Joshua T. Morgan
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View article: Bicontinuous Interconnected Porous Biomaterials for Tissue Engineering and Regeneration
Bicontinuous Interconnected Porous Biomaterials for Tissue Engineering and Regeneration Open
Biomaterials mimicking natural extracellular matrix are necessary to create an optimal microenvironment for cell adhesion, migration, proliferation, and differentiation. These scaffolds must possess bicontinuous interconnected porosity to …
View article: Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells
Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells Open
Mitochondrial DNA (mtDNA) damage in trabecular meshwork (TM) cells occurs in open-angle glaucoma (OAG). However, current in vitro models for OAG-like changes in TM cells do not explicitly incorporate mtDNA damage. This work validated two m…
View article: Expressing intrinsically-disordered tardigrade proteins has positive effects on acute but not chronic stress tolerance in Saccharomyces cerevisiae
Expressing intrinsically-disordered tardigrade proteins has positive effects on acute but not chronic stress tolerance in Saccharomyces cerevisiae Open
The production of high value and commodity chemicals, biopharmaceuticals and biofuels using Saccharomyces cerevisiae is hindered by various stress factors that affect yield and efficiency. Tardigrades, known for their remarkable stress tol…
View article: Meibomian gland lipid alterations and ocular surface sequela in Awat2 knockout murine model of meibomian gland dysfunction and evaporative dry eye disease
Meibomian gland lipid alterations and ocular surface sequela in Awat2 knockout murine model of meibomian gland dysfunction and evaporative dry eye disease Open
Awat2 KO mice recapitulate many of features of human MGD and EDED, representing a model to test novel therapeutics.
View article: Dexamethasone Impairs ATP Production and Mitochondrial Performance in Human Trabecular Meshwork Cells
Dexamethasone Impairs ATP Production and Mitochondrial Performance in Human Trabecular Meshwork Cells Open
Mitochondrial damage occurs in human trabecular meshwork (HTM) cells as a result of normal aging and in open angle glaucoma. Using an HTM cell model, we quantified mitochondrial function and ATP generation rates after dexamethasone (Dex) a…
View article: The tardigrade-derived mitochondrial abundant heat soluble protein improves adipose-derived stem cell survival against representative stressors
The tardigrade-derived mitochondrial abundant heat soluble protein improves adipose-derived stem cell survival against representative stressors Open
View article: Preliminary evaluation of safety and migration of immune activated mesenchymal stromal cells administered by subconjunctival injection for equine recurrent uveitis
Preliminary evaluation of safety and migration of immune activated mesenchymal stromal cells administered by subconjunctival injection for equine recurrent uveitis Open
Introduction Equine recurrent uveitis (ERU), an immune mediated disease characterized by repeated episodes of intra-ocular inflammation, affects 25% of horses in the USA and is the most common cause of glaucoma, cataracts, and blindness. M…
View article: In vitro formation and extended culture of highly metabolically active and contractile tissues
In vitro formation and extended culture of highly metabolically active and contractile tissues Open
3D cell culture models have gained popularity in recent years as an alternative to animal and 2D cell culture models for pharmaceutical testing and disease modeling. Polydimethylsiloxane (PDMS) is a cost-effective and accessible molding ma…
View article: Surrogate modeling for CO2 capture by chemical absorption based on data from rigorous model optimization
Surrogate modeling for CO2 capture by chemical absorption based on data from rigorous model optimization Open
Modular CO2 capture plants can help reducing the cost of deploying capture systems across the globe. However, the CO2 variability and model uncertainty represent operational challenges to capture CO2 from different sources. This work propo…
View article: <i>In vitro</i>formation and extended culture of highly metabolically active and contractile tissues
<i>In vitro</i>formation and extended culture of highly metabolically active and contractile tissues Open
3D cell culture models have gained popularity in recent years as an alternative to animal and 2D cell culture models for pharmaceutical testing and disease modeling. Polydimethylsiloxane (PDMS) is a cost-effective and accessible molding ma…
View article: Lipid-Sensing Receptor FFAR4 Modulates Pulmonary Epithelial Homeostasis following Immunogenic Exposures Independently of the FFAR4 Ligand Docosahexaenoic Acid (DHA)
Lipid-Sensing Receptor FFAR4 Modulates Pulmonary Epithelial Homeostasis following Immunogenic Exposures Independently of the FFAR4 Ligand Docosahexaenoic Acid (DHA) Open
The role of pulmonary free fatty acid receptor 4 (FFAR4) is not fully elucidated and we aimed to clarify the impact of FFAR4 on the pulmonary immune response and return to homeostasis. We employed a known high-risk human pulmonary immunoge…
View article: Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies
Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies Open
Photoaging is an important extrinsic aging factor leading to altered skin morphology and reduced function. Prior work has revealed a connection between photoaging and loss of subcutaneous fat. Currently, primary models for studying this ar…
View article: Organotypic cultures as aging associated disease models
Organotypic cultures as aging associated disease models Open
Aging remains a primary risk factor for a host of diseases, including leading causes of death. Aging and associated diseases are inherently multifactorial, with numerous contributing factors and phenotypes at the molecular, cellular, tissu…
