J. Benz
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View article: Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation
Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation Open
T helper (Th) cell lineages are linked to metabolism, but precise mechanisms in human Th1 cells remain unclear. We addressed this question by in vitro stimulation and CRISPR/Cas9-mediated gene editing. Metabolic profiling revealed enhanced…
View article: A high-resolution data set of fatty acid-binding protein structures. II. Crystallographic overview, ligand classes and binding pose
A high-resolution data set of fatty acid-binding protein structures. II. Crystallographic overview, ligand classes and binding pose Open
Fatty acid-binding protein isoforms 4 and 5 are potential diabetes and atherosclerosis targets. During a drug-design program aiming at dual isoform-specific FABP4/5 inhibitors with little or no affinity for FABP3, a set of crystal structur…
View article: A high-resolution data set of fatty acid-binding protein structures. I. Dynamics of FABP4 and ligand binding
A high-resolution data set of fatty acid-binding protein structures. I. Dynamics of FABP4 and ligand binding Open
Fatty acid-binding proteins (FABPs) are involved in the uptake and intracellular trafficking of fatty acids for metabolic and gene-regulatory purposes. FABPs are known to associate with membranes and also enter the nucleus. Using NMR and a…
View article: A high-resolution data set of fatty acid-binding protein structures. III. Unexpectedly high occurrence of wrong ligands
A high-resolution data set of fatty acid-binding protein structures. III. Unexpectedly high occurrence of wrong ligands Open
FABP4 has been implicated as a therapeutic target for treating diabetes and atherosclerosis. Structure-based drug design (SBDD) based on initial hits from high-throughput and fragment screens yielded 216 ligand-bound structures of human FA…
View article: Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation
Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation Open
Fate decisions of T helper (Th) cells are tightly linked to their metabolic states, but precise mechanistic links remain unknown, especially in humans. Using in vitro stimulation in combination with gene editing we studied how metabolic re…
View article: Development of a Highly Selective NanoBRET Probe to Assess MAGL Inhibition in Live Cells
Development of a Highly Selective NanoBRET Probe to Assess MAGL Inhibition in Live Cells Open
Cell‐free enzymatic assays are highly useful tools in early compound profiling due to their robustness and scalability. However, their inadequacy to reflect the complexity of target engagement in a cellular environment may lead to a signif…
View article: A monoacylglycerol lipase inhibitor showing therapeutic efficacy in mice without central side effects or dependence
A monoacylglycerol lipase inhibitor showing therapeutic efficacy in mice without central side effects or dependence Open
Monoacylglycerol lipase (MAGL) regulates endocannabinoid 2-arachidonoylglycerol (2-AG) and eicosanoid signalling. MAGL inhibition provides therapeutic opportunities but clinical potential is limited by central nervous system (CNS)-mediated…
View article: P09.02 Modular P329G-binding CAR T cells engineered to recognize human effector-silenced antibodies carrying a P329G Fc-mutation for targeting of solid tumor antigens
P09.02 Modular P329G-binding CAR T cells engineered to recognize human effector-silenced antibodies carrying a P329G Fc-mutation for targeting of solid tumor antigens Open
Background Chimeric antigen receptor (CAR) T cell therapy revolutionized the treatment of patients with B cell and plasma cell malignancies. However, treatment success is still limited by treatment-associated toxicities and antigen-negativ…
View article: Chimeric antigen receptor T cells engineered to recognize the P329G-mutated Fc part of effector-silenced tumor antigen-targeting human IgG1 antibodies enable modular targeting of solid tumors
Chimeric antigen receptor T cells engineered to recognize the P329G-mutated Fc part of effector-silenced tumor antigen-targeting human IgG1 antibodies enable modular targeting of solid tumors Open
Background Chimeric antigen receptor (CAR) T cell therapy has proven its clinical utility in hematological malignancies. Optimization is still required for its application in solid tumors. Here, the lack of cancer-specific structures along…
View article: Synthesis, Characterization, and in vivo Evaluation of a Novel Potent Autotaxin-Inhibitor
Synthesis, Characterization, and in vivo Evaluation of a Novel Potent Autotaxin-Inhibitor Open
The autotaxin-lysophosphatidic acid (ATX-LPA) signaling pathway plays a role in a variety of autoimmune diseases, such as rheumatoid arthritis or neurodegeneration. A link to the pathogenesis of glaucoma is suggested by an overactive ATX-L…
View article: Variable heavy–variable light domain and Fab-arm CrossMabs with charged residue exchanges to enforce correct light chain assembly
Variable heavy–variable light domain and Fab-arm CrossMabs with charged residue exchanges to enforce correct light chain assembly Open
Technologies for the production of bispecific antibodies need to overcome two major challenges. The first one is correct heavy chain assembly, which was solved by knobs-into-holes technology or charge interactions in the CH3 domains. The s…
View article: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody Open
Invasion of erythrocytes by Plasmodial merozoites is a composite process involving the interplay of several proteins. Among them, the Plasmodium falciparum Cysteine-Rich Protective Antigen (PfCyRPA) is a crucial component of a ternary comp…
View article: MOESM6 of Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa
MOESM6 of Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa Open
Additional file 6. Compiled dataset of experimentally validated deletion strains with growth-deficient phenotypes compared to WT for genes in the plant cell wall degradation network (PCWDN) of N. crassa.
View article: MOESM2 of Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa
MOESM2 of Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa Open
Additional file 2. List of predicted genes coding for carbohydrate-active enzymes (CAZY) in N. crassa and the plant cell wall degradation network (PCWDN). List of CAZY genes in N. crassa was obtained from two sources: CAZY database [41] an…
View article: MOESM2 of The transcription factor PDR-1 is a multi-functional regulator and key component of pectin deconstruction and catabolism in Neurospora crassa
MOESM2 of The transcription factor PDR-1 is a multi-functional regulator and key component of pectin deconstruction and catabolism in Neurospora crassa Open
Additional file 2. Supplemental tables S1 â S8. Tables S1 and S2 contain the Venn diagrams of genes that were either 3-fold up- (S1) or down-regulated (S2) in the Î pdr-1 strain compared to the WT. The tables S3 and S4 show the FUNCAT anal…
View article: Author response: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody
Author response: Structure of the malaria vaccine candidate antigen CyRPA and its complex with a parasite invasion inhibitory antibody Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Invasion of erythrocytes by P…
View article: Hapten‐directed spontaneous disulfide shuffling: a universal technology for site‐directed covalent coupling of payloads to antibodies
Hapten‐directed spontaneous disulfide shuffling: a universal technology for site‐directed covalent coupling of payloads to antibodies Open
Humanized hapten‐binding IgGs were designed with an accessible cysteine close to their binding pockets, for specific covalent payload attachment. Individual analyses of known structures of digoxigenin (Dig)‐ and fluorescein (Fluo) binding …