Jun‐Xue Jin
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View article: Artificial intelligence-powered oocyte evaluation: Correlating cytoplasmic features with blastocyst development
Artificial intelligence-powered oocyte evaluation: Correlating cytoplasmic features with blastocyst development Open
Objective: This study aimed to establish a quantitative and interpretable method for assessing oocyte quality by analyzing cytoplasmic morphology and intensity features using artificial intelligence.Methods: A total of 695 oocyte images we…
View article: A long noncoding RNA with enhancer-like function in pig zygotic genome activation
A long noncoding RNA with enhancer-like function in pig zygotic genome activation Open
The zygotic genome activation (ZGA) is crucial for the development of pre-implantation embryos. Long noncoding RNAs (lncRNAs) play significant roles in many biological processes, but the study on their role in the early embryonic developme…
View article: CRISPR Ribonucleoprotein-Mediated Precise Editing of Multiple Genes in Porcine Fibroblasts
CRISPR Ribonucleoprotein-Mediated Precise Editing of Multiple Genes in Porcine Fibroblasts Open
The multi-gene editing porcine cell model can analyze the genetic mechanisms of multiple genes, which is beneficial for accelerating genetic breeding. However, there has been a lack of an effective strategy to simultaneously perform precis…
View article: SINE‐Associated LncRNA <i>SAWPA</i> Regulates Porcine Zygotic Genome Activation
SINE‐Associated LncRNA <i>SAWPA</i> Regulates Porcine Zygotic Genome Activation Open
In mice, retrotransposon‐associated long noncoding RNAs (lncRNA) play important regulatory roles in pre‐implantation development; however, it is largely unknown whether they function in the pre‐implantation development in pigs. The current…
View article: Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript
Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript Open
Simultaneously, multiplexed genome engineering and targeting multiple genomic loci are valuable to elucidating gene interactions and characterizing genetic networks that affect phenotypes. Here, we developed a general CRISPR-based platform…
View article: Multiplexed genome engineering for porcine fetal fibroblasts with gRNA–tRNA arrays based on CRISPR/Cas9
Multiplexed genome engineering for porcine fetal fibroblasts with gRNA–tRNA arrays based on CRISPR/Cas9 Open
Multiplex gene modifications are highly required for various fields of porcine research. In many species, the CRISPR/Cas9 system has been widely applied for genomic editing and provides a potential tool for introducing multiplex genome mut…
View article: Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes
Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes Open
Tannin (TA) improves porcine oocyte cytoplasmic maturation and subsequent embryonic development after in vitro fertilization (IVF). However, the mechanism through which TA blocks polyspermy after IVF remains unclear. Hence, the biological …
View article: Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes
Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes Open
This study aimed to determine the underlying mechanism of ramelteon on the competence of oocyte and subsequent embryo development in pigs during in vitro maturation (IVM). Our results showed that the cumulus expansion index was significant…
View article: Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2
Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2 Open
Previous studies suggest that the inclusion of melatonin (MTn) in in vitro maturation protocols improves the developmental competence of oocytes by scavenging reactive oxygen species (ROS). However, the molecular mechanisms integrating mel…
View article: Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs
Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs Open
To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations of TA (0, 1, 10 and 100 μg/mL) were supplemented with a maturation medium and the COCs and subsequent embryonic development we…
View article: Conservation and divergence of poly(A) tail regulation during the mammalian oocyte-to-embryo transition
Conservation and divergence of poly(A) tail regulation during the mammalian oocyte-to-embryo transition Open
SUMMARY Poly(A) tail length and non-A residues are vital for oocyte-to-embryo transition (OET) in mice and humans 1–5 . However, the role of poly(A) tail length and non-A residues during OET in other commonly used mammalian animal models f…
View article: Melatonin Receptor 2 Promotes Lipid Metabolism of Cumulus-oocyte Complexes via the cAMP/PKA Pathway
Melatonin Receptor 2 Promotes Lipid Metabolism of Cumulus-oocyte Complexes via the cAMP/PKA Pathway Open
Background: The importance of the processes of lipogenesis and lipolysis in providing an essential energy source during oocyte maturation is increasingly being recognized. Recent our studies have demonstrated that melatonin up-regulated li…
View article: Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system
Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system Open
Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed ce…
View article: Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig
Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig Open
The inner cell mass (ICM) in blastocyst is the origin of all somatic and germ cells in mammals and pluripotent stem cells (PSCs) in vitro. As the conserved principles between pig and human, here we performed comprehensive single‐cell RNA‐s…
View article: The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells
The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells Open
The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly used in applicatio…
View article: Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer
Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer Open
This study was carried out to examine the effects of manganese (Mn) on the developmental competence of porcine oocytes during in vitro maturation (IVM) after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT). Upon tr…
View article: Improved early development of porcine cloned embryos by treatment with quisinostat, a potent histone deacetylase inhibitor
Improved early development of porcine cloned embryos by treatment with quisinostat, a potent histone deacetylase inhibitor Open
Recently, the modification of the epigenetic status of somatic cell nuclear transfer (SCNT) embryos by treatment with histone deacetylase inhibitors (HDACis) has made it possible to alter epigenetic traits and improve the developmental com…
View article: Stimulatory Effects of Melatonin on Porcine In Vitro Maturation Are Mediated by MT2 Receptor
Stimulatory Effects of Melatonin on Porcine In Vitro Maturation Are Mediated by MT2 Receptor Open
Melatonin is a multifunctional molecule with numerous biological activities. The fact that melatonin modulates the functions of porcine granulosa cells via the MT2 receptor suggests the possibility of MT2 receptor-mediation for melatonin t…
View article: Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer Open
Due to their similarities with humans in anatomy, physiology, and genetics miniature pigs are becoming an attractive model for biomedical research. We aim to establish and evaluate blood type O cells derived from Korean native pig (KNP), a…
View article: The HDAC Inhibitor LAQ824 Enhances Epigenetic Reprogramming and In Vitro Development of Porcine SCNT Embryos
The HDAC Inhibitor LAQ824 Enhances Epigenetic Reprogramming and In Vitro Development of Porcine SCNT Embryos Open
Background/Aims: Hypoacetylation caused by aberrant epigenetic nuclear reprogramming results in low efficiency of mammalian somatic cell nuclear transfer (SCNT). Many epigenetic remodeling drugs have been used in attempts to improve in vit…
View article: Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression
Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression Open
Soluble human tumor necrosis factor (shTNFRI-Fc) and human heme oxygenase 1 (hHO-1) are key regulators for protection against oxidative and inflammatory injury for xenotransplantation. Somatic cells with more than 10 copy numbers of shTNFR…
View article: Mineralized deposits in the uterus of a pig without pregnancy loss
Mineralized deposits in the uterus of a pig without pregnancy loss Open
Herein, we describe a case of uterine calcification in the uterus of a pig without pregnancy loss. The recipient underwent cloned embryo transfer and Cesarean section for safe delivery of cloned piglets. During the Cesarean section, 4 whit…