José Hurst
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View article: Introducing a Porcine Inflammatory Ex Vivo Retina Model for Diabetic Retinopathy
Introducing a Porcine Inflammatory Ex Vivo Retina Model for Diabetic Retinopathy Open
This study aimed to develop an ex vivo retinal model to examine inflammatory processes in diabetic retinopathy (DR) without animal testing. Porcine eyes were collected from a local abattoir, dissected, and cultivated for four days in five …
View article: CORE SKILLS FOR MANUFACTURING WORKERS MSSC STANDARDS AND CERTIFICATION
CORE SKILLS FOR MANUFACTURING WORKERS MSSC STANDARDS AND CERTIFICATION Open
View article: Phosphorous-containing, amphiphilic ABB′ copolymers as siRNA nanocarriers with enhanced stability, reduced <i>in vitro</i> cytotoxicity, and efficient knockdown ability for the treatment of ocular diseases
Phosphorous-containing, amphiphilic ABB′ copolymers as siRNA nanocarriers with enhanced stability, reduced <i>in vitro</i> cytotoxicity, and efficient knockdown ability for the treatment of ocular diseases Open
Using phosphorus-containing, cationic polymers synthesized by REM-GTP, novel siRNA delivery agents were developed that demonstrate excellent siRNA encapsulation and efficient endosomal escape and gene silencing, while showing low cytotoxic…
View article: Effects of mesenchymal stromal cells and human recombinant Nerve Growth Factor delivered by bioengineered human corneal lenticule on an innovative model of diabetic retinopathy
Effects of mesenchymal stromal cells and human recombinant Nerve Growth Factor delivered by bioengineered human corneal lenticule on an innovative model of diabetic retinopathy Open
Introduction Diabetic retinopathy (DR) is a microvascular complication of diabetes in which neurodegeneration has been recently identified as a driving force. In the last years, mesenchymal stromal cells (MSCs) and neurotrophins like Nerve…
View article: Degradable and Biocompatible Magnesium Zinc Structures for Nanomedicine: Magnetically Actuated Liposome Microcarriers with Tunable Release
Degradable and Biocompatible Magnesium Zinc Structures for Nanomedicine: Magnetically Actuated Liposome Microcarriers with Tunable Release Open
Inorganic therapeutic carriers and implants should not only be biocompatible, but should also degrade under physiological conditions. Ideally, the time of the degradation can be controlled, and ideally the degradation products are fully bi…
View article: Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis
Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis Open
In the digital age, the widespread presence of electronic devices has exposed humans to an exceptional amount of blue light (BL) emitted from screens, LEDs, and other sources. Studies have shown that prolonged exposure to BL could have har…
View article: Co-cultivation of primary porcine RPE cells and neuroretina induces inflammation: a potential inflammatory AMD-model
Co-cultivation of primary porcine RPE cells and neuroretina induces inflammation: a potential inflammatory AMD-model Open
One common aspect in the pathology of many retinal diseases like age-related macular degeneration (AMD) is the death of retinal pigment epithelium (RPE) cells. RPE cells are essential for photoreceptor survival as they recycle and remove c…
View article: The Effect of mPEGA/EHA Ratio and Copolymer Composition on the Solution Behavior of Amphiphilic, Comb‐Shape Copolymers Synthesized via Cu(0)‐Mediated SET‐LRP for Potential Drug Delivery Applications
The Effect of mPEGA/EHA Ratio and Copolymer Composition on the Solution Behavior of Amphiphilic, Comb‐Shape Copolymers Synthesized via Cu(0)‐Mediated SET‐LRP for Potential Drug Delivery Applications Open
Comb‐shape, block copolymers from hydrophilic poly(ethylenglycol) monomethyl ether acrylate (mPEGA, A) and hydrophobic 2‐ethylhexyl acrylate (EHA, B) are synthesized by copper(0)‐mediated single‐electron transfer living radical polymerizat…
View article: A Case Study from the Past: “The RGC-5 vs. the 661W Cell Line: Similarities, Differences and Contradictions—Are They Really the Same?”
