Explanipedia

Gromomycins: An Unprecedented Class of Triterpene Antibiotics Produced by a Novel Biosynthetic Pathway Open

Andriy Luzhetskyy, Stepan Tistechok, Dmytro Bratiichuk, Hilda Sucipto, Nils Gummerlich , et al. · 2025

The current situation with drug‐resistant microbial pathogens is critical, dictating an acute need for novel efficient antibiotics. Herein, we report a new class of antibiotics named gromomycins with significant activity, especially agains…

Gromomycins: An Unprecedented Class of Triterpene Antibiotics Produced by a Novel Biosynthetic Pathway Open

Andriy Luzhetskyy, Stepan Tistechok, Dmytro Bratiichuk, Hilda Sucipto, Nils Gummerlich , et al. · 2025

The current situation with drug‐resistant microbial pathogens is critical, dictating an acute need for novel efficient antibiotics. Herein, we report a new class of antibiotics named gromomycins with significant activity, especially agains…

Characterization and Metabolism of Drug Products Containing the Cocaine-Like New Psychoactive Substances Indatraline and Troparil Open

Sascha K. Manier, Paula Mumber, Josef Zapp, Niels Eckstein, Markus R. Meyer · 2024

With a rising demand of cocaine over the last years, it is likely that unregulated new psychoactive substances with similar effects such as indatraline ((1R,3S)-3-(3,4-dichlorophenyl)-N-methyl-2,3-dihydro-1H-inden-1-amine) and troparil (Me…

Discovery and overproduction of novel highly bioactive pamamycins through transcriptional engineering of the biosynthetic gene cluster Open

Nikolas Eckert, Yuriy Rebets, Lilya Horbal, Josef Zapp, Jennifer Herrmann , et al. · 2023

Heterologous Production and Biosynthesis of Threonine-16:0dioic acids with a Hydroxamate Moiety Open

Marc Stierhof, Maksym Myronovskyi, Josef Zapp, Andriy Luzhetskyy · 2023

Dereplication and genome mining in Streptomyces aureus LU18118 combined with heterologous expression of selected biosynthetic gene clusters (BGCs) led to the discovery of various threonine-16:0dioic acids named lipothrenins. Lipothr…

Dynamics, cation conformation and rotamers in guanidinium ionic liquids with ether groups Open

Daniel Rauber, Frederik Philippi, Bernd Morgenstern, Josef Zapp, Björn Kuttich , et al. · 2023

The calcium channel modulator 2-APB hydrolyzes in physiological buffers and acts as an effective radical scavenger and inhibitor of the NADPH oxidase 2 Open

Ewa Jasmin Slowik, Katerina Stankoska, Nhat Nguyen Bui, Bastian Pasieka, David Conrad , et al. · 2023

CCDC 2157463: Experimental Crystal Structure Determination Open

Daniel Rauber, Frederik Philippi, Julian Becker, Josef Zapp, Bernd Morgenstern , et al. · 2023

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …

CCDC 2157466: Experimental Crystal Structure Determination Open

Daniel Rauber, Frederik Philippi, Julian Becker, Josef Zapp, Bernd Morgenstern , et al. · 2023

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …

Dynamics, Cation Conformation and Rotamers in Guandinium Ionic Liquids with Ether Groups Open

Daniel Rauber, Frederik Philippi, Bernd Morgenstern, Josef Zapp, Björn Kuttich , et al. · 2023

Anion and ether group influence in protic guanidinium ionic liquids Open

Daniel Rauber, Frederik Philippi, Julian Becker, Josef Zapp, Bernd Morgenstern , et al. · 2023

Dynamics and structures of protic ionic liquids from the superbase guanidinium are analyzed in detail. The H-bond strength correlates with the proton affinity of the constituting acid and can be monitored by the 1 H or 15 N chemical shifts.

