Joseph M. Miano
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View article: Development and characterization of a fully humanized ACE2 mouse model
Development and characterization of a fully humanized ACE2 mouse model Open
The hACE2 model offers a more accurate approach to studying mechanisms underlying tissue-restricted expression of ACE2, elucidating noncoding sequence variants and an upstream LncRNA, and defining pathways relevant to human disease and ass…
View article: Activation of receptor-independent fluid-phase pinocytosis promotes foamy monocyte formation in atherosclerotic mice
Activation of receptor-independent fluid-phase pinocytosis promotes foamy monocyte formation in atherosclerotic mice Open
Atherosclerotic cardiovascular disease (ASCVD) is the leading cause of death worldwide. Clinical and experimental data demonstrated that circulating monocytes internalize plasma lipoproteins and become lipid-laden foamy cells in hyperchole…
View article: Omicron XBB.1.5 subvariant causes severe pulmonary disease in K18-hACE-2 mice
Omicron XBB.1.5 subvariant causes severe pulmonary disease in K18-hACE-2 mice Open
Owing to their continuous evolution, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) display disparate pathogenicity in mouse models. Omicron and its sublineages have been dominant worldwide. Compare…
View article: <i> G6pd <sup>N126D</sup> </i> Variant Increases the Risk of Developing VEGFR (Vascular Endothelial Growth Factor Receptor) Blocker‐Induced Pulmonary Vascular Disease
<i> G6pd <sup>N126D</sup> </i> Variant Increases the Risk of Developing VEGFR (Vascular Endothelial Growth Factor Receptor) Blocker‐Induced Pulmonary Vascular Disease Open
Background G6PD (glucose‐6‐phosphate‐dehydrogenase) is a key enzyme in the glycolytic pathway and has been implicated in the pathogenesis of cancer and pulmonary hypertension‐associated vascular remodeling. Here, we investigated the role o…
View article: Prime editing in mice with an engineered pegRNA
Prime editing in mice with an engineered pegRNA Open
View article: Calponin 1 inhibits <scp>agonist‐induced ERK</scp> activation and decreases calcium sensitization in vascular smooth muscle
Calponin 1 inhibits <span>agonist‐induced ERK</span> activation and decreases calcium sensitization in vascular smooth muscle Open
Smooth muscle cell (SMC) contraction and vascular tone are modulated by phosphorylation and multiple modifications of the thick filament, and thin filament regulation of SMC contraction has been reported to involve extracellular regulated …
View article: HDL regulates TGFß-receptor lipid raft partitioning, restoring contractile features of cholesterol-loaded vascular smooth muscle cells
HDL regulates TGFß-receptor lipid raft partitioning, restoring contractile features of cholesterol-loaded vascular smooth muscle cells Open
Background Cholesterol-loading of mouse aortic vascular smooth muscle cells (mVSMCs) downregulates miR-143/145 , a master regulator of the contractile state downstream of TGFβ signaling. In vitro, this results in transitioning from a contr…
View article: Author Correction: Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells
Author Correction: Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells Open
View article: A New “Lnc” to Brake Inflammation
A New “Lnc” to Brake Inflammation Open
View article: <i>INKILN</i> is a Novel Long Noncoding RNA Promoting Vascular Smooth Muscle Inflammation via Scaffolding MKL1 and USP10
<i>INKILN</i> is a Novel Long Noncoding RNA Promoting Vascular Smooth Muscle Inflammation via Scaffolding MKL1 and USP10 Open
Background: Activation of vascular smooth muscle cell (VSMC) inflammation is vital to initiate vascular disease. The role of human-specific long noncoding RNAs in VSMC inflammation is poorly understood. Methods: Bulk RNA sequencing in diff…
View article: CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome
CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome Open
Microinjected transgenes, both large and small, are known to insert randomly into the mouse genome. Traditional methods of mapping a transgene are challenging, thus complicating breeding strategies and accurate interpretation of phenotypes…
View article: <i>INKILN</i>is a novel long noncoding RNA promoting vascular smooth muscle inflammation via scaffolding MKL1 and USP10
<i>INKILN</i>is a novel long noncoding RNA promoting vascular smooth muscle inflammation via scaffolding MKL1 and USP10 Open
Background Activation of vascular smooth muscle cells (VSMCs) inflammation is vital to initiate vascular disease. However, the role of human-specific long noncoding RNAs (lncRNAs) in VSMC inflammation is poorly understood. Methods Bulk RNA…
View article: PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII
PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII Open
View article: Prime Editing in Mice with an Engineered pegRNA
Prime Editing in Mice with an Engineered pegRNA Open
View article: Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells
Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells Open
View article: Mediterranean G6PD variant rats are protected from Angiotensin II-induced hypertension and kidney damage, but not from inflammation and arterial stiffness
Mediterranean G6PD variant rats are protected from Angiotensin II-induced hypertension and kidney damage, but not from inflammation and arterial stiffness Open
View article: An Inducible <i>Cre</i> Mouse with Preferential Activity in Vascular Smooth Muscle Evades a Previously Lethal Intestinal Phenotype
An Inducible <i>Cre</i> Mouse with Preferential Activity in Vascular Smooth Muscle Evades a Previously Lethal Intestinal Phenotype Open
All smooth muscle cell (SMC) restricted Cre mice recombine floxed alleles in vascular and visceral SMCs. We generated a new tamoxifen-inducible CreER T2 mouse, Itga8-CreER T2 , and compared its activity to the widely used Myh11-CreER T2 mo…
View article: Of mice and human-specific long noncoding RNAs
Of mice and human-specific long noncoding RNAs Open
View article: CRISPR-LRS for mapping transgenes in the mouse genome
CRISPR-LRS for mapping transgenes in the mouse genome Open
Microinjected transgenes, including bacterial artificial chromosomes (BACs), insert randomly in the mouse genome. Traditional methods of mapping a transgene are challenging, thus complicating breeding strategies and the accurate interpreta…
View article: Fate and State of Vascular Smooth Muscle Cells in Atherosclerosis
Fate and State of Vascular Smooth Muscle Cells in Atherosclerosis Open
Vascular smooth muscle cells (VSMCs) have long been associated with phenotypic modulation/plasticity or dedifferentiation. Innovative technologies in cell lineage tracing, single-cell RNA sequencing, and human genomics have been integrated…
View article: Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
View article: MKL1 cooperates with p38MAPK to promote vascular senescence, inflammation, and abdominal aortic aneurysm
MKL1 cooperates with p38MAPK to promote vascular senescence, inflammation, and abdominal aortic aneurysm Open
View article: Additional file 8 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 8 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 8. Supplementary Table S7. List of primers.
View article: Additional file 3 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 3 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 3. Supplementary Table S2. Dseq of bulk RNA-seq.
View article: Additional file 5 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 5 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 5. Supplementary Table S4. Pool 2 off-targets.
View article: Additional file 7 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 7 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 7. Supplementary Table S6. pegRNA off-targets and CArG boxes.
View article: Generating a CRISPR knockout mouse through a strong premature termination codon: a cautionary tale
Generating a CRISPR knockout mouse through a strong premature termination codon: a cautionary tale Open
View article: Additional file 6 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 6 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 6. Supplementary Table S5. sgRNA off-targets and CArG boxes.
View article: Additional file 2 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 2 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 2. Supplementary Table S1. CRISPR targets.
View article: Additional file 4 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Additional file 4 of Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression Open
Additional file 4. Supplementary Table S3. Pool 1 off-targets.