Jonathan S. Oakhill
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View article: Long-read sequencing-based atlas of tissue-specific expression of Drp1 transcript variants
Long-read sequencing-based atlas of tissue-specific expression of Drp1 transcript variants Open
Dynamin-related protein 1 (Drp1), encoded by DNM1L , is essential for mitochondrial fission, but its functional roles remain unclear due to isoform-specific effects from alternative splicing. Short-read RNA sequencing fails to resolve full…
View article: Lysine acetylation modulates s‐<scp>OPA1 GTPase</scp> activity and oligomerization in mitochondrial membrane remodeling
Lysine acetylation modulates s‐<span>OPA1 GTPase</span> activity and oligomerization in mitochondrial membrane remodeling Open
Mitochondrial dynamics are regulated by coordinated fission and fusion events that rely on key proteins and lipids organized spatially within the mitochondria. The dynamin‐related GTPase Optic Atrophy 1 (OPA1) is essential for inner mitoch…
View article: AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates
AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates Open
View article: Reversal of metformin’s anti-proliferative effect in fission yeast efr3 and dnm1 (DRP1) mutants with elongated mitochondria
Reversal of metformin’s anti-proliferative effect in fission yeast efr3 and dnm1 (DRP1) mutants with elongated mitochondria Open
Metformin is a well-tolerated drug frequently prescribed for managing type 2 diabetes. Extended metformin use has been linked to a significant decrease in cancer incidence across both diabetic and non-diabetic populations. Here we investig…
View article: Salicylate-Elicited Activation of AMP-Activated Protein Kinase Directly Triggers Degradation of C-Myc in Colorectal Cancer Cells
Salicylate-Elicited Activation of AMP-Activated Protein Kinase Directly Triggers Degradation of C-Myc in Colorectal Cancer Cells Open
Aspirin has consistently shown preventive effects in some solid cancers, notably colorectal cancer. However, the precise molecular mechanisms underlying this positive effect have remained elusive. In this study, we used an azoxymethane-ind…
View article: The role of STK11/LKB1 in cancer biology: implications for ovarian tumorigenesis and progression
The role of STK11/LKB1 in cancer biology: implications for ovarian tumorigenesis and progression Open
STK11 (serine-threonine kinase 11), also known as LKB1 (liver kinase B1) is a highly conserved master kinase that regulates cellular metabolism and polarity through a complex signaling network involving AMPK and 12 other AMPK-related kinas…
View article: Convergence on CaMK4: a key modulator of autism-associated signaling pathways in neurons
Convergence on CaMK4: a key modulator of autism-associated signaling pathways in neurons Open
View article: Convergence on CaMK4: A Key Modulator of Autism-Associated Signaling Pathways in Neurons
Convergence on CaMK4: A Key Modulator of Autism-Associated Signaling Pathways in Neurons Open
Although the precise underlying cause(s) of autism spectrum disorder remain unclear, more than 1000 rare genetic variations are associated with the condition. For many people living with profound autism, this genetic heterogeneity has impe…
View article: The metabolic sensor AMPK: Twelve enzymes in one
The metabolic sensor AMPK: Twelve enzymes in one Open
View article: New developments in AMPK and mTORC1 cross-talk
New developments in AMPK and mTORC1 cross-talk Open
Metabolic homeostasis and the ability to link energy supply to demand are essential requirements for all living cells to grow and proliferate. Key to metabolic homeostasis in all eukaryotes are AMPK and mTORC1, two kinases that sense nutri…
View article: The AMPK activator ATX-304 alters cellular metabolism to protect against cisplatin-induced acute kidney injury
The AMPK activator ATX-304 alters cellular metabolism to protect against cisplatin-induced acute kidney injury Open
Acute kidney injury (AKI) disrupts energy metabolism. Targeting metabolism through AMP-activated protein kinase (AMPK) may alleviate AKI. ATX-304, a pan-AMPK activator, was evaluated in C57Bl/6 mice and tubular epithelial cell (TEC) cultur…
View article: Modern Insights into Muscle Glycogen Phosphorylase Activity
Modern Insights into Muscle Glycogen Phosphorylase Activity Open
Recent identification of new human muscle glycogen phosphorylation sites has renewed interest in understanding human variations in the regulation of glycogen metabolism and glucose homeostasis. This paper presents a detailed method for the…
View article: AMPK protects endothelial cells against HSV-1 replication via inhibition of mTORC1 and ACC1
AMPK protects endothelial cells against HSV-1 replication via inhibition of mTORC1 and ACC1 Open
Herpes simplex virus type 1 (HSV-1) is a widespread contagious pathogen, mostly causing mild symptoms on the mucosal entry side. However, systemic distribution, in particular upon reactivation of the virus in immunocompromised patients, ma…
View article: CaMKK2 is not involved in contraction-stimulated AMPK activation and glucose uptake in skeletal muscle
CaMKK2 is not involved in contraction-stimulated AMPK activation and glucose uptake in skeletal muscle Open
We demonstrate that pharmacological inhibition or genetic loss of CaMKK2 does not affect contraction-stimulated AMPK phosphorylation and activation, as well as glucose uptake in skeletal muscle. Previously observed inhibitory effect of STO…
View article: Elevated basal AMP-activated protein kinase activity sensitizes colorectal cancer cells to growth inhibition by metformin
Elevated basal AMP-activated protein kinase activity sensitizes colorectal cancer cells to growth inhibition by metformin Open
