Joseph Hardie
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View article: Automated, Deep Learning‐Enabled Immunoassay Microfluidic Cartridge for Viral Pathogen Detection
Automated, Deep Learning‐Enabled Immunoassay Microfluidic Cartridge for Viral Pathogen Detection Open
Enzyme‐linked immunosorbent assays (ELISA) are recognized for their accuracy and versatility in disease diagnostics. However, conventional ELISA protocols rely on laboratory equipment, trained personnel, and established infrastructure, res…
View article: A fully automated, ultrasensitive luminescence cascade sensor to address hepatitis C diagnostic disparity
A fully automated, ultrasensitive luminescence cascade sensor to address hepatitis C diagnostic disparity Open
Viral hepatitis poses a significant global health burden, with chronic hepatitis B and C causing about 1 million annual deaths from liver cancer and cirrhosis. Over 1.5 million new hepatitis C virus (HCV) cases arise yearly, especially amo…
View article: Reducing hepatitis C diagnostic disparities with a fully automated deep learning–enabled microfluidic system for HCV antigen detection
Reducing hepatitis C diagnostic disparities with a fully automated deep learning–enabled microfluidic system for HCV antigen detection Open
Viral hepatitis remains a major global health issue, with chronic hepatitis B (HBV) and hepatitis C (HCV) causing approximately 1 million deaths annually, primarily due to liver cancer and cirrhosis. More than 1.5 million people contract H…
View article: Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay
Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay Open
Digital nucleic acid assays, known for their high sensitivity and specificity, typically rely on fluorescent readouts and expensive and complex nanowell manufacturing, which constrain their broader use in point-of-care (POC) application. H…
View article: Long‐Lasting, Transparent Antibacterial Shield: A Durable, Broad‐Spectrum Anti‐Bacterial, Non‐Cytotoxic, Transparent Nanocoating for Extended Wear Contact Lenses
Long‐Lasting, Transparent Antibacterial Shield: A Durable, Broad‐Spectrum Anti‐Bacterial, Non‐Cytotoxic, Transparent Nanocoating for Extended Wear Contact Lenses Open
The increasing incidence of serious bacterial keratitis, a sight‐threatening condition often exacerbated by inadequate contact lens (CLs) care, highlights the need for innovative protective technology. This study introduces a long‐lasting …
View article: Selective treatment of intracellular bacterial infections using host cell-targeted bioorthogonal nanozymes
Selective treatment of intracellular bacterial infections using host cell-targeted bioorthogonal nanozymes Open
Macrophage-targeted bioorthogonal nanozyme designed to generate antibiotics at the site of infection. Nanozymes had targeted uptake by macrophages through mannose functionalization, then generated antibiotics to specifically kill infectiou…
View article: Deep learning-assisted sensitive detection of fentanyl using a bubbling-microchip
Deep learning-assisted sensitive detection of fentanyl using a bubbling-microchip Open
Deep learning-enabled smartphone-based image processing has significant advantages in the development of point-of-care diagnostics.
View article: SARS‐CoV‐2 RNA Detection by a Cellphone‐Based Amplification‐Free System with CRISPR/CAS‐Dependent Enzymatic (CASCADE) Assay
SARS‐CoV‐2 RNA Detection by a Cellphone‐Based Amplification‐Free System with CRISPR/CAS‐Dependent Enzymatic (CASCADE) Assay Open
CRISPR (Clustered regularly interspaced short palindromic repeats)‐based diagnostic technologies have emerged as a promising alternative to accelerate delivery of SARS‐CoV‐2 molecular detection at the point of need. However, efficient tran…
View article: CHEMICAL MANIPULATION OF MACROPHAGES: NANOMATERIAL AND MOLECULAR APPROACHES
CHEMICAL MANIPULATION OF MACROPHAGES: NANOMATERIAL AND MOLECULAR APPROACHES Open
Macrophages, phagocytic cells of the innate immune system, are the body’s first line of defense against pathogens and are responsible for tissue maintenance. Macrophages are capable of sensing and internalizing external stimuli, and in res…
View article: Nanodelivery vehicles induce remote biochemical changes<i>in vivo</i>
Nanodelivery vehicles induce remote biochemical changes<i>in vivo</i> Open
Dual-mode mass spectrometry imaging shows that the biochemical effects of anti-TNF-α nanoparticle stabilized capsules occur in regions that are spatially remote from where the nanomaterials accumulate.
