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View article: Co-isolation of genetically distinct Burkholderia pseudomallei strains from a single patient in North Queensland
Co-isolation of genetically distinct Burkholderia pseudomallei strains from a single patient in North Queensland Open
While investigating colony morphology variation within clinical isolates of Burkholderia pseudomallei ( Bp) isolated from a single clinical infection, we identified two distinct strains (based on their multi-locus sequence type) from Bp TS…
View article: Co-isolation of genetically distinct <i>Burkholderia pseudomallei</i> strains from a single patient in North Queensland
Co-isolation of genetically distinct <i>Burkholderia pseudomallei</i> strains from a single patient in North Queensland Open
While investigating colony morphology variation (CMV) within clinical isolates of Burkholderia pseudomallei ( Bp ) isolated from a single infection, we identified two distinct strains (based on their multi-locus sequence type) from Bp TSV2…
View article: Profiling of <i>Burkholderia pseudomallei</i> variants derived from Queensland’s clinical isolates
Profiling of <i>Burkholderia pseudomallei</i> variants derived from Queensland’s clinical isolates Open
Burkholderia pseudomallei ( Bp ), an environmental bacterium and opportunistic pathogen endemic to tropical regions, is highly adaptive and thrives in diverse environments, from soil to human hosts. Bacterial adaptation is critical for sur…
View article: Detecting RNA Viruses in Cattle: Effects of Sequencing Depth and Sequence References in Metatranscriptomics
Detecting RNA Viruses in Cattle: Effects of Sequencing Depth and Sequence References in Metatranscriptomics Open
In this study, the impact of sequencing depth and reference genome selection is shown to influence the detection of five bovine respiratory RNA viruses in metatranscriptomes from clinical cattle samples. Metatranscriptomes were generated f…
View article: An immunoregulatory amphipathic peptide derived from <scp> <i>Fasciola hepatica</i> </scp> helminth defense molecule ( <scp>FhHDM</scp> ‐1. <scp>C2</scp> ) exhibits potent biotherapeutic activity in a murine model of multiple sclerosis
An immunoregulatory amphipathic peptide derived from <span> <i>Fasciola hepatica</i> </span> helminth defense molecule ( <span>FhHDM</span> ‐1. <span>C2</span> ) exhibits potent biotherapeutic activity in a murine model of multiple sclerosis Open
The helminth defense molecules (HDM) are a family of immune regulatory peptides exclusively expressed by trematode worms. We have previously demonstrated that in vivo FhHDM‐1, the archetypal member of the HDMs, regulated macrophage respons…
View article: Single cell RNA-seq reveals that the Vibrio cholerae MakA toxin is required for killing of Tetrahymena pyriformis and for survival in protozoan expelled food vacuoles
Single cell RNA-seq reveals that the Vibrio cholerae MakA toxin is required for killing of Tetrahymena pyriformis and for survival in protozoan expelled food vacuoles Open
Vibrio cholerae uses multiple strategies to resist predation by heterotrophic protozoa. For example, V. cholerae releases toxic compounds such as ammonium and pyomelanin, that can kill protists such as Tetrahymena pyriformis. V. cholerae a…
View article: Increased iron utilization and oxidative stress tolerance in a <i>Vibrio cholerae flrA</i> mutant confers resistance to amoeba predation
Increased iron utilization and oxidative stress tolerance in a <i>Vibrio cholerae flrA</i> mutant confers resistance to amoeba predation Open
The flagellar transcriptional regulator flrA initiates the regulatory cascade of flagellum synthesis in Vibrio cholerae . Previously, we observed an increase in sub-populations of V. cholerae carrying mutations in the flrA gene during long…
View article: Macrophage migration inhibitory factor promotes glucocorticoid resistance of neutrophilic inflammation in a murine model of severe asthma
Macrophage migration inhibitory factor promotes glucocorticoid resistance of neutrophilic inflammation in a murine model of severe asthma Open
Background Severe neutrophilic asthma is resistant to treatment with glucocorticoids. The immunomodulatory protein macrophage migration inhibitory factor (MIF) promotes neutrophil recruitment to the lung and antagonises responses to glucoc…
View article: Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Strains Recovered from Chilean Hospitals Reveals Lineages Specific to South America and Multiple Routes for Acquisition of Antibiotic Resistance Genes
Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Strains Recovered from Chilean Hospitals Reveals Lineages Specific to South America and Multiple Routes for Acquisition of Antibiotic Resistance Genes Open
Treating infections caused by carbapenem-resistant A. baumannii (CR Ab ) has become a global challenge given that CR Ab strains are also often resistant to a wide range of antibiotics. Analysis of whole-genome sequence data is now a standa…
View article: Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction
Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction Open
We developed a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 14 times more libraries for high-throughput Illumina sequencing to be generated for the same cost. We call this new method Hack…
View article: Two carbapenem-resistant ST1:ST231:KL1:OCL1<i>Acinetobacter baumannii</i>strains recovered in Tehran, Iran, carry AbaR31 in the chromosome and AbaR4 and Tn<i>aphA6</i>in a RepAci6 plasmid
Two carbapenem-resistant ST1:ST231:KL1:OCL1<i>Acinetobacter baumannii</i>strains recovered in Tehran, Iran, carry AbaR31 in the chromosome and AbaR4 and Tn<i>aphA6</i>in a RepAci6 plasmid Open
Objectives To analyse the context of genes conferring antibiotic resistance in two carbapenem-resistant Acinetobacter baumannii isolates recovered in Tehran, Iran. Methods The antibiotic resistance phenotype for 28 antibiotics was determin…
View article: Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage Open
Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a…
View article: Complete Genome Sequence of Stenotrophomonas maltophilia Strain CF13, Recovered from Sputum from an Australian Cystic Fibrosis Patient
Complete Genome Sequence of Stenotrophomonas maltophilia Strain CF13, Recovered from Sputum from an Australian Cystic Fibrosis Patient Open
Stenotrophomonas maltophilia isolate CF13 is a multidrug-resistant isolate that was recovered in Sydney, Australia, in 2011, from a sputum sample from an individual with cystic fibrosis. The genome sequence of CF13 was completed using long…
View article: Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs)
Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs) Open
These results offer new insights into the structure and function of a well-known immunomodulatory molecule, Sm16, and places it within a wider family of trematode-specific small molecule HDM immune-modulators with immuno-biotherapeutic pos…
View article: A protocol for massively parallel diagnosis and genome sequencing of SARS-CoV-2 v1
A protocol for massively parallel diagnosis and genome sequencing of SARS-CoV-2 v1 Open
Managing the current COVID-19 pandemic requires diagnostic testing at an unprecedented scale. However, the crisis has revealed severe deficiencies in our capacity to perform such testing. Here we build on the work of others (1-3) to develo…
View article: Additional file 1: of Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages
Additional file 1: of Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages Open
Tables S1 and S2. Gene expression in murine macrophages treated with LPS. Microarray analysis of gene expression in macrophages treated with LPS for 6Â h (Table S1.) and 24Â h (Table S2.) (XLSX 1623 kb)
View article: A parasite-derived 68-mer peptide ameliorates autoimmune disease in murine models of Type 1 diabetes and multiple sclerosis
A parasite-derived 68-mer peptide ameliorates autoimmune disease in murine models of Type 1 diabetes and multiple sclerosis Open
Helminth parasites secrete molecules that potently modulate the immune responses of their hosts and, therefore, have potential for the treatment of immune-mediated human diseases. FhHDM-1, a 68-mer peptide secreted by the helminth parasite…
View article: The immune modulatory peptide FhHDM‐1 secreted by the helminth Fasciola hepatica prevents NLRP3 inflammasome activation by inhibiting endolysosomal acidification in macrophages
The immune modulatory peptide FhHDM‐1 secreted by the helminth Fasciola hepatica prevents NLRP3 inflammasome activation by inhibiting endolysosomal acidification in macrophages Open
The NLRP3 inflammasome is a multimeric protein complex that controls the production of IL‐1β, a cytokine that influences the development of both innate and adaptive immune responses. Helminth parasites secrete molecules that interact with …