Jonathon A. Ditlev
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View article: Biomolecular phase boundaries are described by a solubility product that accounts for variable stoichiometry and soluble oligomers
Biomolecular phase boundaries are described by a solubility product that accounts for variable stoichiometry and soluble oligomers Open
The solubility product is a rigorous description of the phase boundary for salt precipitation and has previously been shown to qualitatively describe the condensation of biomolecules. Here we present a derivation of the solubility product …
View article: Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes
Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes Open
The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel whose dysfunction leads to intracellular accumulation of chloride ions, dehydration of cell surfaces, and subsequent damage to airway and ductal organs. B…
View article: Disease-linked mutations dysregulate neuronal condensate physical properties, composition, and RNA translation
Disease-linked mutations dysregulate neuronal condensate physical properties, composition, and RNA translation Open
Local RNA translation is essential for development. In neurons, deficient local translation linked with mutations in scaffold proteins results in dysregulated dendrite and dendritic spine growth. However, mechanisms by which these proteins…
View article: Measurement of solubility product reveals the interplay of oligomerization and self-association for defining condensate formation
Measurement of solubility product reveals the interplay of oligomerization and self-association for defining condensate formation Open
Cellular condensates often consist of 10s to 100s of distinct interacting molecular species. Because of the complexity of these interactions, predicting the point at which they will undergo phase separation is daunting. Using experiments a…
View article: Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes
Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes Open
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride channel whose dysfunction leads to intracellular accumulation of chloride ions, dehydration of cell surfaces, and subsequent damage to airway and ductal organs. B…
View article: Measurement of solubility product in a model condensate reveals the interplay of small oligomerization and self-association
Measurement of solubility product in a model condensate reveals the interplay of small oligomerization and self-association Open
Cellular condensates often consist of 10s to 100s of distinct interacting molecular species. Because of the complexity of these interactions, predicting the point at which they will undergo phase separation into discrete compartments is da…
View article: Coupling of protein condensates to ordered lipid domains determines functional membrane organization
Coupling of protein condensates to ordered lipid domains determines functional membrane organization Open
During T cell activation, the transmembrane adaptor protein LAT (linker for activation of T cells) forms biomolecular condensates with Grb2 and Sos1, facilitating signaling. LAT has also been associated with cholesterol-rich condensed lipi…
View article: Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma
Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma
View article: Data from A Molecular Classification of Papillary Renal Cell Carcinoma
Data from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Despite the moderate incidence of papillary renal cell carcinoma (PRCC), there is a disproportionately limited understanding of its underlying genetic programs. There is no effective therapy for metastatic PRCC, and patients are often excl…
View article: Data from A Molecular Classification of Papillary Renal Cell Carcinoma
Data from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Despite the moderate incidence of papillary renal cell carcinoma (PRCC), there is a disproportionately limited understanding of its underlying genetic programs. There is no effective therapy for metastatic PRCC, and patients are often excl…
View article: Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma
Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Supplementary Figures C1-C2 from A Molecular Classification of Papillary Renal Cell Carcinoma
View article: Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma
Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma
View article: Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma
Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma Open
Supplementary Table 1 from A Molecular Classification of Papillary Renal Cell Carcinoma
View article: Coupling of protein condensates to ordered lipid domains determines functional membrane organization
Coupling of protein condensates to ordered lipid domains determines functional membrane organization Open
During T-cell activation, the transmembrane adaptor Linker of Activation of T-cells (LAT) forms biomolecular condensates with Grb2 and Sos1, facilitating signaling. LAT has also been associated with cholesterol-rich condensed lipid domains…
View article: What are the distinguishing features and size requirements of biomolecular condensates and their implications for RNA-containing condensates?
What are the distinguishing features and size requirements of biomolecular condensates and their implications for RNA-containing condensates? Open
Exciting recent work has highlighted that numerous cellular compartments lack encapsulating lipid bilayers (often called “membraneless organelles”), and that their structure and function are central to the regulation of key biological proc…
View article: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains
The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains Open
Sigma 1 receptor (S1R) is a 223-amino-acid-long transmembrane endoplasmic reticulum (ER) protein. S1R modulates activity of multiple effector proteins and is a well-established drug target. However, signaling functions of S1R in cells are …
View article: The role of sigma-1 receptor in organization of endoplasmic reticulum signaling microdomains
The role of sigma-1 receptor in organization of endoplasmic reticulum signaling microdomains Open
SUMMARY Sigma 1 receptor (S1R) is a 223 amino acid-long transmembrane endoplasmic reticulum (ER) protein. S1R modulates activity of multiple effector proteins but its signaling functions are poorly understood. We here test the hypothesis t…
View article: A composition-dependent molecular clutch between T cell signaling condensates and actin
A composition-dependent molecular clutch between T cell signaling condensates and actin Open
During T cell activation, biomolecular condensates form at the immunological synapse (IS) through multivalency-driven phase separation of LAT, Grb2, Sos1, SLP-76, Nck, and WASP. These condensates move radially at the IS, traversing success…
View article: Regulation of Transmembrane Signaling by Phase Separation
Regulation of Transmembrane Signaling by Phase Separation Open
Cell surface transmembrane receptors often form nanometer- to micrometer-scale clusters to initiate signal transduction in response to environmental cues. Extracellular ligand oligomerization, domain-domain interactions, and binding to mul…
View article: The mechanisms of dynamin-actin interaction
The mechanisms of dynamin-actin interaction Open
Cell-cell fusion is an indispensable process in the conception, development and physiology of multicellular organisms. Here we demonstrate a direct and noncanonical role for dynamin, best known as a fission GTPase in endocytosis, in cell-c…
View article: A Composition-Dependent Molecular Clutch Between T Cell Signaling Condensates and Actin
A Composition-Dependent Molecular Clutch Between T Cell Signaling Condensates and Actin Open
Biomolecular condensates play important roles in eukaryotic cells by concentrating molecules into foci without a surrounding membrane. During T cell activation, biomolecular condensates form at the immunological synapse (IS) through multiv…