Furaquinocins K and L: Novel Naphthoquinone-Based Meroterpenoids from Streptomyces sp. Je 1-369 Open

Stepan Tistechok, Marc Stierhof, Maksym Myronovskyi, Josef Zapp, Oleksandr Gromyko , et al. · 2022

Actinomycetes are the most prominent group of microorganisms that produce biologically active compounds. Among them, special attention is focused on bacteria in the genus Streptomyces. Streptomycetes are an important source of biologically…

Miramides A–D: Identification of Detoxin-like Depsipeptides after Heterologous Expression of a Hybrid NRPS-PKS Gene Cluster from Streptomyces mirabilis Lu17588 Open

Constanze Paulus, Maksym Myronovskyi, Josef Zapp, Marta Rodríguez Estévez, Maria Lopatniuk , et al. · 2022

Natural products derived from plants, fungi or bacteria have been used for years in the medicine, agriculture and food industries as they exhibit a variety of beneficial properties, such as antibiotic, antifungal, anticancer, herbicidal an…

New Scabimycins A-C Isolated from Streptomyces acidiscabies (Lu19992) Open

Constanze Paulus, Josef Zapp, Andriy Luzhetskyy · 2021

Peptide natural products displaying a wide range of biological activities have become important drug candidates over the years. Microorganisms have been a powerful source of such bioactive peptides, and Streptomyces have yielded many novel…

Bonsecamin: A New Cyclic Pentapeptide Discovered through Heterologous Expression of a Cryptic Gene Cluster Open

Constanze Lasch, Marc Stierhof, Marta Rodríguez Estévez, Maksym Myronovskyi, Josef Zapp , et al. · 2021

The intriguing structural complexity of molecules produced by natural organisms is uncontested. Natural scaffolds serve as an important basis for the development of molecules with broad applications, e.g., therapeutics or agrochemicals. Re…

Cyclofaulknamycin with the Rare Amino Acid D-capreomycidine Isolated from a Well-Characterized Streptomyces albus Strain Open

Liliya Horbal, Marc Stierhof, Anja Palusczak, Nikolas Eckert, Josef Zapp , et al. · 2021

Targeted genome mining is an efficient method of biosynthetic gene cluster prioritization within constantly growing genome databases. Using two capreomycidine biosynthesis genes, alpha-ketoglutarate-dependent arginine beta-hydroxylase and …

Discovery and Heterologous Production of New Cyclic Depsibosamycins Open

Marc Stierhof, Maksym Myronovskyi, Josef Zapp, Andriy Luzhetskyy · 2021

Streptomyces are producers of valuable secondary metabolites with unique scaffolds that perform a plethora of biological functions. Nonribosomal peptides are of special interest due to their variety and complexity. They are synthesized by …

Targeted Genome Mining—From Compound Discovery to Biosynthetic Pathway Elucidation Open

Nils Gummerlich, Yuriy Rebets, Constanze Paulus, Josef Zapp, Andriy Luzhetskyy · 2020

Natural products are an important source of novel investigational compounds in drug discovery. Especially in the field of antibiotics, Actinobacteria have been proven to be a reliable source for lead structures. The discovery of these natu…

Dudomycins: New Secondary Metabolites Produced after Heterologous Expression of an Nrps Cluster from Streptomyces albus ssp. Chlorinus Nrrl B-24108 Open

Constanze Lasch, Marc Stierhof, Marta Rodríguez Estévez, Maksym Myronovskyi, Josef Zapp , et al. · 2020

Since the 1950s, natural products of bacterial origin were systematically developed to be used as drugs with a wide range of medical applications. The available treatment options for many diseases are still not satisfying, wherefore, the d…

Loseolamycins: A Group of New Bioactive Alkylresorcinols Produced after Heterologous Expression of a Type III PKS from Micromonospora endolithica Open

Constanze Lasch, Nils Gummerlich, Maksym Myronovskyi, Anja Palusczak, Josef Zapp , et al. · 2020

Natural products are a valuable source of biologically active compounds with potential applications in medicine and agriculture. Unprecedented scaffold diversity of natural products and biocatalysts from their biosynthetic pathways are of …