View article: High-content and high-throughput identification of macrophage polarization phenotypes
High-content and high-throughput identification of macrophage polarization phenotypes Open
Phenotyping macrophage activation states using an array-based sensor. FRET complex assembly selectively interacts with the macrophage surface, generating a fingerprint for each polarization state that is further used to identify the activa…
View article: Dual Mass Spectrometric Tissue Imaging of Nanocarrier Distributions and Their Biochemical Effects
Dual Mass Spectrometric Tissue Imaging of Nanocarrier Distributions and Their Biochemical Effects Open
Nanomaterial-based drug delivery vehicles are able to deliver therapeutics in a controlled, targeted manner. Currently, however, there are limited analytical methods that can detect both nanomaterial distributions and their biochemical eff…
View article: In Vivo Editing of Macrophages through Systemic Delivery of CRISPR‐Cas9‐Ribonucleoprotein‐Nanoparticle Nanoassemblies
In Vivo Editing of Macrophages through Systemic Delivery of CRISPR‐Cas9‐Ribonucleoprotein‐Nanoparticle Nanoassemblies Open
Macrophages are key effectors of host defense and metabolism, making them promising targets for transient genetic therapy. Gene editing through the delivery of Cas9‐ribonucleoprotein (RNP) provides multiple advantages over gene delivery–ba…
View article: Macrophage Activation by a Substituted Pyrimido[5,4-<i>b</i>]Indole Increases Anti-Cancer Activity
Macrophage Activation by a Substituted Pyrimido[5,4-<i>b</i>]Indole Increases Anti-Cancer Activity Open
Immunotherapy has become a promising new approach for cancer treatment due to the immune system’s ability to remove tumors in a safe and specific manner. Many tumors express anti-inflammatory factors that deactivate the local immune respon…
View article: Correction to “Surface-Modified Macrophages Facilitate Tracking of Breast Cancer-Immune Interactions”
Correction to “Surface-Modified Macrophages Facilitate Tracking of Breast Cancer-Immune Interactions” Open
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "Surface-Modified Macrophages Facilitate Tracking of Breast Cancer-Immune Interactions"Bishnu P. JoshiBishnu P. JoshiMore by B…
View article: Frontispiece: Harnessing Biology to Deliver Therapeutic and Imaging Entities via Cell‐Based Methods
Frontispiece: Harnessing Biology to Deliver Therapeutic and Imaging Entities via Cell‐Based Methods Open
Delivery systems based on living cells have recently gained much recognition, with several in clinical trials. While traditional vehicles rely on passive accumulation at sites of interest and once there, can encounter issues associated wit…
View article: Surface-Modified Macrophages Facilitate Tracking of Breast Cancer-Immune Interactions
Surface-Modified Macrophages Facilitate Tracking of Breast Cancer-Immune Interactions Open
The immune system has been found to play key roles in cancer development and progression. Macrophages are typically considered to be pro-inflammatory cells but can also facilitate prooncogenic activities via associations with tumors and me…
View article: Harnessing Biology to Deliver Therapeutic and Imaging Entities via Cell‐Based Methods
Harnessing Biology to Deliver Therapeutic and Imaging Entities via Cell‐Based Methods Open
The accumulation of therapeutic and imaging agents at sites of interest is critical to their efficacy. Similarly, off‐target effects (especially toxicity) are a major liability for these entities. For this reason, the use of delivery vehic…
View article: Protein delivery into cells using inorganic nanoparticle–protein supramolecular assemblies
Protein delivery into cells using inorganic nanoparticle–protein supramolecular assemblies Open
Direct intracellular delivery of proteins using inorganic nanoparticle–protein supramolecular assemblies.
View article: Simultaneous cytosolic delivery of a chemotherapeutic and siRNA using nanoparticle-stabilized nanocapsules
Simultaneous cytosolic delivery of a chemotherapeutic and siRNA using nanoparticle-stabilized nanocapsules Open
We report on nanoparticle-stabilized capsules (NPSCs) as a platform for the co-delivery of survivin-targeted siRNA and tamoxifen. These capsules feature an inner oil core that provides a carrier for tamoxifen, and is coated on the surface …
View article: Chemically Engineered Nanoparticle–Protein Interface for Real‐Time Cellular Oxidative Stress Monitoring
Chemically Engineered Nanoparticle–Protein Interface for Real‐Time Cellular Oxidative Stress Monitoring Open
A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-…
View article: Progress and perspective of inorganic nanoparticle-based siRNA delivery systems
Progress and perspective of inorganic nanoparticle-based siRNA delivery systems Open
Several challenges remain in developing inorganic NPs for efficient and effective siRNA delivery. Getting the material to the chosen site is important, however the greatest hurdle may well be delivery into the cytosol, either through effic…