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Efficient gene targeting in soybean using <i>Ochrobactrum haywardense</i>-mediated delivery of a marker-free donor template Open
Gene targeting (GT) for precise gene insertion or swap into pre-defined genomic location has been a bottleneck for expedited soybean precision breeding. We report a robust selectable marker-free GT system in soybean, one of the most econom…
Development of an efficient marker‐free soybean transformation method using the novel bacterium <i>Ochrobactrum haywardense</i> H1 Open
Summary We have discovered a novel bacterium, Ochrobactrum haywardense H1 ( Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium ‐derived vir and T‐DNA‐mediated transformation. Oh H1 is a…
View article: A giant NLR gene confers broad-spectrum resistance to Phytophthora sojae in soybean
A giant NLR gene confers broad-spectrum resistance to Phytophthora sojae in soybean Open
Phytophthora root and stem rot caused by P. sojae is a destructive soybean soil-borne disease found worldwide. Discovery of genes conferring broad-spectrum resistance to the pathogen is a need to prevent the outbreak of the disease. Here, …
Efficient, selectable marker free gene targeting in soybean using novel <i>Ochrobactrum haywardense</i> -mediated delivery Open
We report robust selectable marker-free gene targeting (GT) system in soybean, one of the most economically important crops. A novel efficient Ochrobactrum haywardense -mediated embryonic axis transformation method was used for the deliver…
A giant chimeric NLR gene confers extreme broad-spectrum resistance to a plant pathogen Open
Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae is the most destructive soybean soil-borne disease worldwide. Discovery of genes conferring broad-spectrum resistance to the pathogen is an urgent need to prevent the outbr…
Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean Open
Development of transgenic cell lines or organisms for industrial, agricultural, or medicinal applications involves inserting DNA into the target genome in a way that achieves efficacious transgene expression without a deleterious impact on…