View article: Automated Epidermal Thickness Quantification of <i>in Vitro</i> Human Skin Equivalents Using Optical Coherence Tomography
Automated Epidermal Thickness Quantification of <i>in Vitro</i> Human Skin Equivalents Using Optical Coherence Tomography Open
Human skin equivalents (HSEs) are in vitro models of human skin. They are used to study skin development, diseases, wound healing and toxicity. The gold standard of analysis is histological sectioning, which both limits three-dimens…
View article: Priming stroma with a vitamin D analog to optimize viroimmunotherapy for pancreatic cancer
Priming stroma with a vitamin D analog to optimize viroimmunotherapy for pancreatic cancer Open
View article: Efficient facemask decontamination via forced ozone convection
Efficient facemask decontamination via forced ozone convection Open
View article: Generation of Self-assembled Vascularized Human Skin Equivalents
Generation of Self-assembled Vascularized Human Skin Equivalents Open
Human skin equivalents (HSEs) are tissue engineered constructs that model epidermal and dermal components of human skin. These models have been used to study skin development, wound healing, and grafting techniques. Many HSEs continue to l…
View article: Determining the dielectric constant of injection-molded polymer-matrix nanocomposites filled with barium titanate
Determining the dielectric constant of injection-molded polymer-matrix nanocomposites filled with barium titanate Open
View article: Efficient Facemask Sterilization via Forced Ozone Convection
Efficient Facemask Sterilization via Forced Ozone Convection Open
During the beginning of 2020, the Covid-19 pandemic took the world by surprise, rapidly spreading undetected between and within many countries and wreaking havoc on the global economy both through death tolls and lockdowns. Healthcare prof…
View article: Ubiquitous Nuclear Threat Detection Final Report, Harvey Mudd College Engineering Clinic
Ubiquitous Nuclear Threat Detection Final Report, Harvey Mudd College Engineering Clinic Open
The Lawrence Livermore National Labs clinic team was asked to design a repeatable, portable procedure that would incentivize participants to carry detectors that form a radiation sensor network along repeated paths, to precisely known loca…
View article: Generation and morphological quantification of large scale, three-dimensional, self-assembled vascular networks
Generation and morphological quantification of large scale, three-dimensional, self-assembled vascular networks Open
One of the largest issues facing the field of tissue engineering is scaling due to tissue necrosis as a result of a lack of vascularization. We have developed an accessible method for generating large scale vascular networks of arbitrary g…
View article: Regional Variation in Androgen Receptor Expression and Biomechanical Properties May Contribute to Cryptorchidism Susceptibility in the LE/orl Rat
Regional Variation in Androgen Receptor Expression and Biomechanical Properties May Contribute to Cryptorchidism Susceptibility in the LE/orl Rat Open
Background: The process of testicular descent requires androgen and insulin-like 3, hormones secreted by fetal Leydig cells. Knowledge concerning distinct and common functions of these hormones in regulating development of the fetal…
View article: The Mechanosensitive Ion Channel TRPV4 is a Regulator of Lung Development and Pulmonary Vasculature Stabilization
The Mechanosensitive Ion Channel TRPV4 is a Regulator of Lung Development and Pulmonary Vasculature Stabilization Open
View article: Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide
Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide Open
Resistance to chemotherapy substantially hinders successful glioblastoma (GBM) treatment, contributing to an almost 100% mortality rate. Resistance to the frontline chemotherapy, temozolomide (TMZ), arises from numerous signaling pathways …
View article: Latrunculin B and substratum stiffness regulate corneal fibroblast to myofibroblast transformation
Latrunculin B and substratum stiffness regulate corneal fibroblast to myofibroblast transformation Open
View article: Cyclic Nucleotide-Directed Protein Kinases in Cardiovascular Inflammation and Growth
Cyclic Nucleotide-Directed Protein Kinases in Cardiovascular Inflammation and Growth Open
Cardiovascular disease (CVD), including myocardial infarction (MI) and peripheral or coronary artery disease (PAD, CAD), remains the number one killer of individuals in the United States and worldwide, accounting for nearly 18 million (>30…
View article: Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model
Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model Open
Laser-induced experimental glaucoma (ExGl) in non-human primates (NHPs) is a common animal model for ocular drug development. While many features of human hypertensive glaucoma are replicated in this model, structural and functional change…
View article: Dynamic compensation occurs in the aqueous outflow pathway in non-human primates with experimental glaucoma
Dynamic compensation occurs in the aqueous outflow pathway in non-human primates with experimental glaucoma Open
View article: Dexamethasone Stiffens Trabecular Meshwork, Trabecular Meshwork Cells, and Matrix
Dexamethasone Stiffens Trabecular Meshwork, Trabecular Meshwork Cells, and Matrix Open
This integrated approach clearly demonstrates that DEX treatment increases TM cell stiffness concurrent with elevated αSMA expression and activation of the mitogen-activated protein kinase (MAPK) pathway, stiffens the ECM in vitro along wi…
View article: The intrinsic stiffness of human trabecular meshwork cells increases with senescence
The intrinsic stiffness of human trabecular meshwork cells increases with senescence Open
Dysfunction of the human trabecular meshwork (HTM) plays a central role in the age-associated disease glaucoma, a leading cause of irreversible blindness. The etiology remains poorly understood but cellular senescence, increased stiffness …