Juan Sánchez-Weatherby
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View article: Hundred-Picolitre Droplet on Demand Tape Drive and XES Prototypes for Time-Resolved Serial Crystallography at VMXi, Diamond Light Source
Hundred-Picolitre Droplet on Demand Tape Drive and XES Prototypes for Time-Resolved Serial Crystallography at VMXi, Diamond Light Source Open
Time resolved X-ray crystallography is experiencing a resurgence in part because of serial methods that readily allow scientists to create stop-motion movies of macromolecular function of photoactivation, enzyme catalysed reactions, and si…
View article: The Single-Particle, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European X-ray Free-Electron Laser: Interaction Region Downstream at atmospheric pressure (IRD)
The Single-Particle, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European X-ray Free-Electron Laser: Interaction Region Downstream at atmospheric pressure (IRD) Open
The Single-Particle, Clusters and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) scientific instrument at the European X-Ray Free-Electron Laser (EuXFEL) became operational with user experiments in September 2017. The unique…
View article: Does crossing the pond affect crystal quality?
Does crossing the pond affect crystal quality? Open
Room-temperature (RT) X-ray diffraction experiments enable us to investigate protein dynamics, efficiently probe fragment binding, and perform time-resolved crystallography experiments. The Versatile Macromolecular Crystallography in-situ …
View article: Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations
Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations Open
Multi-crystal processing of X-ray diffraction data has become highly automated to keep pace with the current high-throughput capabilities afforded by beamlines. A significant challenge, however, is the automated clustering of such data bas…
View article: Effects of mineralization on the hierarchical organization of collagen—a synchrotron X-ray scattering and polarized second harmonic generation study
Effects of mineralization on the hierarchical organization of collagen—a synchrotron X-ray scattering and polarized second harmonic generation study Open
The process of mineralization fundamentally alters collagenous tissue biomechanics. While the structure and organization of mineral particles have been widely studied, the impact of mineralization on collagen matrix structure, particularly…
View article: Crystallization and <em>In Situ</em> Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source
Crystallization and <em>In Situ</em> Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source Open
Protocols for robotic protein crystallization using the Crystallization Facility at Harwell and in situ room temperature data collection from crystallization plates at Diamond Light Source beamline VMXi are described. This approach enables…
View article: Efficient <i>in situ</i> screening of and data collection from microcrystals in crystallization plates
Efficient <i>in situ</i> screening of and data collection from microcrystals in crystallization plates Open
A considerable bottleneck in serial crystallography at XFEL and synchrotron sources is the efficient production of large quantities of homogenous, well diffracting microcrystals. Efficient high-throughput screening of batch-grown microcrys…
View article: Protein-to-structure pipeline for ambient-temperature <i>in situ</i> crystallography at VMXi
Protein-to-structure pipeline for ambient-temperature <i>in situ</i> crystallography at VMXi Open
The utility of X-ray crystal structures determined under ambient-temperature conditions is becoming increasingly recognized. Such experiments can allow protein dynamics to be characterized and are particularly well suited to challenging pr…
View article: <i>xia</i>2.<i>multiplex</i>: a multi-crystal data-analysis pipeline
<i>xia</i>2.<i>multiplex</i>: a multi-crystal data-analysis pipeline Open
In macromolecular crystallography, radiation damage limits the amount of data that can be collected from a single crystal. It is often necessary to merge data sets from multiple crystals; for example, small-wedge data collections from micr…
View article: Tropocollagen springs allow collagen fibrils to stretch elastically
Tropocollagen springs allow collagen fibrils to stretch elastically Open
View article: xia2.multiplex: a multi-crystal data analysis pipeline
xia2.multiplex: a multi-crystal data analysis pipeline Open
In macromolecular crystallography radiation damage limits the amount of data that can be collected from a single crystal. It is often necessary to merge data sets from multiple crystals, for example small-wedge data collections on micro-cr…
View article: Multicrystal Datasets of Thermolysin collected using a multilayer monochromator
Multicrystal Datasets of Thermolysin collected using a multilayer monochromator Open
These are a series of datasets which have been collected on the VMXi beamline at the Diamond Light Source, UK. They were collected in-situ at room temperature using a Dectris 2X 4M detector. The datasets were collected in unattend…
View article: Multicrystal Datasets of Thermolysin collected using a multilayer monochromator
Multicrystal Datasets of Thermolysin collected using a multilayer monochromator Open
These are a series of datasets which have been collected on the VMXi beamline at the Diamond Light Source, UK. They were collected in-situ at room temperature using a Dectris 2X 4M detector. The datasets were collected in unattend…
View article: Multicrystal data of proteinase K collected on the VMXi beamline at the Diamond Light Source, UK
Multicrystal data of proteinase K collected on the VMXi beamline at the Diamond Light Source, UK Open
