Julianna J. Tomlinson
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View article: Elevated brain α-synuclein, phosphorylated-tau, and oxidative stress in mice that survived influenza A pneumonitis
Elevated brain α-synuclein, phosphorylated-tau, and oxidative stress in mice that survived influenza A pneumonitis Open
Influenza virus infection increases the incidence of parkinsonism in humans. We have previously shown that allelic variants at the Parkinson’s disease (PD)-linked Lrrk2 locus modulate host responses in vivo . Here, we asked whether Lrrk2 k…
View article: Optogenetic stimulation of nigral astrocytes is neuroprotective in a 6-OHDA model of neurodegeneration
Optogenetic stimulation of nigral astrocytes is neuroprotective in a 6-OHDA model of neurodegeneration Open
Summary Highlights Optogenetic stimulation of nigral astrocytes attenuates motor deficits & Th+ cell loss in a 6-OHDA model of neurodegeneration Bulk RNA-seq analysis reveals optogenetic stimulation of nigral astrocytes induces early chang…
View article: Variants in Lrrk2 and Snca deficiency do not alter the course of primary encephalitis due to neurotropic reovirus T3D in newborn mice
Variants in Lrrk2 and Snca deficiency do not alter the course of primary encephalitis due to neurotropic reovirus T3D in newborn mice Open
Variants at the leucine-rich repeat kinase-2 ( LRRK2 ) and α-synuclein ( SNCA ) loci are associated with Parkinson’s disease (PD) risk. Viral infections have also been linked to increased risk of developing PD. In exploring a role for each…
View article: Development of a simplified smell test to identify Parkinson’s disease using multiple cohorts, machine learning and item response theory
Development of a simplified smell test to identify Parkinson’s disease using multiple cohorts, machine learning and item response theory Open
View article: Race and ethnicity matter! Moving Parkinson’s risk research towards diversity and inclusiveness
Race and ethnicity matter! Moving Parkinson’s risk research towards diversity and inclusiveness Open
Parkinson's disease (PD) is a prevalent neurodegenerative disorder that shows considerable heterogeneity of risk factors however, the degree to which race/ethnicity has been actively pursued in PD risk research is unknown. We examined PD l…
View article: A Protocol for Neuralized Murine Olfactory Organoids
A Protocol for Neuralized Murine Olfactory Organoids Open
Chronic olfactory dysfunction can be associated with parkinsonism, dementia, demyelinating disorders and schizophrenia. The olfactory epithelium (OE) represents an interface between the environment and the central nervous system. Mounting …
View article: Variants in Lrrk2 and Snca deficiency do not alter the course of primary encephalitis due to neurotropic reovirus T3D in newborn mice
Variants in Lrrk2 and Snca deficiency do not alter the course of primary encephalitis due to neurotropic reovirus T3D in newborn mice Open
Variants of the leucine-rich repeat kinase-2 (LRRK2) and alpha-synuclein (SNCA) genes are associated with Parkinson's disease risk. We previously demonstrated that two Lrrk2 knock-in variants as well as Snca expression alter survival rates…
View article: Correction: Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19
Correction: Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19 Open
View article: Development of a Simplified Smell Test to Identify Patients with Typical Parkinson’s as Informed by Multiple Cohorts, Machine Learning and External Validation
Development of a Simplified Smell Test to Identify Patients with Typical Parkinson’s as Informed by Multiple Cohorts, Machine Learning and External Validation Open
Background Reduced olfaction is a common feature of patients with typical Parkinson disease (PD). We sought to develop and validate a simplified smell test as a screening tool to help identify PD patients and explore its differentiation fr…
View article: Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19
Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19 Open
The majority of patients with Parkinson disease (PD) experience a loss in their sense of smell and accumulate insoluble α-synuclein aggregates in their olfactory bulbs (OB). Subjects affected by a SARS-CoV-2-linked illness (COVID-19) also …
View article: Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy
Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy Open
View article: A topographical atlas of α-synuclein dosage and cell type-specific expression in adult mouse brain and peripheral organs
A topographical atlas of α-synuclein dosage and cell type-specific expression in adult mouse brain and peripheral organs Open
View article: Persistent Hyposmia as Surrogate for α-Synuclein-Linked Brain Pathology
Persistent Hyposmia as Surrogate for α-Synuclein-Linked Brain Pathology Open
Impaired olfaction can be associated with neurodegenerative disorders. We examined odor identification in newly diagnosed patients with parkinsonism and those at increased risk, measured olfactory performances longitudinally, and juxtapose…
View article: Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19
Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19 Open
Summary The majority of patients with Parkinson disease (PD) experience a loss in their sense of smell and accumulate insoluble α-synuclein aggregates in their olfactory bulbs (OB). Subjects affected by a SARS-CoV-2-linked illness (COVID-1…
View article: A topographical atlas of α-synuclein cell type-specific expression in adult mouse brain and peripheral organs
A topographical atlas of α-synuclein cell type-specific expression in adult mouse brain and peripheral organs Open
Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide and presents pathologically with Lewy pathology and dopaminergic neuron loss. Lewy pathology contains aggregated ⍺Synuclein (⍺Syn), a protein encoded by…
View article: Race and Ethnic Background in Parkinson’s Disease: A Literature Review and Risk Factor Analysis
Race and Ethnic Background in Parkinson’s Disease: A Literature Review and Risk Factor Analysis Open
