Julian Riba
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View article: Single-cell RNA sequencing reveals homogeneous transcriptome patterns and low variance in a suspension CHO-K1 and an adherent HEK293FT cell line in culture conditions
Single-cell RNA sequencing reveals homogeneous transcriptome patterns and low variance in a suspension CHO-K1 and an adherent HEK293FT cell line in culture conditions Open
Recombinant mammalian host cell lines, in particular CHO and HEK293 cells, are used for the industrial production of therapeutic proteins. Despite their well-known genomic instability, the control mechanisms that enable cells to respond to…
View article: Printing Microbial Dark Matter: Using Single Cell Dispensing and Genomics to Investigate the Patescibacteria/Candidate Phyla Radiation
Printing Microbial Dark Matter: Using Single Cell Dispensing and Genomics to Investigate the Patescibacteria/Candidate Phyla Radiation Open
As of today, the majority of environmental microorganisms remain uncultured. They are therefore referred to as “microbial dark matter.” In the recent past, cultivation-independent methods like single-cell genomics (SCG) enabled the discove…
View article: Blastic transformation of <i>BCR‐ABL1</i> positive chronic myeloid leukaemia through acquisition of <i>CBFB‐MYH11</i> and mutant <i>KIT</i>
Blastic transformation of <i>BCR‐ABL1</i> positive chronic myeloid leukaemia through acquisition of <i>CBFB‐MYH11</i> and mutant <i>KIT</i> Open
Chromosomal aberrations in addition to BCR-ABL1 at diagnosis of chronic myeloid leukaemia (CML) in chronic phase (CP-CML) occur in ~5–12% of patients.1-3 Progression to blast phase (BP) is accompanied by genetic evolution with concomitant …
View article: Rapid, Untargeted Chemical Profiling of Single Cells in Their Native Environment
Rapid, Untargeted Chemical Profiling of Single Cells in Their Native Environment Open
We report a method that enables untargeted, high throughput, and quantitative mass spectrometric analysis of single cells from cell suspension without needing additional sample preparation procedures (e.g., molecular tagging) through the c…
View article: Gene mutations and clonal architecture in myelodysplastic syndromes and changes upon progression to acute myeloid leukaemia and under treatment
Gene mutations and clonal architecture in myelodysplastic syndromes and changes upon progression to acute myeloid leukaemia and under treatment Open
Summary Knowledge of the molecular and clonal characteristics in the myelodysplastic syndromes ( MDS ) and during progression to acute myeloid leukaemia ( AML ) is essential to understand the disease dynamics and optimize treatment. Sequen…
View article: Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing
Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing Open
Intratumoral genetic heterogeneity may impact disease outcome. Gold standard for dissecting clonal heterogeneity are single-cell analyses. Here, we present an efficient workflow based on an advanced Single-Cell Printer (SCP) device for the…