Joose Kreutzer
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View article: P02.29.B HYPOXIA SHAPES TUMOR IMMUNE MICROENVIRONMENT THROUGH CELL-TYPE DEPENDENT RESPONSES IN DIFFUSE ASTROCYTOMAS
P02.29.B HYPOXIA SHAPES TUMOR IMMUNE MICROENVIRONMENT THROUGH CELL-TYPE DEPENDENT RESPONSES IN DIFFUSE ASTROCYTOMAS Open
BACKGROUND Hypoxia is a critical driver of tumor aggressiveness in high-grade gliomas, yet its cell-type-specific effects on immune cell populations within the tumor microenvironment (TME) remain poorly understood. MATERIAL AND METHODS We …
View article: A hypoxia-on-a-chip platform for modeling ischemic arrhythmogenesis and evaluating the effects of levosimendan and OR-1896 on ischemic human iPSC-derived cardiomyocytes
A hypoxia-on-a-chip platform for modeling ischemic arrhythmogenesis and evaluating the effects of levosimendan and OR-1896 on ischemic human iPSC-derived cardiomyocytes Open
Acute hypoxia is a major contributor to cardiomyocyte damage and dysfunction in ischemic heart disease, and the effective therapeutic strategies remain limited. Levosimendan, a calcium sensitizer with both inotropic and vasodilatory effect…
View article: Patient-derived Lamin A/C mutant cardiomyocytes demonstrate altered electrophysiological characteristics and responses to hypoxia induced stress
Patient-derived Lamin A/C mutant cardiomyocytes demonstrate altered electrophysiological characteristics and responses to hypoxia induced stress Open
View article: Microelectrode array scaled for human hippocampal slices
Microelectrode array scaled for human hippocampal slices Open
Temporal lobe epilepsy (TLE) is a prevalent neurological disorder characterized by recurrent seizures originating from the cortex, amygdala and especially hippocampus. While two-thirds of TLE patients achieve seizure control through medica…
View article: Design, fabrication, and characterization of a user-friendly microfluidic device for studying liver zonation-on-chip (ZoC)
Design, fabrication, and characterization of a user-friendly microfluidic device for studying liver zonation-on-chip (ZoC) Open
View article: Barrier-free, open-top microfluidic chip for generating two distinct, interconnected 3D microvascular networks
Barrier-free, open-top microfluidic chip for generating two distinct, interconnected 3D microvascular networks Open
Developing microphysiological cell culture platforms with a three-dimensional (3D) microenvironment has been a significant advancement from traditional monolayer cultures. Still, most of the current microphysiological platforms are limited…
View article: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson’s Disease
Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson’s Disease Open
View article: Design, Fabrication, and Characterization of a User-Friendly Microfluidic Device for Studying Liver Zonation-on-Chip (ZoC)
Design, Fabrication, and Characterization of a User-Friendly Microfluidic Device for Studying Liver Zonation-on-Chip (ZoC) Open
Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which is challenging to recapitulate in traditional cell cultures. This study presents a novel microfluidic device designed to induce zonation in liver cel…
View article: Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson’s Disease
Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson’s Disease Open
Previously, several in vitro and in vivo studies have shown that the pathological hallmark of Parkinson’s disease (PD), malicious strains of alpha-synuclein (α-s) protein, are transferred between cells via different routes, thus participat…
View article: Oxygen gradient generator to improve in vitro modeling of ischemic stroke
Oxygen gradient generator to improve in vitro modeling of ischemic stroke Open
Introduction In the core of a brain infarct, perfusion is severely impeded, and neuronal death occurs within minutes. In the penumbra, an area near the core with more remaining perfusion, cells initially remain viable, but activity is sign…
View article: Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation
Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation Open
Ischemic heart disease is the most common cardiovascular disease and a major burden for healthcare worldwide. However, its pathophysiology is still not fully understood, and human-based models for disease mechanisms and treatments are need…
View article: Compartmentalized organ-on-a-chip structure for spatiotemporal control of oxygen microenvironments
Compartmentalized organ-on-a-chip structure for spatiotemporal control of oxygen microenvironments Open
View article: Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology
Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology Open
It is well established that mechanical cues, e.g., tensile- compressive- or shear forces, are important co-regulators of cell and tissue physiology. To understand the mechanistic effects these cues have on cells, technologies allowing prec…
View article: A human cell-based in vitro model of bone for defining the dormant niche components for disseminated breast and prostate cancer cells
A human cell-based in vitro model of bone for defining the dormant niche components for disseminated breast and prostate cancer cells Open
View article: Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Open
