Josè Manuel Rodriguez-Martínez
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View article: Effect of pH on antimicrobial activity of delafloxacin against <i>Escherichia coli</i> isogenic strains carrying diverse chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms
Effect of pH on antimicrobial activity of delafloxacin against <i>Escherichia coli</i> isogenic strains carrying diverse chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms Open
The aim of this study is to evaluate the pH-dependent antimicrobial activities of delafloxacin against isogenic Escherichia coli strains carrying diverse fluoroquinolone resistance mechanisms. Eighty-one isogenic E. coli strains derived fr…
View article: Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens
Efficacy of the CHRONOlight biodynamic lighting system for control of nosocomial Gram-negative pathogens Open
CHRONOlight biodynamic lighting system is a novel broad-spectrum lighting prototype designed to effectively reduce the viability of gram-negative bacteria within short exposure times (30-300 seconds) through UV-C emission. This makes it a …
View article: Implications of gene expression heterogeneity in the interplay between acquired resistance and bacterial metabolism
Implications of gene expression heterogeneity in the interplay between acquired resistance and bacterial metabolism Open
View article: Combined inactivation of the SOS response with TCA fumarases and the adaptive response enhances antibiotic susceptibility against Escherichia coli
Combined inactivation of the SOS response with TCA fumarases and the adaptive response enhances antibiotic susceptibility against Escherichia coli Open
Introduction Targeting bacterial DNA damage responses such as the SOS response represents a promising strategy for enhancing the efficacy of existing antimicrobials. This study focuses on a recently discovered DNA damage response mechanism…
View article: P745: Congenital heart disease-associated coding and non-coding mutations alter TBX5-DNA binding affinity
P745: Congenital heart disease-associated coding and non-coding mutations alter TBX5-DNA binding affinity Open
View article: Heterogeneity of SOS response expression in clinical isolates of Escherichia coli influences adaptation to antimicrobial stress
Heterogeneity of SOS response expression in clinical isolates of Escherichia coli influences adaptation to antimicrobial stress Open
In recent years, new evidence has shown that the SOS response plays an important role in the response to antimicrobials, with involvement in the generation of clinical resistance. Here we evaluate the impact of heterogeneous expression of …
View article: Impact of suppression of the SOS response on protein expression in clinical isolates of Escherichia coli under antimicrobial pressure of ciprofloxacin
Impact of suppression of the SOS response on protein expression in clinical isolates of Escherichia coli under antimicrobial pressure of ciprofloxacin Open
Introduction/objective Suppression of the SOS response in combination with drugs damaging DNA has been proposed as a potential target to tackle antimicrobial resistance. The SOS response is the pathway used to repair bacterial DNA damage i…
View article: Abstract 1652 Investigating the DNA-binding specificity of cardiac transcription factors complexes
Abstract 1652 Investigating the DNA-binding specificity of cardiac transcription factors complexes Open
Transcription factors (TFs) are sequence-specific DNA-binding proteins responsible for cellular differentiation and development. Eukaryotic Transcription factors are known for binding DNA as multimeric complexes to regulate gene expression…
View article: Abstract 1489 Congenital Heart Disease-associated Mutations Alter TBX5-DNA Binding Affinity
Abstract 1489 Congenital Heart Disease-associated Mutations Alter TBX5-DNA Binding Affinity Open
Congenital heart diseases (CHDs) are the most common birth defect, affecting 1 in every 100 liveborn infants. CHDs are characterized by structural abnormalities that occur during heart development. Among the possible causes of CHDs are mut…
View article: Abstract 2446 Evaluation of DNA-binding preferences of human SIX family transcription factors using DAP-seq
Abstract 2446 Evaluation of DNA-binding preferences of human SIX family transcription factors using DAP-seq Open
Transcription factors (TFs) are proteins that bind to specific DNA sequences known as cis-regulatory elements (CREs). These proteins interact with the genome to regulate gene expression, making them essential for virtually every cellular p…
View article: Abstract 1638 Bioinformatic Prioritization of Cardiovascular Disease-Linked Variants Modulating TBX5 Binding