A Case Study from the Past: “The RGC-5 vs. the 661W Cell Line: Similarities, Differences and Contradictions—Are They Really the Same?” Open
In the pursuit of identifying the underlying pathways of ocular diseases, the use of cell lines such as (retinal ganglion cell-5) RGC-5 and 661W became a valuable tool, including pathologies like retinal degeneration and glaucoma. In 2001,…
View article: Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures Open
View article: Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells
Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells Open
In ophthalmic research, there is a strong need for in vitro corneal cell models. Here, we describe different protocols for the cultivation of primary corneal cells that were isolated from porcine eyes. This primary cell culture can be used…
View article: Editorial: Brain vs. retina - Differences and commonalities: The role of oxidative stress in neurodegenerative diseases
Editorial: Brain vs. retina - Differences and commonalities: The role of oxidative stress in neurodegenerative diseases Open
EDITORIAL article Front. Neurosci., 13 March 2023Sec. Neurodegeneration Volume 17 - 2023 | https://doi.org/10.3389/fnins.2023.1171235
View article: Lipid-DNA Nanoparticles as Drug-Delivery Vehicles for the Treatment of Retinal Diseases
Lipid-DNA Nanoparticles as Drug-Delivery Vehicles for the Treatment of Retinal Diseases Open
Retinal eye diseases are the leading cause of blindness in the Western world. Up to date, the only efficient treatment for many retinal diseases consists of invasive intravitreal injections of highly concentrated drugs. Despite the fact th…
View article: The function of p53 and its role in Alzheimer’s and Parkinson’s disease compared to age-related macular degeneration
The function of p53 and its role in Alzheimer’s and Parkinson’s disease compared to age-related macular degeneration Open
The protein p53 is the main human tumor suppressor. Since its discovery, extensive research has been conducted, which led to the general assumption that the purview of p53 is also essential for additional functions, apart from the preventi…
View article: Out of the Shadow: Blue Light Exposure Induces Apoptosis in Müller Cells
Out of the Shadow: Blue Light Exposure Induces Apoptosis in Müller Cells Open
Awareness toward the risks of blue light (BL) exposure is rising due to increased use of BL-enriched LEDs in displays. Short-wave BL (400–500 nm) has a high photochemical energy, leading to the enhanced production of reactive oxygen specie…
View article: Impact of Primary RPE Cells in a Porcine Organotypic Co-Cultivation Model
Impact of Primary RPE Cells in a Porcine Organotypic Co-Cultivation Model Open
The pathological events of age-related macular degeneration are characterized by degenerative processes involving the photoreceptor cells, retinal pigment epithelium (RPE), and the Bruch’s membrane as well as choroidal alterations. To mimi…
View article: Porcine Corneas Incubated at Low Humidity Present Characteristic Features Found in Dry Eye Disease
Porcine Corneas Incubated at Low Humidity Present Characteristic Features Found in Dry Eye Disease Open
Dry eye is a multifactorial disease that affects the ocular surface and tear fluid. Current treatment options include lubricant eye drop application several times a day. However, these eye drops often cause local side effects like ocular a…
View article: Long-Term Biocompatibility of a Highly Viscously Thiol-Modified Cross-Linked Hyaluronate as a Novel Vitreous Body Substitute
Long-Term Biocompatibility of a Highly Viscously Thiol-Modified Cross-Linked Hyaluronate as a Novel Vitreous Body Substitute Open
Purpose: In surgical ophthalmology, the treatment of complicated retinal and vitreous diseases is one of the central challenges. For this purpose, the vitreous body is removed as part of the standard therapy and replaced by a temporary tam…
View article: Topical Drug Delivery to the Posterior Segment of the Eye
Topical Drug Delivery to the Posterior Segment of the Eye Open
Topical drug delivery to the posterior segment of the eye is a very complex challenge. However, topical delivery is highly desired, to achieve an easy-to-use treatment option for retinal diseases. In this review, we focus on the drug chara…
View article: Cyclosporine A Protects Retinal Explants against Hypoxia
Cyclosporine A Protects Retinal Explants against Hypoxia Open
The retina is a complex neurological tissue and is extremely sensitive to an insufficient supply of oxygen. Hypoxia plays a major role in several retinal diseases, and often results in the loss of cells that are essential for vision. Cyclo…
View article: The expression of SARS-CoV 2-receptors in ocular tissue of normal and COVID-19 diseased patients
The expression of SARS-CoV 2-receptors in ocular tissue of normal and COVID-19 diseased patients Open
Purpose : To infect the eye, SARS-CoV 2 needs virus-specific receptors and coreceptors or proteases in the specific ocular tissue. The human angiotensin-converting enzyme-2 (ACE2) receptor represents the major gateway for SARS-CoV 2 to ent…
View article: Reduced Retinal Degeneration in an Oxidative Stress Organ Culture Model through an iNOS-Inhibitor
Reduced Retinal Degeneration in an Oxidative Stress Organ Culture Model through an iNOS-Inhibitor Open
In retinal organ cultures, H2O2 can be used to simulate oxidative stress, which plays a role in the development of several retinal diseases including glaucoma. We investigated whether processes underlying oxidative stress can be prevented …
View article: Can SARS-CoV-2 infect the eye? An overview of the receptor status in ocular tissue
Can SARS-CoV-2 infect the eye? An overview of the receptor status in ocular tissue Open
View article: Organ Cultures for Retinal Diseases
Organ Cultures for Retinal Diseases Open
The successful development of novel therapies is closely linked with understanding the underlying pathomechanisms of a disease. To do so, model systems that reflect human diseases and allow for the evaluation of new therapeutic approaches …
View article: Novel Porcine Retina Cultivation Techniques Provide Improved Photoreceptor Preservation
Novel Porcine Retina Cultivation Techniques Provide Improved Photoreceptor Preservation Open
Age-related macular degeneration (AMD) is the leading cause of blindness in industrialized countries among people over 60 years. It has multiple triggers and risk factors, but despite intense research efforts, its pathomechanisms are curre…
View article: Self-assembled DNA nanoparticles loaded with travoprost for glaucoma-treatment
Self-assembled DNA nanoparticles loaded with travoprost for glaucoma-treatment Open
View article: Kann SARS-CoV-2 das Auge infizieren? – Ein Überblick über den Rezeptorstatus in okularem Gewebe
Kann SARS-CoV-2 das Auge infizieren? – Ein Überblick über den Rezeptorstatus in okularem Gewebe Open
View article: Evaluation of nanorobots for targeted delivery into the retina
Evaluation of nanorobots for targeted delivery into the retina Open
View article: Reduced apoptosis and autophagy in an oxidative stress retina organ culture model through an iNOS-inhibitor
Reduced apoptosis and autophagy in an oxidative stress retina organ culture model through an iNOS-inhibitor Open
View article: iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model
iNOS‐inhibitor driven neuroprotection in a porcine retina organ culture model Open
Nitrite oxide plays an important role in the pathogenesis of various retinal diseases, especially when hypoxic processes are involved. This degeneration can be simulated by incubating porcine retinal explants with CoCl 2 . Here, the therap…