Josh Strable
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Unlocking grass leaf development: foundations for tunable cereal design Open
Summary The grass leaf plays a critical role in global food security, generating the carbon stores in cereal grains, which provide > 50% of global calories. As the global population grows, there is an urgent need to increase food productio…
Regulatory variation controlling architectural pleiotropy in maize Open
An early event in plant organogenesis is establishment of a boundary between the stem cell containing meristem and differentiating lateral organ. In maize ( Zea mays ), evidence suggests a common gene network functions at boundaries of dis…
Diverse roles of ethylene in maize growth and development, and its importance in shaping plant architecture Open
The gaseous plant hormone ethylene is a key developmental and growth regulator, and a pivotal endogenous response signal to abiotic and biotic interactions, including stress. Much of what is known about ethylene biosynthesis, perception, a…
A maize semi-dwarf mutant reveals a GRAS transcription factor involved in brassinosteroid signaling Open
Brassinosteroids (BR) and gibberellins (GA) regulate plant height and leaf angle in maize (Zea mays). Mutants with defects in BR or GA biosynthesis or signaling identify components of these pathways and enhance our knowledge about plant gr…
Transit amplifying cells balance growth and differentiation in above-ground meristems Open
In both animals and plants, stem cell niches balance between cell-renewal and the generation of progeny cells that differentiate into specialized tissues. The multipotent and highly proliferative transit amplifying cells (TACs) integrate s…
Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco Open
Allotopic expression is the term given for the deliberate relocation of gene function from an organellar genome to the nuclear genome. We hypothesized that the allotopic expression of an essential mitochondrial gene using a promoter that e…
Regulatory variation controlling architectural pleiotropy in maize Open
An early event in plant organogenesis is establishment of a boundary between the meristem and differentiating lateral organ. In maize ( Zea mays ), evidence suggests a common gene network functions at boundaries of distinct organs and cont…
Cytoplasmic Male Sterility and Abortive Seed Traits Generated through Mitochondrial Genome Editing Coupled with Allotopic Expression of <i>atp1</i> in Tobacco Open
Allotopic expression is the term given for the deliberate relocation of gene function from an organellar genome to the nuclear genome. We hypothesized that the allotopic expression of an essential mitochondrial gene using a promoter that e…
Interspecies transfer of <i>RAMOSA1</i> orthologs and promoter <i>cis</i> sequences impacts maize inflorescence architecture Open
Grass inflorescences support floral structures that each bear a single grain, where variation in branch architecture directly impacts yield. The maize (Zea mays) RAMOSA1 (ZmRA1) transcription factor acts as a key regulator of inflorescence…
Interspecies transfer of syntenic <i>RAMOSA1</i> orthologs and promoter <i>cis</i> sequences impacts maize inflorescence architecture Open
Grass inflorescences support floral structures that each bear a single grain, where variation in branch architecture directly impacts yield. The maize RAMOSA1 ( Zm RA1) transcription factor acts as a key regulator of inflorescence developm…
Distinct C <sub>4</sub> sub‐types and C <sub>3</sub> bundle sheath isolation in the Paniceae grasses Open
In C 4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct C 4 acid decarboxylases being used to release CO 2 in the vicinity of RuBisCO. For decades, these decarboxylases have been used …
An <i>in situ</i> sequencing approach maps <i>PLASTOCHRON1</i> at the boundary between indeterminate and determinate cells Open
The plant shoot apex houses the shoot apical meristem, a highly organized and active stem-cell tissue where molecular signaling in discrete cells determines when and where leaves are initiated. We optimized a spatial transcriptomics approa…
Distinct C4 sub-types and C3 bundle sheath isolation in the Paniceae grasses. Open
In C4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct C4 acid decarboxylases being used to release CO2 in the vicinity of RuBisCO. For decades, these decarboxylases have been used to …
View article: Plant stem-cell organization and differentiation at single-cell resolution
Plant stem-cell organization and differentiation at single-cell resolution Open
Significance Plants possess the remarkable ability to grow and produce new organs throughout their lifespan, owing to the activities of persistent populations of pluripotent stem cells within their meristematic tips. Here we isolated indiv…