Rapid Organocatalytic Formation of Carbon Monoxide: Application towards Carbonylative Cross Couplings Open

Ben G. E. Zoller, Josef Zapp, Peter Huy · 2020

Herein, the first organocatalytic method for the transformation of non‐derivatized formic acid into carbon monoxide (CO) is introduced. Formylpyrrolidine (FPyr) and trichlorotriazine (TCT), which is a cost‐efficient commodity chemical, ena…

Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A Open

Irina Voitsekhovskaia, Constanze Paulus, Charlotte Dahlem, Yuriy Rebets, Suvd Nadmid , et al. · 2020

Natural products produced by bacteria found in unusual and poorly studied ecosystems, such as Lake Baikal, represent a promising source of new valuable drug leads. Here we report the isolation of a new Streptomyces sp. strain IB201691-2A f…

Benzanthric Acid, a Novel Metabolite From Streptomyces albus Del14 Expressing the Nybomycin Gene Cluster Open

Marta Rodríguez Estévez, Nils Gummerlich, Maksym Myronovskyi, Josef Zapp, Andriy Luzhetskyy · 2020

Streptomycetes constitute a diverse bacterial group able to produce a wide variety of secondary metabolites with potential applications in the pharmacy industry. However, the genes responsible for the biosynthesis of these compounds are ve…

Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters Open

Yousra Ahmed, Yuriy Rebets, Marta Rodríguez Estévez, Josef Zapp, Maksym Myronovskyi , et al. · 2020

Background Heterologous expression of secondary metabolite gene clusters is used to achieve increased production of desired compounds, activate cryptic gene clusters, manipulate clusters from genetically unamenable strains, obtain natural …

Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs Open

Abdul Baseer, Aljoscha Koenneke, Josef Zapp, Saeed Ahmad Khan, Marc Schneider · 2019

For nanotechnology enabled delivery of hydrophilic protein‐based drugs, several polymer‐based carrier systems have been used in the past to protect the sensitive load and to facilitate cellular uptake and crossing of biological barriers. T…

Activity-guided purification reveals quercetin as the most efficient cholinesterase inhibitor in wild strawberry (Fragaria vesca L.) and apricot (Prunus armeniaca L.) fruit extract Open

Kamila Borowiec Dominik Szwajgier, Josef Zapp · 2019

Cholinesterase (ChE) inhibitors are commonly approved for the Alzheimer’s disease therapy which should restore the original levels of acetylcholine in the brain. In this work, a suitable purification procedure of ChE inhibitors from wild s…

New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme Open

Constanze Paulus, Yuriy Rebets, Josef Zapp, Christian Rückert, Jörn Kalinowski , et al. · 2018

The environment of Lake Baikal is a well-known source of microbial diversity. The strain Streptomyces sp. IB2014/011-12, isolated from samples collected at Lake Baikal, was found to exhibit potent activity against Gram-positive bact…

Transport properties of protic and aprotic guanidinium ionic liquids Open

Daniel Rauber, Frederik Philippi, Josef Zapp, Guido Kickelbick, Harald Natter , et al. · 2018

New protic and aprotic ionic liquids based on superbase cations show promising properties and enrich the field of cation classes

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase Open

Ammar Abdulmughni, I.K. Jozwik, Natalia Putkaradze, Elisa Brill, Josef Zapp , et al. · 2016

Structural basis of steroid binding and oxidation by the cytochrome P450 <span>CYP</span>109E1 from <i>Bacillus megaterium</i> Open

I.K. Jozwik, Flora Marta Kiss, Łukasz Gricman, Ammar Abdulmughni, Elisa Brill , et al. · 2016

Cytochrome P450 monooxygenases (P450s) are attractive enzymes for the pharmaceutical industry, in particular, for applications in steroidal drug synthesis. Here, we report a comprehensive functional and structural characterization of CYP 1…

Josef Zapp YOU? Author Swipe