Abstract 1638 Bioinformatic Prioritization of Cardiovascular Disease-Linked Variants Modulating TBX5 Binding Open
Cardiovascular diseases (CVDs) are the leading cause of death worldwide, responsible for approximately 17.9 million lives each year. Genome-wide association studies (GWAS) have linked multiple types of CVDs with mutations, specifically wit…
View article: Evaluation of temocillin efficacy against KPC-2-producing <i>Klebsiella pneumoniae</i> isolates in a hollow-fibre infection model
Evaluation of temocillin efficacy against KPC-2-producing <i>Klebsiella pneumoniae</i> isolates in a hollow-fibre infection model Open
Background Temocillin is an old antimicrobial that is resistant to hydrolysis by ESBLs but has variable activity against carbapenemase-producing Enterobacteriaceae. The current EUCAST susceptibility breakpoints for Enterobacterales are set…
View article: Implications of two-component systems <i>EnvZ</i>/<i>OmpR</i> and <i>BaeS</i>/<i>BaeR</i> in <i>in vitro</i> temocillin resistance in <i>Escherichia coli</i>
Implications of two-component systems <i>EnvZ</i>/<i>OmpR</i> and <i>BaeS</i>/<i>BaeR</i> in <i>in vitro</i> temocillin resistance in <i>Escherichia coli</i> Open
Background BaeS/BaeR is a two-component system of Escherichia coli that controls the expression of porins and efflux pumps. Its role in beta-lactam resistance is limited. Objectives To study the role of baeS/baeR two-component system in te…
View article: The <i>mef</i> (A)/ <i>msr</i> (D)-carrying streptococcal prophage Φ1207.3 encodes an SOS-like system, induced by UV-C light, responsible for increased survival and increased mutation rate
The <i>mef</i> (A)/ <i>msr</i> (D)-carrying streptococcal prophage Φ1207.3 encodes an SOS-like system, induced by UV-C light, responsible for increased survival and increased mutation rate Open
Bacterial SOS response is an inducible system of DNA repair and mutagenesis. Streptococci lack a canonical SOS response, but an SOS-like response was reported in some species. The mef (A)- msr (D)-carrying prophage Ф1207.3 of Streptococcus…
View article: Synergistic Effect of SOS Response and GATC Methylome Suppression on Antibiotic Stress Survival in Escherichia coli
Synergistic Effect of SOS Response and GATC Methylome Suppression on Antibiotic Stress Survival in Escherichia coli Open
The suppression of the SOS response has been shown to enhance the in vitro activity of quinolones. Furthermore, Dam-dependent base methylation has an impact on susceptibility to other antimicrobials affecting DNA synthesis.
View article: Effect of Glycerol on Fosfomycin Activity against Escherichia coli
Effect of Glycerol on Fosfomycin Activity against Escherichia coli Open
Fosfomycin is an antimicrobial that inhibits the biosynthesis of peptidoglycan by entering the bacteria through two channels (UhpT and GlpT). Glycerol is clinically used as a treatment for elevated intracranial pressure and induces the exp…
View article: Prevalence, Incidence, and Risk Factors for Intestinal Colonization Due to Fluoroquinolone-Resistant ST131 Escherichia coli: a Longitudinal Study in Highly Dependent, Long-Term Care Facility Residents
Prevalence, Incidence, and Risk Factors for Intestinal Colonization Due to Fluoroquinolone-Resistant ST131 Escherichia coli: a Longitudinal Study in Highly Dependent, Long-Term Care Facility Residents Open
We aimed at investigating the microbiological and epidemiological features of clade C fluoroquinolone-resistant ST131 E. coli isolates colonizing highly dependent residents in long-term care facilities (LTCFs) during 40 weeks and the risk …
View article: Potential clinical significance of statins on methicillin resistance reversion in Staphylococcus aureus
Potential clinical significance of statins on methicillin resistance reversion in Staphylococcus aureus Open
View article: Interplay between IncF plasmids and topoisomerase mutations conferring quinolone resistance in the Escherichia coli ST131 clone: stability and resistance evolution
Interplay between IncF plasmids and topoisomerase mutations conferring quinolone resistance in the Escherichia coli ST131 clone: stability and resistance evolution Open
The Escherichia coli ST131 H30 -Rx subclone vehicles CTX-M-15 plasmids and mutations in gyrA and parC conferring multidrug resistance successfully in the clinical setting. The aim of this study was (1) to investigate the relationship of sp…
View article: Effect of RecA inactivation and detoxification systems on the evolution of ciprofloxacin resistance in <i>Escherichia coli</i>
Effect of RecA inactivation and detoxification systems on the evolution of ciprofloxacin resistance in <i>Escherichia coli</i> Open
