Jorine M. Eeftens
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View article: Protocol to quantify mechanical properties of cells using optical tweezers
Protocol to quantify mechanical properties of cells using optical tweezers Open
View article: Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics
Heterogeneity as a feature: unraveling chromatin’s role in nuclear mechanics Open
Mechanical forces are a ubiquitous feature of the cellular environment. These forces propagate to the nucleus, where the mechanical response is critical for cellular function and survival. In addition to the nuclear lamina and cytoskeletal…
View article: Condensate-driven chromatin organization via elastocapillary interactions
Condensate-driven chromatin organization via elastocapillary interactions Open
Summary Biomolecular condensates are ubiquitous structures found throughout eukaryotic cells, with nuclear condensates playing a key role in the mesoscale organization and functionality of the genome 1,2 . Protein- and RNA-rich liquid-like…
View article: Robust quantification of cellular mechanics using optical tweezers
Robust quantification of cellular mechanics using optical tweezers Open
The mechanical properties of cells are closely related to function and play a crucial role in many cellular processes, including migration, differentiation, and cell fate determination. Numerous methods have been developed to assess cell m…
View article: Robust quantification of cellular mechanics using optical tweezers
Robust quantification of cellular mechanics using optical tweezers Open
Mechanical properties of cells are closely related to function and play a crucial role in many cellular processes, including migration, differentiation, and cell fate determination. Numerous methods have been developed to assess cell mecha…
View article: Interplay of condensation and chromatin binding underlies BRD4 targeting
Interplay of condensation and chromatin binding underlies BRD4 targeting Open
Nuclear compartments form via biomolecular phase separation, mediated through multivalent properties of biomolecules concentrated within condensates. Certain compartments are associated with specific chromatin regions, including transcript…
View article: Interplay of condensation and chromatin binding underlies BRD4 targeting
Interplay of condensation and chromatin binding underlies BRD4 targeting Open
Nuclear compartments form via biomolecular phase separation, mediated through multivalent properties of biomolecules concentrated within condensates. Certain compartments are associated with specific chromatin regions, including transcript…
View article: Viscoelasticity and advective flow of RNA underlies nucleolar form and function
Viscoelasticity and advective flow of RNA underlies nucleolar form and function Open
View article: CYSRT1: An Antimicrobial Epidermal Protein that Can Interact with Late Cornified Envelope Proteins
CYSRT1: An Antimicrobial Epidermal Protein that Can Interact with Late Cornified Envelope Proteins Open
Late cornified envelope (LCE) proteins are small cationic epidermal proteins with antimicrobial properties, and the combined deletion of LCE3B and LCE3C genes is a risk factor for psoriasis that affects skin microbiome composition. In a ye…
View article: Viscoelastic RNA entanglement and advective flow underlie nucleolar form and function
Viscoelastic RNA entanglement and advective flow underlie nucleolar form and function Open
The nucleolus facilitates transcription, processing, and assembly of ribosomal RNA (rRNA), the most abundant RNA in cells. Nucleolar function is facilitated by its multiphase liquid properties, but nucleolar fluidity and its connection to …
View article: Polycomb condensates can promote epigenetic marks but are not required for sustained chromatin compaction
Polycomb condensates can promote epigenetic marks but are not required for sustained chromatin compaction Open
Organization of the genome into transcriptionally active euchromatin and silenced heterochromatin is essential for eukaryotic cell function. Phase-separation has been implicated in heterochromatin formation, but it is unclear how phase-sep…
View article: Properties of repression condensates in living Ciona embryos
Properties of repression condensates in living Ciona embryos Open
Recent studies suggest that transcriptional activators and components of the pre-initiation complex (PIC) form higher order associations—clusters or condensates—at active loci. Considerably less is known about the distribution of repressor…
View article: Spatially Constrained Transcriptional Sources Drive Asphericity of Intracellular Liquids
Spatially Constrained Transcriptional Sources Drive Asphericity of Intracellular Liquids Open
View article: Epigenetic memory as a time integral over prior history of Polycomb phase separation
Epigenetic memory as a time integral over prior history of Polycomb phase separation Open
Structural organization of the genome into transcriptionally active euchromatin and silenced heterochromatin is essential for eukaryotic cell function. Heterochromatin is a more compact form of chromatin, and is associated with characteris…
View article: Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization
Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization Open
View article: Regulation of gene expression by repression condensates during development
Regulation of gene expression by repression condensates during development Open
There is emerging evidence for transcription condensates in the activation of gene expression 1–3 . However, there is considerably less information regarding transcriptional repression, despite its pervasive importance in regulating gene e…
View article: Distinct Roles for Condensin’s Two ATPase Sites in Chromosome Condensation
Distinct Roles for Condensin’s Two ATPase Sites in Chromosome Condensation Open
View article: High-Speed Atomic Force Microscopy of SMC Proteins
High-Speed Atomic Force Microscopy of SMC Proteins Open
View article: Real‐time detection of condensin‐driven <scp>DNA</scp> compaction reveals a multistep binding mechanism
Real‐time detection of condensin‐driven <span>DNA</span> compaction reveals a multistep binding mechanism Open
View article: The condensin complex is a mechanochemical motor that translocates along DNA
The condensin complex is a mechanochemical motor that translocates along DNA Open
Condensin is a highly processive DNA motor Condensin is thought to regulate genome architecture by creating DNA loops. Terakawa et al. used single-molecule imaging to show that yeast condensin is a highly processive mechanochemical motor c…
View article: Real-time detection of condensin-driven DNA compaction reveals a multistep binding mechanism
Real-time detection of condensin-driven DNA compaction reveals a multistep binding mechanism Open
Condensin, a conserved member of the SMC protein family of ring-shaped multi-subunit protein complexes, is essential for structuring and compacting chromosomes. Despite its key role, its molecular mechanism has remained largely unknown. He…
View article: The condensin complex is a mechanochemical motor that translocates along DNA
The condensin complex is a mechanochemical motor that translocates along DNA Open
One Sentence Summary Single-molecule imaging reveals that eukaryotic condensin is a highly processive DNA-translocating motor complex. Abstract Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic ba…
View article: Single-molecule approaches to unravel the mechanism of SMC proteins
Single-molecule approaches to unravel the mechanism of SMC proteins Open
Every cell deals with the challenge of organising its DNA. First, the DNA needs to be compacted in size by several orders of magnitude. For example, in each human cell, 2 meters of DNA need to fit inside a micron-sized cell nucleus. Second…
View article: Condensin Smc2-Smc4 Dimers Are Flexible and Dynamic
Condensin Smc2-Smc4 Dimers Are Flexible and Dynamic Open
View article: Single-Molecule Experiments to Resolve Structural and Mechanical Properties of Condensin
Single-Molecule Experiments to Resolve Structural and Mechanical Properties of Condensin Open
View article: Copper-free click chemistry for attachment of biomolecules in magnetic tweezers
Copper-free click chemistry for attachment of biomolecules in magnetic tweezers Open
This novel anchoring strategy using copper-free click chemistry allows to specifically and covalently link biomolecules, and conduct high-force single-molecule experiments. Excitingly, this advance opens up the possibility for single-molec…