Jonathan N. Pruneda
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View article: Hijacking the Ubl code: bacterial manipulation of ubiquitin-like proteins
Hijacking the Ubl code: bacterial manipulation of ubiquitin-like proteins Open
Ubiquitin (Ub) and Ub-like (Ubl) signaling processes regulate broad aspects of eukaryotic cellular biology. Conserved sets of enzymes control the covalent attachment of Ub/Ubl onto proteins, and disruption of these highly regulated process…
View article: Differential targeting of human pyroptotic Caspase-5 and Caspase-4 by <i>Shigella</i> OspC2 and OspC3
Differential targeting of human pyroptotic Caspase-5 and Caspase-4 by <i>Shigella</i> OspC2 and OspC3 Open
Pyroptosis is an inflammatory cell death pathway that is a key defense mechanism of intestinal epithelial cells. To successfully establish an infection, intracytosolic Gram-negative pathogens must block this host response. Indeed, Shigella…
View article: RNF114 and RNF166 exemplify reader-writer E3 ligases that extend K11 polyubiquitin onto sites of MARUbylation
RNF114 and RNF166 exemplify reader-writer E3 ligases that extend K11 polyubiquitin onto sites of MARUbylation Open
Ubiquitin (Ub) cooperates with other post-translational modifications to provide a tiered opportunity for protein regulation. Deltex E3 ligases were previously implicated in ubiquitylation of ADP-ribose (ADPr)-containing macromolecules in …
View article: Novel SUCLG1 Variants in a Patient with Fatal Neonatal Onset Succinyl-Coa Ligase Deficiency
Novel SUCLG1 Variants in a Patient with Fatal Neonatal Onset Succinyl-Coa Ligase Deficiency Open
View article: Proximity biotinylation at the host- <i>Shigella</i> interface reveals UFMylation as an antibacterial pathway
Proximity biotinylation at the host- <i>Shigella</i> interface reveals UFMylation as an antibacterial pathway Open
Host cells contest invasion by intracellular bacterial pathogens with multiple strategies that recognise and / or damage the bacterial surface. To identify novel host defence factors targeted to intracellular bacteria, we developed a versa…
View article: A family of E3 ligases extend K11 polyubiquitin on sites of MARUbylation
A family of E3 ligases extend K11 polyubiquitin on sites of MARUbylation Open
Ubiquitin (Ub) cooperation with other post-translational modifications provides a tiered opportunity for protein regulation. Small modifications to Ub such as phosphorylation, acetylation, or ADP-ribosylation have varying impacts on signal…
View article: Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose
Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose Open
View article: Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation
Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation Open
The immune checkpoint regulator CTLA4 is an unusually short-lived membrane protein. Here, we show that its lysosomal degradation is dependent on ubiquitylation at lysine residues 203 and 213. Inhibition of the v-ATPase partially restores C…
View article: A functional screen for ubiquitin regulation identifies an E3 ligase secreted by<i>Pseudomonas aeruginosa</i>
A functional screen for ubiquitin regulation identifies an E3 ligase secreted by<i>Pseudomonas aeruginosa</i> Open
Ubiquitin signaling controls many aspects of eukaryotic biology, including targeted protein degradation and immune defense. Remarkably, invading bacterial pathogens have adapted secreted effector proteins that hijack host ubiquitination to…
View article: Discovery of ester-linked ubiquitylation of PARP10 mono-ADP-ribosylation in cells: a dual post-translational modification on Glu/Asp side chains
Discovery of ester-linked ubiquitylation of PARP10 mono-ADP-ribosylation in cells: a dual post-translational modification on Glu/Asp side chains Open
The prevailing view on post-translational modifications (PTMs) is that amino acid side chains in proteins are modified with a single PTM at any given time. However, a growing body of work has demonstrated crosstalk between different PTMs, …
View article: Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity
Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity Open
Aspects of how Burkholderia escape the host's intrinsic immune response to replicate in the cell cytosol remain enigmatic. Here, we show that Burkholderia has evolved two mechanisms to block the activity of Ring finger protein 213 (RNF213)…
View article: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation
The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation Open
Many cellular processes are regulated by ubiquitin-mediated proteasomal degradation. Pathogens can regulate eukaryotic proteolysis through the delivery of proteins with de-ubiquitinating (DUB) activities. The obligate intracellular pathoge…
View article: Author Response: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation
Author Response: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation Open
View article: Author Response: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation
Author Response: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation Open
Many cellular processes are regulated by ubiquitin-mediated proteasomal degradation. Pathogens can regulate eukaryotic proteolysis through the delivery of proteins with de-ubiquitinating (DUB) activities. The obligate intracellular pathoge…
View article: The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation
The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation Open
Many cellular processes are regulated by ubiquitin-mediated proteasomal degradation. Pathogens can regulate eukaryotic proteolysis through the delivery of proteins with de-ubiquitinating (DUB) activities. The obligate intracellular pathoge…
View article: Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation
Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation Open
The immune checkpoint regulator CTLA4 is an unusually short-lived membrane protein. Here we show that its lysosomal degradation is dependent on ubiquitylation at Lysine residues 203 and 213. Inhibition of the v-ATPase partially restores CT…
View article: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and endoplasmic reticulum stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal re…
View article: Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Full text Figures and data Side by side Abstract eLife assessment eLife digest Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Metrics Abstract P…
View article: Bacterial ligases reveal fundamental principles of polyubiquitin specificity
Bacterial ligases reveal fundamental principles of polyubiquitin specificity Open
View article: Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Reviewer #1 (Public Review): Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Reviewer #1 (Public Review): Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Reviewer #2 (Public Review): Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Reviewer #2 (Public Review): Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Ubiquitin‐targeted bacterial effectors: rule breakers of the ubiquitin system
Ubiquitin‐targeted bacterial effectors: rule breakers of the ubiquitin system Open
View article: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and endoplasmic reticulum stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal re…
View article: Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays
Author Response: Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Allelic strengths of encephalopathy-associated <i>UBA5</i> variants correlate between <i>in vivo</i> and <i>in vitro</i> assays
Allelic strengths of encephalopathy-associated <i>UBA5</i> variants correlate between <i>in vivo</i> and <i>in vitro</i> assays Open
Protein UFMylation downstream of the E1 enzyme UBA5 plays essential roles in development and ER stress. Variants in the UBA5 gene are associated with developmental and epileptic encephalopathy 44 (DEE44), an autosomal recessive disorder ch…
View article: Dedicated bacterial esterases reverse lipopolysaccharide ubiquitylation to block immune sensing
Dedicated bacterial esterases reverse lipopolysaccharide ubiquitylation to block immune sensing Open
View article: Bacterial mimicry of eukaryotic HECT ubiquitin ligation
Bacterial mimicry of eukaryotic HECT ubiquitin ligation Open
HECT E3 ubiquitin (Ub) ligases direct their modified substrates toward a range of cellular fates dictated by the specific form of monomeric or polymeric Ub (polyUb) signal that is attached. How polyUb specificity is achieved has been a lon…