Gains in Grain Yield: A Pair of Spikelets Makes All the Difference, Even When One Is Sterile Open
Mother Nature has a way of keeping seemingly useless structures around millions of years. Such structures are likely to have a use that is not obvious, although they could also be remnants of the evolutionary past without extant function o…
View article: Plant stem cell organization and differentiation at single-cell resolution
Plant stem cell organization and differentiation at single-cell resolution Open
Plants maintain populations of pluripotent stem cells in shoot apical meristems (SAMs), which continuously produce new aboveground organs. We used single-cell RNA sequencing to achieve an unbiased characterization of the transcriptional la…
Sugars Inform the Circadian Clock How to Shape Rice Shoots via the Strigolactone Pathway Open
Circadian clocks act as universal timekeepers to harmonize internal processes with external day/night rhythms. Genetic feedback loops gear these inner clocks by approximating time in response to dawn/dusk cycles. In plants, development, gr…
Maize Introgression Library Provides Evidence for the Involvement of <i>liguleless1</i> in Resistance to Northern Leaf Blight Open
Plant disease resistance is largely governed by complex genetic architecture. In maize, few disease resistance loci have been characterized. Near-isogenic lines are a powerful genetic tool to dissect quantitative trait loci. We analyzed an…
The FUSED LEAVES1‐ <i>ADHERENT1</i> regulatory module is required for maize cuticle development and organ separation Open
Summary All aerial epidermal cells in land plants are covered by the cuticle, an extracellular hydrophobic layer that provides protection against abiotic and biotic stresses and prevents organ fusion during development. Genetic and morphol…
Peptide-Receptor Signaling Pumps the Brakes on Auxin Biosynthesis and Ethylene Signaling to Harmonize Root Growth and Nodulation Open
Nitrogen (N) is the most abundant element in Earth’s atmosphere. However, plants must capture this essential element from soil through their roots. To do this, legume roots forge symbioses with rhizobia to initiate nodule development. Root…
Activate, Breakdown, Branch Out: CUC2/3-DA1-UBP15 Controls Axillary Meristem Initiation Open
Shoot architecture is a key ecological and agricultural trait that impacts biomass, the potential to harvest light, planting density, and the reproductive success of a plant. Branching is a prominent feature of plant shoot architecture. A …
Cytokinin Signaling Patterns Maize Leaves, Otherwise Things Get Hairy and Frayed Open
Variation in leaf morphology across the angiosperms is impressive and abounds. Leaves are the primary photosynthetic organ for most angiosperms, acting as the primary sites of light capture and gas exchange. Typical of grass leaves, maize …
Keeping the Ethylene Response Fluid: GDSL Lipase MHZ11 Modulates Sterol Levels and Ethylene Signaling in Rice Roots Open
Nature’s simplest alkene, ethylene, is a gaseous phytohormone produced in planta at certain developmental stages and in response to abiotic and biotic stress. Three decades of elegant genetic, molecular, and biochemical studies conducted i…
The FUSED LEAVES1/ <i>ADHERENT1</i> Regulatory Module Is Required For Maize Cuticle Development And Organ Separation Open
In land plants all aerial epidermal cells are covered by the cuticle, an extracellular hydrophobic layer. The cuticle represents a primary barrier between cells and the external environment, provides protection against abiotic and biotic s…
Maize nested introgression library provides evidence for the involvement of <i>liguleless1</i> in resistance to northern leaf blight Open
Plant disease resistance is largely governed by complex genetic architecture. In maize, few disease resistance loci have been characterized. Near-isogenic lines (NILs) are a powerful genetic tool to dissect quantitative trait loci (QTL). W…
Maize <i>YABBY</i> genes <i>drooping leaf1</i> and <i>drooping leaf2</i> regulate floret development and floral meristem determinacy Open
Floral morphology is shaped by factors that modulate floral meristem activity and size, and the identity, number and arrangement of the lateral organs they form. We report the maize CRABS CLAW co-orthologs drooping leaf1 (drl1) and drl2 ar…
Maize <i>YABBY drooping leaf</i> genes regulate floret development and floral meristem determinacy Open
Floret units in cereals produce grain, directly impacting yield. Here we report mutations in the maize CRABS CLAW ( CRC ) co-orthologs drooping leaf1 ( drl1 ) and drl2 alter the development of ear and tassel florets. Pistillate florets of …