These are a series of datasets which have been collected on the VMXi beamline at the Diamond Light Source, UK. They were collected in-situ at room temperature using a Dectris 2X 4M detector. The datasets were collected in unattend…
View article: Multicrystal data of proteinase K collected on the VMXi beamline at the Diamond Light Source, UK
Multicrystal data of proteinase K collected on the VMXi beamline at the Diamond Light Source, UK Open
These are a series of datasets which have been collected on the VMXi beamline at the Diamond Light Source, UK. They were collected in-situ at room temperature using a Dectris 2X 4M detector. The datasets were collected in unattend…
View article: In-situ data recorded from thaumatin crystals on Diamond Light Source VMXi
In-situ data recorded from thaumatin crystals on Diamond Light Source VMXi Open
Data collected as part of routine beamline commissioning, from samples of thaumatin grown in 0.1 M Sodium citrate, 0.75M Sodium / Potassium tartrate. Data were collected in unattended mode with positions identified via SynchWeb fr…
View article: In-situ data recorded from thaumatin crystals on Diamond Light Source VMXi
In-situ data recorded from thaumatin crystals on Diamond Light Source VMXi Open
Data collected as part of routine beamline commissioning, from samples of thaumatin grown in 0.1 M Sodium citrate, 0.75M Sodium / Potassium tartrate. Data were collected in unattended mode with positions identified via SynchWeb fr…
View article: Updated example thaumatin data set from Diamond Light Source VMXi
Updated example thaumatin data set from Diamond Light Source VMXi Open
Example data set from the VMXi beamline at Diamond Light Source recorded in situ from a thaumatin crystal with processing results: For AUTOMATIC/DEFAULT/NATIVE Overall Low High High resolution limit 1.85 5.02 1.85 Low resolution limit 50.4…
View article: Updated example thaumatin data set from Diamond Light Source VMXi
Updated example thaumatin data set from Diamond Light Source VMXi Open
Example data set from the VMXi beamline at Diamond Light Source recorded in situ from a thaumatin crystal with processing results: For AUTOMATIC/DEFAULT/NATIVE Overall Low High High resolution limit 1.85 5.02 1.85 Low resolution limit 50.4…
View article: VMXi: a fully automated, fully remote, high-flux <i>in situ</i> macromolecular crystallography beamline
VMXi: a fully automated, fully remote, high-flux <i>in situ</i> macromolecular crystallography beamline Open
VMXi is a new high-flux microfocus macromolecular crystallography beamline at Diamond Light Source. The beamline, dedicated to fully automated and fully remote data collection of macromolecular crystals in situ , allows rapid screening of …
View article: A high-throughput crystallisation facility interacting with the new VMXi beamline at the Diamond Light Source
A high-throughput crystallisation facility interacting with the new VMXi beamline at the Diamond Light Source Open
View article: First steps towards routine serial crystallography at the VMXi beamline
First steps towards routine serial crystallography at the VMXi beamline Open
In protein crystallisation it is not uncommon for contami-
View article: A subfibrillar deformation mechanism in corneal collagen that affords flexibility
A subfibrillar deformation mechanism in corneal collagen that affords flexibility Open
Purpose : To elucidate the hierarchical deformation mechanisms of corneal collagen, and quantify the contribution of a subfibrillar “spring-like” mechanism associated with the straightening of tropocollagen molecules. Methods : Strips were…
View article: Example Data Set From Diamond Light Source Vmxi Beamline (Eiger 4M Data, Nexus Format)
Example Data Set From Diamond Light Source Vmxi Beamline (Eiger 4M Data, Nexus Format) Open
Data set recorded from Thermolysin crystal record in situ with Eiger 4M detector, to demonstrate file format used for this instrument at Diamond Light Source. Processing results using xia2 / DIALS: For AUTOMATIC/DEFAULT/SAD Overal…
View article: Example Data Set From Diamond Light Source Vmxi Beamline (Eiger 4M Data, Nexus Format)
Example Data Set From Diamond Light Source Vmxi Beamline (Eiger 4M Data, Nexus Format) Open
Data set recorded from Thermolysin crystal record in situ with Eiger 4M detector, to demonstrate file format used for this instrument at Diamond Light Source. Processing results using xia2 / DIALS: For AUTOMATIC/DEFAULT/SAD Overal…
View article: The hierarchical response of human corneal collagen to load
The hierarchical response of human corneal collagen to load Open
Collagen is the primary mediator of soft tissue biomechanics, and variations in its hierarchical structure convey the varying amounts of structural support necessary for organs to function normally. Here we have examined the structural res…
View article: Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering Open
Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector tech…
View article: A generic protocol for protein crystal dehydration using the HC1b humidity controller
A generic protocol for protein crystal dehydration using the HC1b humidity controller Open
Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and comple…
View article: Applications of thin-film sandwich crystallization platforms
Applications of thin-film sandwich crystallization platforms Open
Examples are shown of protein crystallization in, and data collection from, solutions sandwiched between thin polymer films using vapour-diffusion and batch methods. The crystallization platform is optimal for both visualization and in sit…
View article: Crystal Dehydration in Membrane Protein Crystallography
Crystal Dehydration in Membrane Protein Crystallography Open