Julia R. Lazzari-Dean
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View article: BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system
BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system Open
The discovery of bifunctional degradation activating compounds (BiDACs) has led to the development of a new class of drugs that promote the clearance of their protein targets. BiDAC-induced ubiquitination is generally believed to direct cy…
View article: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Author response: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Author response: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
View article: Author response: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Author response: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
View article: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Identifying FUS amyotrophic lateral sclerosis disease signatures in patient dermal fibroblasts
Identifying FUS amyotrophic lateral sclerosis disease signatures in patient dermal fibroblasts Open
Amyotrophic lateral sclerosis (ALS) is a rapidly progressing, highly heterogeneous neurodegenerative disease, underscoring the importance of obtaining information to personalize clinical decisions quickly after diagnosis. Here, we investig…
View article: Morphological and genetic screens reveal mechanisms of BiDAC-induced plasma membrane protein degradation
Morphological and genetic screens reveal mechanisms of BiDAC-induced plasma membrane protein degradation Open
The discovery of bifunctional degradation activating compounds (BiDACs) has led to the development of a new class of drugs that promote the clearance of their protein targets. BiDAC-induced ubiquitination is generally believed to direct cy…
View article: A red-emitting carborhodamine for monitoring and measuring membrane potential
A red-emitting carborhodamine for monitoring and measuring membrane potential Open
Biological membrane potentials, or voltages, are a central facet of cellular life. Optical methods to visualize cellular membrane voltages with fluorescent indicators are an attractive complement to traditional electrode-based approaches, …
View article: From cameras to confocal to cytometry: measuring tumbling rates is a general way to reveal protein binding - Raw Data
From cameras to confocal to cytometry: measuring tumbling rates is a general way to reveal protein binding - Raw Data Open
Molecular interactions are central to understanding any biological system, but they are labor-intensive to measure with existing optical approaches. Tracking "tumbling" (rotational diffusion) is a generalizable strategy for determining par…
View article: From cameras to confocal to cytometry: measuring tumbling rates is a general way to reveal protein binding - Raw Data
From cameras to confocal to cytometry: measuring tumbling rates is a general way to reveal protein binding - Raw Data Open
Molecular interactions are central to understanding any biological system, but they are labor-intensive to measure with existing optical approaches. Tracking "tumbling" (rotational diffusion) is a generalizable strategy for determining par…
View article: A red-emitting carborhodamine for monitoring and measuring membrane potential
A red-emitting carborhodamine for monitoring and measuring membrane potential Open
Biological membrane potentials, or voltages, are a central facet of cellular life. Optical methods to visualize cellular membrane voltages with fluorescent indicators are an attractive complement to traditional electrode-based approaches, …
View article: Magnetic control of GFP-like fluorescent proteins
Magnetic control of GFP-like fluorescent proteins Open
Zenodo hosts the archival version of this document; for convenient viewing, please visit andrewgyork.github.io/gfp_magnetofluorescence Abstract We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fl…
View article: Magnetic control of GFP-like fluorescent proteins - Raw data 2
Magnetic control of GFP-like fluorescent proteins - Raw data 2 Open
We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetor…
View article: Magnetic control of GFP-like fluorescent proteins - Raw data 1
Magnetic control of GFP-like fluorescent proteins - Raw data 1 Open
We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetor…
View article: Magnetic control of GFP-like fluorescent proteins - Raw data 1
Magnetic control of GFP-like fluorescent proteins - Raw data 1 Open
We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetor…
View article: Magnetic control of GFP-like fluorescent proteins - Raw data 2
Magnetic control of GFP-like fluorescent proteins - Raw data 2 Open
We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetor…
View article: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome
Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Author Response: Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome
Author Response: Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Reviewer #1 (Public Review): Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome
Reviewer #1 (Public Review): Impairment of lipid homoeostasis causes accumulation of protein aggregates in the lysosome Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption
Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption Open
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, …
View article: A scalable screening platform for phenotypic subtyping of ALS patient-derived fibroblasts
A scalable screening platform for phenotypic subtyping of ALS patient-derived fibroblasts Open
A major challenge for understanding and treating Amyotrophic Lateral Sclerosis (ALS) is that most patients have no known genetic cause. Even within defined genetic subtypes, patients display considerable clinical heterogeneity. It is uncle…
View article: mScarlet fluorescence lifetime reports lysosomal pH with high precision
mScarlet fluorescence lifetime reports lysosomal pH with high precision Open
This is a scientific publication describing the use of mScarlet lifetime as a pH indicator tuned for acidic compartments. Here, we explore the application of this tool to quantify pH in the mammalian lysosome. Abstract: The lysosome mainta…
View article: mScarlet fluorescence lifetime reports lysosomal pH with high precision
mScarlet fluorescence lifetime reports lysosomal pH with high precision Open
This is a scientific publication describing the use of mScarlet lifetime as a pH indicator tuned for acidic compartments. Here, we explore the application of this tool to quantify pH in the mammalian lysosome. Abstract: The lysosome mainta…
View article: mScarlet fluorescence lifetime reports lysosomal pH quantitatively
mScarlet fluorescence lifetime reports lysosomal pH quantitatively Open
This is a scientific publication describing the use of mScarlet lifetime as a pH indicator tuned for acidic compartments. Here, we explore the application of this tool to quantify pH in the mammalian lysosome. Abstract: The lysosome mainta…
View article: Raw data supporting "mScarlet fluorescence lifetime reports lysosomal pH quantitatively"
Raw data supporting "mScarlet fluorescence lifetime reports lysosomal pH quantitatively" Open
Original dataset and processing code supporting the preprint (scientific publication) "mScarlet fluorescence lifetime reports lysosomal pH quantitatively." Publication Abstract: The lysosome maintains a highly acidic pH, which is critical …
View article: Raw data supporting "mScarlet fluorescence lifetime reports lysosomal pH quantitatively"
Raw data supporting "mScarlet fluorescence lifetime reports lysosomal pH quantitatively" Open
Original dataset and processing code supporting the preprint (scientific publication) "mScarlet fluorescence lifetime reports lysosomal pH quantitatively." Publication Abstract: The lysosome maintains a highly acidic pH, which is critical …
View article: A Phenotypic Spectrum Between ALS and Health in Patient-Derived Fibroblasts Enables Subgrouping of FUS and Sporadic ALS and Evaluation of ASO Treatment
A Phenotypic Spectrum Between ALS and Health in Patient-Derived Fibroblasts Enables Subgrouping of FUS and Sporadic ALS and Evaluation of ASO Treatment Open
View article: Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy
Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy Open
Background: Membrane potential (V mem) exerts physiological influence across a wide range of time and space scales. To study V mem in these diverse contexts, it is essential to accurately r…
View article: Measuring Absolute Membrane Potential Across Space and Time
Measuring Absolute Membrane Potential Across Space and Time Open
Membrane potential (V mem ) is a fundamental biophysical signal present in all cells. V mem signals range in time from milliseconds to days, and they span lengths from microns to centimeters. V mem affects many cellular processes, ranging …