Jonathan T. Sullivan
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View article: The emergence of cell-based protein arrays to test for polyspecific off-target binding of antibody therapeutics
The emergence of cell-based protein arrays to test for polyspecific off-target binding of antibody therapeutics Open
Specificity profiling is a requirement for monoclonal antibodies (mAbs) and antibody-directed biotherapeutics such as CAR-T cells prior to initiating human trials. However, traditional approaches to assess the specificity of mAbs, primaril…
View article: Characterization of NLRP12 during the In Vivo Host Immune Response to Klebsiella pneumoniae and Mycobacterium tuberculosis
Characterization of NLRP12 during the In Vivo Host Immune Response to Klebsiella pneumoniae and Mycobacterium tuberculosis Open
The majority of nucleotide binding domain leucine rich repeats-containing (NLR) family members has yet to be functionally characterized. Of the described NLRs, most are considered to be proinflammatory and facilitate IL-1β production. Howe…
View article: Granuloma Formation and Host Defense in Chronic Mycobacterium tuberculosis Infection Requires PYCARD/ASC but Not NLRP3 or Caspase-1
Granuloma Formation and Host Defense in Chronic Mycobacterium tuberculosis Infection Requires PYCARD/ASC but Not NLRP3 or Caspase-1 Open
The NLR gene family mediates host immunity to various acute pathogenic stimuli, but its role in chronic infection is not known. This paper addressed the role of NLRP3 (NALP3), its adaptor protein PYCARD (ASC), and caspase-1 during infectio…
View article: Protein export systems of Mycobacterium tuberculosis : novel targets for drug development?
Protein export systems of Mycobacterium tuberculosis : novel targets for drug development? Open
Protein export is essential in all bacteria and many bacterial pathogens depend on specialized protein export systems for virulence. In Mycobacterium tuberculosis, the etiological agent of the disease tuberculosis, the conserved general se…
View article: Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage
Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage Open
The Myo/Nog cell lineage was discovered in the chick embryo and is also present in adult mammalian tissues. The cells are named for their expression of mRNA for the skeletal muscle specific transcription factor MyoD and bone morphogenetic …
View article: An orphaned Mce-associated membrane protein of M ycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters: OmamA functions in virulence and Mce stability
An orphaned Mce-associated membrane protein of M ycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters: OmamA functions in virulence and Mce stability Open
Mycobacterium tuberculosis proteins that are exported out of the bacterial cytoplasm are ideally positioned to be virulence factors; however, the functions of individual exported proteins remain largely unknown. Previous studies identified…
View article: The role of the Mycobacterium tuberculosis SecA2 protein export pathway in virulence
The role of the Mycobacterium tuberculosis SecA2 protein export pathway in virulence Open
Mycobacterium tuberculosis is an intracellular bacterial pathogen that replicates in macrophages in the lung of the host. The ability to replicate in these cells of the immune system is critical to the virulence of this important pathogen.…
View article: Monoclonal Antibody 2C6 Targets a Cross-Clade Conformational Epitope in gp41 with Highly Active Antibody-Dependent Cell Cytotoxicity
Monoclonal Antibody 2C6 Targets a Cross-Clade Conformational Epitope in gp41 with Highly Active Antibody-Dependent Cell Cytotoxicity Open
This paper further defines the function and area of the HIV trimeric envelope protein targeted by the monoclonal antibody 2C6. 2C6 binding is influenced by amino acid mutations across two separate gp41 sections of the envelope trimer. This…
Increased breadth of HIV-1 neutralization achieved by diverse antibody clones each with limited neutralization breadth Open
Broadly neutralizing antibodies (bNAbs) are rarely elicited by current human immunodeficiency virus type 1 (HIV-1) vaccine designs, but the presence of bNAbs in naturally infected individuals may be associated with high plasma viral loads,…
View article: Isolation of state-dependent monoclonal antibodies against the 12-transmembrane domain glucose transporter 4 using virus-like particles
Isolation of state-dependent monoclonal antibodies against the 12-transmembrane domain glucose transporter 4 using virus-like particles Open
Significance Generating mAbs against the native extracellular epitopes of multispanning membrane proteins is challenging, and as a result, few nonpeptidic mAbs against transporters have ever been isolated. Our approach here using virus-lik…
View article: The SecA2 pathway of Mycobacterium tuberculosis exports effectors that work in concert to arrest phagosome and autophagosome maturation
The SecA2 pathway of Mycobacterium tuberculosis exports effectors that work in concert to arrest phagosome and autophagosome maturation Open
To subvert host defenses, Mycobacterium tuberculosis (Mtb) avoids being delivered to degradative phagolysosomes in macrophages by arresting the normal host process of phagosome maturation. Phagosome maturation arrest by Mtb involves multip…
View article: High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers
High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers Open
Soluble envelope glycoprotein (Env) trimers (SOSIP.664 gp140) are attractive HIV-1 vaccine candidates, with structures that mimic the native membrane-bound Env spike (gp160). Since engineering trimers can be limited by the difficulty of ra…
View article: An orphaned <scp>M</scp>ce‐associated membrane protein of <scp><i>M</i></scp><i>ycobacterium tuberculosis</i> is a virulence factor that stabilizes <scp>M</scp>ce transporters
An orphaned <span>M</span>ce‐associated membrane protein of <span><i>M</i></span><i>ycobacterium tuberculosis</i> is a virulence factor that stabilizes <span>M</span>ce transporters Open
Summary Mycobacterium tuberculosis proteins that are exported out of the bacterial cytoplasm are ideally positioned to be virulence factors; however, the functions of individual exported proteins remain largely unknown. Previous studies id…