Importance: Parkinson’s disease (PD) is a common, neurodegenerative disorder. Inclusion of ethno-racial groups is critical to expanding current understanding of PD risk factors, pathogenesis, and increasing generalizability across diverse …
View article: Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy
Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy Open
Parkinson's disease (PD) is a debilitating neurodegenerative disease characterized by the loss of midbrain dopaminergic neurons (DaNs) and the abnormal accumulation of α-Synuclein (α-Syn) protein. Currently, no treatment can slow nor halt …
View article: Olfactory Organoids v1
Olfactory Organoids v1 Open
This protocol enables obtaining 3D matrix-embedded olfactory organoids from KRT5+ olfactory horizontal basal cells. Resulting samples consist of hydrogel matrix domes with a volume of containing hollow spheres of olfactory epithelium. The …
View article: Mouse Olfactory Horizontal Basal Cell Culture v1
Mouse Olfactory Horizontal Basal Cell Culture v1 Open
This protocol describes isolation and culture of basal cells from the olfactory epithelium of mice. The process involves serial enzymatic treatment of dissected olfactory epithelia and subsequent plating in commercial airway medium with du…
View article: Parkin coregulates glutathione metabolism in adult mammalian brain
Parkin coregulates glutathione metabolism in adult mammalian brain Open
We recently discovered that the expression of PRKN , a young-onset Parkinson disease-linked gene, confers redox homeostasis. To further examine the protective effects of parkin in an oxidative stress model, we first combined the loss of pr…
View article: Evaluation of the PREDIGT score’s performance in identifying newly diagnosed Parkinson’s patients without motor examination
Evaluation of the PREDIGT score’s performance in identifying newly diagnosed Parkinson’s patients without motor examination Open
Several recent publications described algorithms to identify subjects with Parkinson’s disease (PD). In creating the “PREDIGT Score”, we previously developed a hypothesis-driven, simple-to-use formula to potentially calculate the incidence…
View article: Immunohistochemistry Protocol for Paraffin-Embedded Sections - General v1
Immunohistochemistry Protocol for Paraffin-Embedded Sections - General v1 Open
This protocol details general immunohistochemistry procedure.
View article: Genetic and pharmacological reduction of CDK14 mitigates α-synuclein pathology in human neurons and in rodent models of Parkinson’s disease
Genetic and pharmacological reduction of CDK14 mitigates α-synuclein pathology in human neurons and in rodent models of Parkinson’s disease Open
Parkinsona’s disease (PD) is a debilitating neurodegenerative disease characterized by the loss of midbrain dopaminergic neurons (DaNs) and the abnormal accumulation of α-Synuclein (α-Syn) protein. Currently, no treatment can slow nor halt…
View article: BATL: Bayesian annotations for targeted lipidomics
BATL: Bayesian annotations for targeted lipidomics Open
Motivation Bioinformatic tools capable of annotating, rapidly and reproducibly, large, targeted lipidomic datasets are limited. Specifically, few programs enable high-throughput peak assessment of liquid chromatography–electrospray ionizat…
View article: Alpha-synuclein research: defining strategic moves in the battle against Parkinson’s disease
Alpha-synuclein research: defining strategic moves in the battle against Parkinson’s disease Open
With the advent of the genetic era in Parkinson’s disease (PD) research in 1997, α-synuclein was identified as an important player in a complex neurodegenerative disease that affects >10 million people worldwide. PD has been estimated to h…
View article: BATL: Bayesian annotations for targeted lipidomics
BATL: Bayesian annotations for targeted lipidomics Open
Motivation Bioinformatic tools capable of annotating, rapidly and reproducibly, large, targeted lipidomic datasets are limited. Specifically, few programs enable high-throughput peak assessment of liquid chromatography-electrospray ionizat…
View article: Age-associated insolubility of parkin in human midbrain is linked to redox balance and sequestration of reactive dopamine metabolites
Age-associated insolubility of parkin in human midbrain is linked to redox balance and sequestration of reactive dopamine metabolites Open
The mechanisms by which parkin protects the adult human brain from Parkinson disease remain incompletely understood. We hypothesized that parkin cysteines participate in redox reactions and that these are reflected in its posttranslational…
View article: Evaluation of the PREDIGT Score in Discriminating Parkinson Disease from Neurological Health
Evaluation of the PREDIGT Score in Discriminating Parkinson Disease from Neurological Health Open
Background We previously created the PREDIGT Score as an algorithm to predict the incidence of Parkinson disease. The model rests on a hypothesis-driven formula, [P R =(E+D+I)xGxT], that uses numerical values for five categories known to m…
View article: Age-Associated Insolubility of Parkin in Human Midbrain is Linked to Redox Balance and Sequestration of Reactive Dopamine Metabolites
Age-Associated Insolubility of Parkin in Human Midbrain is Linked to Redox Balance and Sequestration of Reactive Dopamine Metabolites Open
The mechanisms by which parkin protects the adult human brain from Parkinson disease remain incompletely understood. We hypothesized that parkin cysteines participate in redox reactions, which are reflected in its posttranslational modific…
View article: Parkinson Disease-Linked Parkin Mediates Redox Reactions That Lower Oxidative Stress In Mammalian Brain
Parkinson Disease-Linked Parkin Mediates Redox Reactions That Lower Oxidative Stress In Mammalian Brain Open
SUMMARY We recently hypothesized that parkin plays a role in redox homeostasis and provided evidence that it directly reduces hydrogen peroxide (H 2 O 2 ) in vitro . Here, we examined this anti-oxidant activity in vivo . Informed by findin…