Jonathan Walklate
YOU?
Author Swipe
View article: Distinct effects of two hearing loss–associated mutations in the sarcomeric myosin MYH7b
Distinct effects of two hearing loss–associated mutations in the sarcomeric myosin MYH7b Open
For decades, sarcomeric myosin heavy chain proteins were assumed to be restricted to striated muscle where they function as molecular motors that contract muscle. However, MYH7b, an evolutionarily ancient member of this myosin family, has …
View article: Pseudo-phosphorylation of essential light chains affects the functioning of skeletal muscle myosin
Pseudo-phosphorylation of essential light chains affects the functioning of skeletal muscle myosin Open
View article: Single-molecule imaging reveals how mavacamten and PKA modulate ATP turnover in skeletal muscle myofibrils
Single-molecule imaging reveals how mavacamten and PKA modulate ATP turnover in skeletal muscle myofibrils Open
Muscle contraction is controlled at two levels: the thin and the thick filaments. The latter level of control involves three states of myosin heads: active, disordered relaxed (DRX), and super-relaxed (SRX), the distribution of which contr…
View article: Functional divergence of the sarcomeric myosin, MYH7b, supports species-specific biological roles
Functional divergence of the sarcomeric myosin, MYH7b, supports species-specific biological roles Open
View article: Exploring the super-relaxed state of myosin in myofibrils from fast-twitch, slow-twitch, and cardiac muscle
Exploring the super-relaxed state of myosin in myofibrils from fast-twitch, slow-twitch, and cardiac muscle Open
Muscle myosin heads, in the absence of actin, have been shown to exist in two states, the relaxed (turnover ∼0.05 s-1) and super-relaxed states (SRX, 0.005 s-1) using a simple fluorescent ATP chase assay (Hooijman, P.…
View article: Alpha and beta myosin isoforms and human atrial and ventricular contraction
Alpha and beta myosin isoforms and human atrial and ventricular contraction Open
View article: Identification of sequence changes in myosin II that adjust muscle contraction velocity
Identification of sequence changes in myosin II that adjust muscle contraction velocity Open
The speed of muscle contraction is related to body size; muscles in larger species contract at slower rates. Since contraction speed is a property of the myosin isoform expressed in a muscle, we investigated how sequence changes in a range…
View article: Cryo-EM and Molecular Docking Shows Myosin Loop 4 Contacts Actin and Tropomyosin on Thin Filaments
Cryo-EM and Molecular Docking Shows Myosin Loop 4 Contacts Actin and Tropomyosin on Thin Filaments Open
View article: Myosin motor domains carrying mutations implicated in early or late onset hypertrophic cardiomyopathy have similar properties
Myosin motor domains carrying mutations implicated in early or late onset hypertrophic cardiomyopathy have similar properties Open
Hypertrophic cardiomyopathy (HCM) is a common genetic disorder characterized by left ventricular hypertrophy and cardiac hyper-contractility. Mutations in the β-cardiac myosin heavy chain gene (β-MyHC) are a major cause of HCM, but …
View article: The ATPase cycle of human muscle myosin II isoforms: Adaptation of a single mechanochemical cycle for different physiological roles
The ATPase cycle of human muscle myosin II isoforms: Adaptation of a single mechanochemical cycle for different physiological roles Open
View article: The ATPase cycle of Human Muscle Myosin II Isoforms: Adaptation of a single mechanochemical cycle for different physiological roles
The ATPase cycle of Human Muscle Myosin II Isoforms: Adaptation of a single mechanochemical cycle for different physiological roles Open
Striated muscle myosins are encoded by a large gene family in all mammals, including human. These isoforms define several of the key characteristics of the different striated muscle fiber types including maximum shortening velocity. We hav…
View article: Myosin motor domains carrying mutations implicated in early or late onset Hypertrophic Cardiomyopathy have similar properties
Myosin motor domains carrying mutations implicated in early or late onset Hypertrophic Cardiomyopathy have similar properties Open
Hypertrophic Cardiomyopathy (HCM) is a common genetic disorder that typically involves left ventricular hypertrophy and cardiac hypercontractility. Mutations in β cardiac myosin heavy chain ( β-MyHC ) are a major cause of HCM, but the spec…
View article: Modeling the Actin.myosin ATPase Cross-Bridge Cycle for Skeletal and Cardiac Muscle Myosin Isoforms
Modeling the Actin.myosin ATPase Cross-Bridge Cycle for Skeletal and Cardiac Muscle Myosin Isoforms Open
View article: Modeling the ATPase Cycle of Human Myosin II Isoforms and their Mutations
Modeling the ATPase Cycle of Human Myosin II Isoforms and their Mutations Open
View article: Kinetic Characterisation of Disease Causing Mutations in the Embryonic and ß-Cardiac Myosin Motor Domain
Kinetic Characterisation of Disease Causing Mutations in the Embryonic and ß-Cardiac Myosin Motor Domain Open
Myosin myopathies are a growing area of research not only to understand the nature of the disease and how it can occur, but also to gain insight into how the myosin molecule works. Point mutations are a great way of examining how regions o…
View article: Adaptation of mammalian myosin II sequences to body mass
Adaptation of mammalian myosin II sequences to body mass Open
The speed of muscle contraction is related to body size; muscles in larger species contract at a slower rate. We investigated the evolution of twelve myosin II isoforms to identify any adapted to increasing body mass in mammals. We identif…
View article: The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects
The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects Open
The embryonic myosin isoform is expressed during fetal development and rapidly down-regulated after birth. Freeman-Sheldon syndrome (FSS) is a disease associated with missense mutations in the motor domain of this myosin. It is the most se…
View article: β-MYHC Mutations Linked to Early-Onset HCM and DCM Show Differences in Pre-Steady and Steady State Kinetic Parameters
β-MYHC Mutations Linked to Early-Onset HCM and DCM Show Differences in Pre-Steady and Steady State Kinetic Parameters Open
View article: Myosin isoforms and the mechanochemical cross-bridge cycle
Myosin isoforms and the mechanochemical cross-bridge cycle Open
At the latest count the myosin family includes 35 distinct groups, all of which have the conserved myosin motor domain attached to a neck or lever arm, followed by a highly variable tail or cargo binding region. The motor domain has an ATP…
View article: Functional Analysis of Freeman-Sheldon Syndrome Causing Mutations on Embryonic Myosin
Functional Analysis of Freeman-Sheldon Syndrome Causing Mutations on Embryonic Myosin Open