Expression and activity of the AMP-activated protein kinase (AMPK) α 1 catalytic subunit of the heterotrimeric kinase significantly correlates with poor outcome for colorectal cancer patients. Hence there is considerable interest in uncove…
View article: Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women Open
Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
View article: Data from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
Data from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women Open
Epidemiologic evidence supports a correlation between obesity and breast cancer in women. AMP-activated protein kinase plays an important role in energy homeostasis and inhibits the actions of cyclic AMP-responsive element binding protein-…
View article: Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women Open
Supplementary Figure 1 from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
View article: Data from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women
Data from Subcellular Localization of Cyclic AMP-Responsive Element Binding Protein-Regulated Transcription Coactivator 2 Provides a Link between Obesity and Breast Cancer in Postmenopausal Women Open
Epidemiologic evidence supports a correlation between obesity and breast cancer in women. AMP-activated protein kinase plays an important role in energy homeostasis and inhibits the actions of cyclic AMP-responsive element binding protein-…
View article: SGC-CAMKK2-1: A Chemical Probe for CAMKK2
SGC-CAMKK2-1: A Chemical Probe for CAMKK2 Open
The serine/threonine protein kinase calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) plays critical roles in a range of biological processes. Despite its importance, only a handful of inhibitors of CAMKK2 have been disclosed. …
View article: SGC-CAMKK2-1: A Chemical Probe for CAMKK2
SGC-CAMKK2-1: A Chemical Probe for CAMKK2 Open
The serine/threonine protein kinase calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) plays critical roles in a range of biological processes. Despite its importance, only a handful of inhibitors of CAMKK2 have been disclosed. …
View article: A novel small molecule inhibitor of human Drp1
A novel small molecule inhibitor of human Drp1 Open
View article: Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis
Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis Open
AMP-activated protein kinase (AMPK) is a master regulator of cellular energy homeostasis and a therapeutic target for metabolic diseases. Co/post-translational N-myristoylation of glycine-2 (Gly2) of the AMPK β subunit has been suggested t…
View article: SGC-CAMKK2-1: A chemical probe for CAMKK2
SGC-CAMKK2-1: A chemical probe for CAMKK2 Open
The serine/threonine protein kinase calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) plays critical roles in a range of biological processes. Despite its importance, only a handful of inhibitors of CAMKK2 have been disclosed. …
View article: Genetic impairment of succinate metabolism disrupts bioenergetic sensing in adrenal neuroendocrine cancer
Genetic impairment of succinate metabolism disrupts bioenergetic sensing in adrenal neuroendocrine cancer Open
View article: Systemic Ablation of Camkk2 Impairs Metastatic Colonization and Improves Insulin Sensitivity in TRAMP Mice: Evidence for Cancer Cell-Extrinsic CAMKK2 Functions in Prostate Cancer
Systemic Ablation of Camkk2 Impairs Metastatic Colonization and Improves Insulin Sensitivity in TRAMP Mice: Evidence for Cancer Cell-Extrinsic CAMKK2 Functions in Prostate Cancer Open
Despite early studies linking calcium-calmodulin protein kinase kinase 2 (CAMKK2) to prostate cancer cell migration and invasion, the role of CAMKK2 in metastasis in vivo remains unclear. Moreover, while CAMKK2 is known to regulate systemi…
View article: Structure-function analysis of the AMPK activator SC4 and identification of a potent pan AMPK activator
Structure-function analysis of the AMPK activator SC4 and identification of a potent pan AMPK activator Open
The AMP-activated protein kinase (AMPK) αβγ heterotrimer is a primary cellular energy sensor and central regulator of energy homeostasis. Activating skeletal muscle AMPK with small molecule drugs improves glucose uptake and provides an opp…
View article: Systemic ablation of <i>Camkk2</i> impairs metastatic colonization and improves insulin sensitivity in TRAMP mice: Evidence for cancer cell-extrinsic CAMKK2 functions in prostate cancer
Systemic ablation of <i>Camkk2</i> impairs metastatic colonization and improves insulin sensitivity in TRAMP mice: Evidence for cancer cell-extrinsic CAMKK2 functions in prostate cancer Open
Despite early studies linking calcium-calmodulin protein kinase kinase 2 (CAMKK2) to prostate cancer cell migration and invasion, the role of CAMKK2 in metastasis in vivo remains unclear. Moreover, while CAMKK2 is known to regulate systemi…
View article: An AMPKα2-specific phospho-switch controls lysosomal targeting for activation
An AMPKα2-specific phospho-switch controls lysosomal targeting for activation Open
AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are metabolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S3…
View article: Genetic Impairment of Succinate Metabolism Disrupts Bioenergetic Sensing in Adrenal Neuroendocrine Cancer
Genetic Impairment of Succinate Metabolism Disrupts Bioenergetic Sensing in Adrenal Neuroendocrine Cancer Open
SUMMARY Metabolic dysfunction mutations can impair energy sensing and cause cancer. Loss of function of mitochondrial TCA cycle enzyme, succinate dehydrogenase B (SDHB) results in various forms of cancer typified by pheochromocytoma (PC). …