Explanipedia

3D-organization and spatial localization of chromatin and epigenetic marks linked to nucleolar activity in porcine oocytes Open

Merle Friederike Fenner, Michal Benc, Alexandra Bartková, Maria Pihl, Martine Chebrout , et al. · 2025

Previous murine studies have established that large-scale chromatin modifications upon completion of oocyte growth are associated with nucleolar transcriptional silencing. These modifications seem essential both for completion of the oocyt…

Mouse Nucleolus-Like Body Supports Transcription and Enhances Processing of rRna in Porcine Enucleolated Oocytes Open

Martin Morovič, František Strejček, Michal Benc, Alexandra Bartková, Sara Stachovicova , et al. · 2025

Fully-grown mammalian oocytes contain morphologically evident but transcriptionally inactive nucleoli called “nucleolus-like bodies” (NLBs). These nuclear structures are essential for early embryonic development. Removing oocyte NLBs (enuc…

Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro Open

Alexandra Bartková, Lucie Němcová, František Strejček, Ahmed Gad, Veronika Kinterová , et al. · 2024

Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium) Open

Alexandra Bartková, Lucie Němcová, Veronika Kinterová, Dominika Radová, František Strejček , et al. · 2024

Background Oocytes of large animal species isolated from small ovarian follicles (< 2 mm) are less competent to support early embryonic development after in vitro maturation and fertilization than their counterparts isolated from medium-si…

Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study Open

Monika Dovenska, Branko Atanasov, Michal Benc, Martin Nikolovski, L. Pendovski , et al. · 2023

Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. The aim of the current study was to assess the relationship between…

Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality Open

Ahmed Gad, Matej Murín, Alexandra Bartková, Veronika Kinterová, Kateřina Marcollová , et al. · 2022

Additional file 1 of Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality Open

Ahmed Gad, Matej Murín, Alexandra Bartková, Veronika Kinterová, Kateřina Marcollová , et al. · 2022

Additional file 1: Table S1. Summary of sequence reads mapped to the porcine reference genome and annotated against porcine miRNAs listed in the mirBase database. Table S2. A complete list of all expressed miRNAs in the high (HQ) and low (…

The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium Open

Radek Procházka, Alexandra Bartková, Lucie Němcová, Matej Murín, Ahmed Gad , et al. · 2021

The developmental potential of porcine oocytes cultured in vitro was remarkably enhanced in a medium containing FGF2, LIF and IGF1 (FLI) when compared to a medium supplemented with gonadotropins and EGF (control). We analyzed the molecular…

Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy Open

Michal Benc, František Strejček, Martin Morovič, Alexandra Bartková, Matej Murín , et al. · 2021

The nucleolus is an important nucleus sub-organelle found in almost all eukaryotic cells. On the one hand, it is known as a differentiated active site of ribosome biogenesis in somatic cells, but on the other hand, in fully grown oocytes, …

Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos Open

Ahmed Gad, Matej Murín, Lucie Němcová, Alexandra Bartková, Jozef Laurinčík , et al. · 2020

Oocyte developmental competence is regulated by various mechanisms and molecules including microRNAs (miRNAs). However, the functions of many of these miRNAs in oocyte and embryo development are still unclear. In this study, we managed to …

Plasma extracellular vesicle miRNAs as potential biomarkers of superstimulatory response in cattle Open

Ahmed Gad, José María Sánchez, John A. Browne, Lucie Němcová, Jozef Laurinčík , et al. · 2020

The ability to predict superstimulatory response would be a beneficial tool in assisted reproduction. Using small RNAseq technology, we profiled extracellular vesicle microRNA (EV-miRNA) abundance in the blood plasma of heifers exhibiting …

Inhibition of miR-152 During in Vitro Maturation Enhances the Developmental Potential of Porcine Embryos Open

Ahmed Gad, Matej Murín, Lucie Němcová, Alexandra Bartková, Jozef Laurinčík , et al. · 2020

