Jonathan M.G. Higgins
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View article: Pharmacological approaches to understanding protein kinase signaling networks
Pharmacological approaches to understanding protein kinase signaling networks Open
Protein kinases play vital roles in controlling cell behavior, and an array of kinase inhibitors are used successfully for treatment of disease. Typical drug development pipelines involve biological studies to validate a protein kinase tar…
View article: Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation
Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation Open
Histone modifications influence the recruitment of reader proteins to chromosomes to regulate events including transcription and cell division. The idea of a histone code, where combinations of modifications specify unique downstream funct…
View article: Acute brain slice elastic modulus decreases over time
Acute brain slice elastic modulus decreases over time Open
A common benchmark in the brain tissue mechanics literature is that the properties of acute brain slices should be measured within 8 h of the experimental animal being sacrificed. The core assumption is that—since there is no substantial p…
View article: Acute brain slice elastic modulus decreases over time
Acute brain slice elastic modulus decreases over time Open
A common benchmark in the brain tissue mechanics literature is that the properties of acute brain slices should be measured within 8 hours of the experimental animal being sacrificed. The core assumption is that — since there is no substan…
View article: Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation
Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation Open
Histone modifications influence the recruitment of reader proteins to chromosomes to regulate events including transcription and cell division. The idea of a histone code, where particular combinations of modifications specify unique downs…
View article: Robustness of NanoBiT luciferase complementation technology in the presence of widely used kinase inhibitors
Robustness of NanoBiT luciferase complementation technology in the presence of widely used kinase inhibitors Open
Bioluminescence assays using luciferase enzymes are widely used in research to monitor gene expression and an array of other cell properties, and split luciferase enzymes can be used to measure protein interactions in biochemical assays an…
View article: Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis
Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis Open
Protein kinases that phosphorylate histones are ideally-placed to influence the behavior of chromosomes during cell division. Indeed, a number of conserved histone phosphorylation events occur prominently during mitosis and meiosis in most…
View article: PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals
PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals Open
PCTAIRE1 (also known as CDK16) is a serine-threonine kinase implicated in physiological processes like neuronal development, vesicle trafficking, spermatogenesis and cell proliferation. However, its exact role in cell division remains uncl…
View article: The Aurora B gradient sustains kinetochore stability in anaphase
The Aurora B gradient sustains kinetochore stability in anaphase Open
Kinetochores assemble on chromosomes in mitosis to allow microtubules to attach and bring about accurate chromosome segregation. The kinases Cyclin B-Cdk1 and Aurora B are crucial for the formation of stable kinetochores. However, the acti…
View article: Dissecting the roles of Haspin and VRK1 in Histone H3 threonine-3 phosphorylation during mitosis
Dissecting the roles of Haspin and VRK1 in Histone H3 threonine-3 phosphorylation during mitosis Open
Protein kinases that phosphorylate histones are ideally-placed to influence the behavior of chromosomes during cell division. Indeed, a number of conserved histone phosphorylation events occur prominently during mitosis and meiosis in most…
View article: The Aurora B gradient sustains kinetochore stability in anaphase
The Aurora B gradient sustains kinetochore stability in anaphase Open
Kinetochores assemble on chromosomes in mitosis to allow microtubules to attach and bring about accurate chromosome segregation. The kinases Cyclin B-Cdk1 and Aurora B are crucial for the formation of stable kinetochores. However, the acti…
View article: Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability
Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability Open
Collisions between the DNA replication machinery and co-transcriptional R-loops can impede DNA synthesis and are a major source of genomic instability in cancer cells. How cancer cells deal with R-loops to proliferate is poorly understood.…
View article: Priming chromatin for segregation: functional roles of mitotic histone modifications
Priming chromatin for segregation: functional roles of mitotic histone modifications Open
Posttranslational modifications (PTMs) of histone proteins are important for various cellular processes including regulation of gene expression and chromatin structure, DNA damage response and chromosome segregation. Here we comprehensivel…
View article: CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation
CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation Open
Faithful mitotic chromosome segregation is required for the maintenance of genomic stability. We discovered the phosphorylation of histone H2B at serine 6 (H2B S6ph) as a new chromatin modification site and found that this modification occ…
View article: The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression Open
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1,…