Jonathan G. Van Vranken
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View article: SUN-647 RBM43 Controls PGC1α Translation and a PGC1α-STING Signaling Axis
SUN-647 RBM43 Controls PGC1α Translation and a PGC1α-STING Signaling Axis Open
Disclosure: P.A. Dumesic: None. S.E. Wilensky: None. S. Bose: None. J.G. Van Vranken: None. S.P. Gygi: None. B. Spiegelman: None. Obesity is associated with systemic inflammation that impairs mitochondrial function. In adipose tissue, pro-…
View article: On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions
On-target toxicity limits the efficacy of CDK11 inhibition against cancers with 1p36 deletions Open
The cyclin-dependent kinase CDK11 is an understudied kinase that has been the subject of conflicting reports regarding its function in cancer. Here, we combine genetic and pharmacological approaches to demonstrate that CDK11 is a critical …
View article: A dual-purification system to isolate mitochondrial subpopulations
A dual-purification system to isolate mitochondrial subpopulations Open
Mitochondria perform diverse functions, including producing ATP through oxidative phosphorylation, synthesizing macromolecule precursors, maintaining redox balance among many others. Given this diversity of functions, we and others have hy…
View article: Author response: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Author response: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
View article: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to approximate compound-dependent chang…
View article: Author response: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Author response: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
View article: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to approximate compound-dependent chang…
View article: Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST
Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST Open
Ergothioneine (EGT) is a diet-derived, atypical amino acid that accumulates to high levels in human tissues. Reduced EGT levels have been linked to age-related disorders, including neurodegenerative and cardiovascular diseases, while EGT s…
View article: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to approximate compound-dependent chang…
View article: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent chan…
View article: Mustn1 is a smooth muscle cell-secreted microprotein that modulates skeletal muscle extracellular matrix composition
Mustn1 is a smooth muscle cell-secreted microprotein that modulates skeletal muscle extracellular matrix composition Open
View article: Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays
Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays Open
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to approximate compound-dependent chang…
View article: Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms
Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms Open
Mitochondrial membrane potential directly powers many critical functions of mitochondria, including ATP production, mitochondrial protein import, and metabolite transport. Its loss is a cardinal feature of aging and mitochondrial diseases,…
View article: Author response: Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms
Author response: Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Mitocho…
View article: Insulin sensitization by small molecules enhancing GLUT4 translocation
Insulin sensitization by small molecules enhancing GLUT4 translocation Open
View article: Isolation of extracellular fluids reveals novel secreted bioactive proteins from muscle and fat tissues
Isolation of extracellular fluids reveals novel secreted bioactive proteins from muscle and fat tissues Open
View article: RBM43 links adipose inflammation and energy expenditure through translational regulation of PGC1α
RBM43 links adipose inflammation and energy expenditure through translational regulation of PGC1α Open
Adipose thermogenesis involves specialized mitochondrial function that counteracts metabolic disease through dissipation of chemical energy as heat. However, inflammation present in obese adipose tissue can impair oxidative metabolism. Her…
View article: A proteome-wide atlas of drug mechanism of action
A proteome-wide atlas of drug mechanism of action Open
View article: Phosphate Starvation Signaling Increases Mitochondrial Membrane Potential through Respiration-independent Mechanisms
Phosphate Starvation Signaling Increases Mitochondrial Membrane Potential through Respiration-independent Mechanisms Open
Mitochondrial membrane potential directly powers many critical functions of mitochondria, including ATP production, mitochondrial protein import, and metabolite transport. Its loss is a cardinal feature of aging and mitochondrial diseases,…
View article: Amelioration of pathologic α-synuclein-induced Parkinson’s disease by irisin
Amelioration of pathologic α-synuclein-induced Parkinson’s disease by irisin Open
Physical activity provides clinical benefit in Parkinson’s disease (PD). Irisin is an exercise-induced polypeptide secreted by skeletal muscle that crosses the blood–brain barrier and mediates certain effects of exercise. Here, we show tha…
View article: Insulin Sensitization by Small Molecules Enhancing GLUT4 Translocation
Insulin Sensitization by Small Molecules Enhancing GLUT4 Translocation Open
View article: Assessing target engagement using proteome-wide solvent shift assays
Assessing target engagement using proteome-wide solvent shift assays Open
Recent advances in mass spectrometry (MS) have enabled quantitative proteomics to become a powerful tool in the field of drug discovery, especially when applied toward proteome-wide target engagement studies. Similar to temperature gradien…
View article: Author response: Assessing target engagement using proteome-wide solvent shift assays
Author response: Assessing target engagement using proteome-wide solvent shift assays Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Recent advances in mas…
View article: Assessing target engagement using proteome-wide solvent shift assays
Assessing target engagement using proteome-wide solvent shift assays Open
Recent advances in mass spectrometry (MS) have enabled quantitative proteomics to become a powerful tool in the field of drug discovery, especially when applied toward proteome-wide target engagement studies. Similar to temperature gradien…
View article: Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay
Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay Open
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput…
View article: TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples
TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples Open
View article: Impact of Mitochondrial Fatty Acid Synthesis on Mitochondrial Biogenesis
Impact of Mitochondrial Fatty Acid Synthesis on Mitochondrial Biogenesis Open
View article: ACP Acylation Is an Acetyl-CoA-Dependent Modification Required for Electron Transport Chain Assembly
ACP Acylation Is an Acetyl-CoA-Dependent Modification Required for Electron Transport Chain Assembly Open
View article: Structure of the human Fe–S cluster assembly sub-complex: implications in Friedreich's ataxia and primary metabolism
Structure of the human Fe–S cluster assembly sub-complex: implications in Friedreich's ataxia and primary metabolism Open
Iron-sulfur (Fe-S) clusters are small, inorganic cofactors required for most living organisms. 1hese cofactors play roles in many essential processes including oxidative respiration and DNA replication and repair.In eukaryotes, Fe-S cluste…
View article: Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions
Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions Open
Significance Prokaryotic and eukaryotic organisms use analogous pathways to synthesize protein cofactors called iron–sulfur clusters. An unexplained difference between pathways is the functional requirements of the respective cysteine desu…