Ischemic heart disease (IHD) is one of the leading causes of mortality worldwide. Preserving functionality and preventing arrhythmias of the heart are key principles in the management of patients with IHD. Levosimendan, a unique calcium (C…
View article: M1‐linked ubiquitination facilitates NF‐κB activation and survival during sterile inflammation
M1‐linked ubiquitination facilitates NF‐κB activation and survival during sterile inflammation Open
Methionine 1 (M1)‐linked ubiquitination plays a key role in the regulation of inflammatory nuclear factor‐κB (NF‐κB) signalling and is important for clearance of pathogen infection in Drosophila melanogaster . M1‐linked ubiquitin (M1‐Ub) c…
View article: M1-linked ubiquitination facilitates NF-κB activation during sterile inflammation
M1-linked ubiquitination facilitates NF-κB activation during sterile inflammation Open
Methionine 1 (M1)-linked ubiquitination plays a key role in the regulation of inflammatory nuclear factor-κB (NF-κB) signalling and is important for clearance of pathogen infection in Drosophila melanogaster . M1-linked ubiquitin (M1-Ub) c…
View article: Human induced pluripotent stem cell-based platform for modeling cardiac ischemia
Human induced pluripotent stem cell-based platform for modeling cardiac ischemia Open
Ischemic heart disease is a major cause of death worldwide, and the only available therapy to salvage the tissue is reperfusion, which can initially cause further damage. Many therapeutics that have been promising in animal models have fai…
View article: Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity
Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity Open
View article: Cardiomyocytes: Analysis of Temperature Response and Signal Propagation Between Dissociated Clusters Using Novel Video-Based Movement Analysis Software
Cardiomyocytes: Analysis of Temperature Response and Signal Propagation Between Dissociated Clusters Using Novel Video-Based Movement Analysis Software Open
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide a great resource for functional cell and tissue models that can be applied in heart research, pharmaceutical industry, and future regenerative medicine. During …
View article: Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system
Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system Open
View article: Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes Open
In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial s…
View article: Modeling of LMNA-Related Dilated Cardiomyopathy Using Human Induced Pluripotent Stem Cells
Modeling of LMNA-Related Dilated Cardiomyopathy Using Human Induced Pluripotent Stem Cells Open
Dilated cardiomyopathy (DCM) is one of the leading causes of heart failure and heart transplantation. A portion of familial DCM is due to mutations in the LMNA gene encoding the nuclear lamina proteins lamin A and C and without adequate tr…
View article: Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro
Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro Open
View article: Microelectrode array for noninvasive analysis of cardiomyocytes at the single-cell level
Microelectrode array for noninvasive analysis of cardiomyocytes at the single-cell level Open
Microelectrode arrays (MEAs) are widely used to assess the electrophysiology of human pluripotent stem cell-derived cardiomyocytes (hPS-CMs). Traditionally, MEAs have been used to record data at the cell population level, but it would be b…
View article: A Portable Microscale Cell Culture System with Indirect Temperature Control
A Portable Microscale Cell Culture System with Indirect Temperature Control Open
A physiologically relevant environment is essential for successful long-term cell culturing in vitro. Precise control of temperature, one of the most crucial environmental parameters in cell cultures, increases the fidelity and repeatabili…
View article: P527Modeling DCM due to Lamin A/C gene mutation using hiPSC-CM
P527Modeling DCM due to Lamin A/C gene mutation using hiPSC-CM Open
View article: A Portable Live-Cell Imaging System With an Invert-Upright-Convertible Architecture and a Mini-Bioreactor for Long-Term Simultaneous Cell Imaging, Chemical Sensing, and Electrophysiological Recording
A Portable Live-Cell Imaging System With an Invert-Upright-Convertible Architecture and a Mini-Bioreactor for Long-Term Simultaneous Cell Imaging, Chemical Sensing, and Electrophysiological Recording Open
Cell culture in-vitro is a well-known method to develop cell and disease models for studying physiologically relevant mechanisms and responses for various applications in life sciences. Conventional methods for instance, using static cultu…
View article: Mini-incubator for prolonged hypoxia studies on MEA: Effect of hypoxia for IPSC-derived cardiomyocytes
Mini-incubator for prolonged hypoxia studies on MEA: Effect of hypoxia for IPSC-derived cardiomyocytes Open
Event Abstract Back to Event Mini-incubator for prolonged hypoxia studies on MEA: Effect of hypoxia for IPSC-derived cardiomyocytes Joose Kreutzer1*, Disheet Shah2, Kaisa Tornberg1, Antti-Juhana Mäki1, Mari Pekkanen-Mattila2, Katriina Aalt…
View article: A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture
A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture Open
Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coa…