Background Oocyte developmental competence is regulated by various mechanisms and molecules including microRNAs (miRNAs). However, the functions of many of these miRNAs in oocyte and embryo development are still unclear. In this study, we …

Gene expression during pre- and peri-implantation embryonic development in pigs Open

P. Maddox‐Hyttel, András Dinnyés, Jozef Laurinčík, D. Rath, H. Niemann , et al. · 2020

Embryo technological procedures such as in vitro production and cloning by nuclear transfer are not as advanced in pigs as in cattle and cannot yet be applied under field conditions. The present paper focuses on genome activation in in viv…

Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells Open

Samuel Gebremedhn, Ahmed Gad, Hoda Samir Aglan, Jozef Laurinčík, Radek Procházka , et al. · 2020

Elevated summer temperature is reported to be the leading cause of stress in dairy and beef cows, which negatively affects various reproductive functions. Follicular cells respond to heat stress (HS) by activating the expression of heat sh…

Table of Contents, Volume 87, Issue 1, January 2020 Open

Willie J. Swanson, G. González Rey, David M. Findlay, Vishakha Mahajan, Cindy Farquhar , et al. · 2020

Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro Open

Alexandra Bartková, Martin Morovič, František Strejček, Matej Murín, Michal Benc , et al. · 2020

Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological …

Morphological assessment of preimplantation embryo quality in cattle Open

P. Maddox‐Hyttel, JO Gjørret, G. Vajta, N.I. Alexopoulos, Ian Lewis , et al. · 2019

The extensive use of embryo technologies has emphasized the need for assessing embryo quality by morphological techniques, such as transmission electron microscopy, immunocytochemistry for confocal laser scanning microscopy and fluorescenc…

Enucleolation and nucleolus transfer in mammalian oocytes and zygotes Open

Michal Benc, Josef Fulka, František Strejček, Martin Morovič, Matej Murín , et al. · 2019

The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easi…

Oocytes, embryos and pluripotent stem cells from a biomedical perspective Open

P. Hyttel, Laís Vicari de Figueirêdo Pessôa, Jan Bojsen‐Møller Secher, Katarina Stoklund Dittlau, Kristine Freude , et al. · 2019

The veterinary and animal science professions are rapidly developing and their inherent and historical connection to agriculture is challenged by more biomedical and medical directions of research. While some consider this development as a…

The Oocyte’s Nucleolus Precursor Body: The Globe for Life Open

Michal Benc, L. Pendovski, Matej Murín, František Strejček, Martin Morovič , et al. · 2018

The nucleolus is the cell organelle responsible for ribosome synthesis and, hence, for protein synthesis. In the mammalian oocyte, the nucleolus compacts into a dense sphere with no ribosome synthesis well in advance of ovulation. It seems…

Nucleologenesis and Nucleolotransfer in Mammalian Oocytes: A Review Open

Michal Benc, František Strejček, Matej Murín, Martin Morovič, Stanislava Martínková , et al. · 2017

An effort to improve development potential of early embryos is one of the main goals of biotechnology in the area of reproductive biology with application in veterinary or human medicine. Recent observations of the function of nucleolus or…

The Influence of Interspecies Somatic Cell Nuclear Transfer on Epigenetic Enzymes Transcription in Early Embryos Open

Martin Morovič, Matej Murín, František Strejček, Michal Benc, Dušan Paál , et al. · 2016

One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is a…

THE INFLUENCE OF THE OOPLASM ON DNMT1 AND DNMT3A GENE EXPRESSION Open

Martin Morovič, František Strejček, Miroslav Perniš, Oľga Østrup, Andrea Lucas‐Hahn , et al. · 2015

Developmental incompetence of embryos constructed by somatic cell nuclear transfer is caused by insuffi cient reprogramming of the transferred nucleus to a state equivalent to that of an early embryonic nucleus. Preceding studies have show…

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