Background Suppression of SOS response and overproduction of reactive oxygen species (ROS) through detoxification system suppression enhance the activity of fluoroquinolones. Objectives To evaluate the role of both systems in the evolution…
View article: Clinical characteristics and outcome of bacteraemia caused by Enterobacter cloacae and Klebsiella aerogenes: more similarities than differences
Clinical characteristics and outcome of bacteraemia caused by Enterobacter cloacae and Klebsiella aerogenes: more similarities than differences Open
View article: Disbalancing Envelope Stress Responses as a Strategy for Sensitization of Escherichia coli to Antimicrobial Agents
Disbalancing Envelope Stress Responses as a Strategy for Sensitization of Escherichia coli to Antimicrobial Agents Open
Disbalancing envelope stress responses was investigated as a strategy for sensitization of Escherichia coli to antimicrobial agents. Seventeen isogenic strains were selected from the KEIO collection with deletions in genes corresponding to…
View article: Activity of Fosfomycin and Amikacin against Fosfomycin-Heteroresistant <i>Escherichia coli</i> Strains in a Hollow-Fiber Infection Model
Activity of Fosfomycin and Amikacin against Fosfomycin-Heteroresistant <i>Escherichia coli</i> Strains in a Hollow-Fiber Infection Model Open
We evaluated human-like the efficacy of intravenous doses of fosfomycin of 8 g every 8 h (8 g/Q8h) and of amikacin (15 mg/kg/Q24h) in monotherapy and in combination against six fosfomycin-heteroresistant Escherichia coli isolates using a h…
View article: Interplay among Different Fosfomycin Resistance Mechanisms in Klebsiella pneumoniae
Interplay among Different Fosfomycin Resistance Mechanisms in Klebsiella pneumoniae Open
The objectives of this study were to characterize the role of the uhpT , glpT , and fosA genes in fosfomycin resistance in Klebsiella pneumoniae and evaluate the use of sodium phosphonoformate (PPF) in combination with fosfomycin. Seven cl…
View article: Synergistic Quinolone Sensitization by Targeting the <i>recA</i> SOS Response Gene and Oxidative Stress
Synergistic Quinolone Sensitization by Targeting the <i>recA</i> SOS Response Gene and Oxidative Stress Open
Suppression of the recA SOS response gene and reactive oxygen species (ROS) overproduction have been shown, separately, to enhance fluoroquinolone activity and lethality. Their putative synergistic impact as a strategy to potentiate the ef…
View article: Detection of Low-Level Fosfomycin-Resistant Variants by Decreasing Glucose-6-Phosphate Concentration in Fosfomycin Susceptibility Determination
Detection of Low-Level Fosfomycin-Resistant Variants by Decreasing Glucose-6-Phosphate Concentration in Fosfomycin Susceptibility Determination Open
Mutations that confer low-level fosfomycin resistance (LLFR) but not clinical resistance in Escherichia coli are increasingly reported. LLFR strains can become clinically resistant under urinary tract physiological conditions or may act as…
View article: Role of low-level quinolone resistance in generating tolerance in Escherichia coli under therapeutic concentrations of ciprofloxacin
Role of low-level quinolone resistance in generating tolerance in Escherichia coli under therapeutic concentrations of ciprofloxacin Open
Background Tolerance (including persistence) and resistance result in increased survival under antibiotic pressure. Objectives We evaluated the interplay between resistance and tolerance to ciprofloxacin under therapeutic and killing condi…
View article: Suppression of the SOS response modifies spatiotemporal evolution, post-antibiotic effect, bacterial fitness and biofilm formation in quinolone-resistant Escherichia coli
Suppression of the SOS response modifies spatiotemporal evolution, post-antibiotic effect, bacterial fitness and biofilm formation in quinolone-resistant Escherichia coli Open
Our data indicate that suppression of the SOS response affects key bacterial traits and is a promising strategy for reversing and tackling the evolution of antibiotic resistance in E. coli, including low-level and resistant phenotypes at t…
View article: Urinary Tract Conditions Affect Fosfomycin Activity against Escherichia coli Strains Harboring Chromosomal Mutations Involved in Fosfomycin Uptake
Urinary Tract Conditions Affect Fosfomycin Activity against Escherichia coli Strains Harboring Chromosomal Mutations Involved in Fosfomycin Uptake Open
The steps by which Escherichia coli strains harboring mutations related to fosfomycin (FOS) resistance arise and spread during urinary tract infections (UTIs) are far from being understood. The aim of this study was to evaluate the effects…
View article: Quinolone Resistance Reversion by Targeting the SOS Response
Quinolone Resistance Reversion by Targeting the SOS Response Open
Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichi…