub). While the catalytic domain has an antiviral effect, the ZnF facilitates influenza A virus (IAV) infection and cellular stress respons…
View article: Structural basis of adenylyl cyclase 9 activation
Structural basis of adenylyl cyclase 9 activation Open
Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is autoinhibited by the AC9 C-terminus. Although our recent stru…
View article: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc
Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc Open
Summary How morphogen gradients control patterning and growth in developing tissues remains largely unknown due to lack of tools manipulating morphogen gradients. Here, we generate two membrane-tethered protein binders that manipulate diff…
View article: Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome
Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome Open
The Chip/LIM-domain binding protein (LDB)–single-stranded DNA-binding protein (SSDP) (ChiLS) complex controls numerous cell-fate decisions in animal cells, by mediating transcription of developmental control genes via remote enhancers. Chi…
View article: Encoded Library Technologies as Integrated Lead Finding Platforms for Drug Discovery
Encoded Library Technologies as Integrated Lead Finding Platforms for Drug Discovery Open
The scope of targets investigated in pharmaceutical research is continuously moving into uncharted territory. Consequently, finding suitable chemical matter with current compound collections is proving increasingly difficult. Encoded libra…
View article: Correction: DARPins recognizing mTFP1 as novel reagents for <i>in vitro</i> and <i>in vivo</i> protein manipulations (doi:10.1242/bio.036749)
Correction: DARPins recognizing mTFP1 as novel reagents for <i>in vitro</i> and <i>in vivo</i> protein manipulations (doi:10.1242/bio.036749) Open
There were errors published in Biology Open (2018) 11, bio036749 (doi:10.1242/bio.036749).The article title originally used the word ‘recognize’, where the word used should have been ‘recognizing’.The correct title appears above and both t…