Joy Arakaki
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View article: Integrated intracellular organization and its variations in human iPS cells
Integrated intracellular organization and its variations in human iPS cells Open
Understanding how a subset of expressed genes dictates cellular phenotype is a considerable challenge owing to the large numbers of molecules involved, their combinatorics and the plethora of cellular behaviours that they determine 1,2 . H…
View article: A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes
A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes Open
We performed a comprehensive analysis of the transcriptional changes within and across cell populations during human induced pluripotent stem cell (hiPSC) differentiation to cardiomyocytes. Using the single cell RNA-seq combinatorial barco…
View article: Automated hiPSC culture and sample preparation for 3D live cell microscopy
Automated hiPSC culture and sample preparation for 3D live cell microscopy Open
Our goal is to identify and understand cellular behaviors using 3D live imaging of cell organization. To do this, we image human inducible pluripotent stem cell (hiPSC) lines expressing fluorescently tagged protein representing specific ce…
View article: Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Open
A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly interspaced …
View article: Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells
Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells Open
We describe a multi-step CRISPR/Cas9 gene editing method to create endogenously tagged GFP-fusions of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs), allowing visualization of proteins that are only express…
View article: Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization
Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization Open
We present a CRISPR/Cas9 genome editing strategy to systematically tag endogenous proteins with fluorescent tags in human inducible pluripotent stem cells. To date we have generated multiple human iPSC lines with GFP tags for 10 proteins r…