Juergen Loeffler
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View article: Molecular diagnostics in cancer patients with suspected respiratory mold infections
Molecular diagnostics in cancer patients with suspected respiratory mold infections Open
Invasive fungal infections (IFIs), including aspergillosis and mucormycosis, are serious complications in cancer patients. Reliable detection of etiologic agents is challenging but important for optimal patient management. In a prospective…
View article: Real-time and digital PCR assays for the detection of <i>Aspergillus</i> DNA in contrived and clinical samples—a bi-center evaluation
Real-time and digital PCR assays for the detection of <i>Aspergillus</i> DNA in contrived and clinical samples—a bi-center evaluation Open
Aspergillus real-time PCR (qPCR) is well-established, but limitations persist. These may be overcome by digital PCR (dPCR) technology, with its potential to be more analytically sensitive, more precise, and less affected by inhibition comp…
View article: Unveiling immune interference: how the dendritic cell response to co-infection with <i>Aspergillus fumigatus</i> is modulated by human cytomegalovirus and its virokine <sub>CMV</sub> IL-10
Unveiling immune interference: how the dendritic cell response to co-infection with <i>Aspergillus fumigatus</i> is modulated by human cytomegalovirus and its virokine <sub>CMV</sub> IL-10 Open
Human cytomegalovirus (HCMV) is a master of immune evasion and a potent modulator of the human immune system. The best-characterized mechanism employed by HCMV to suppress host immunity is the production of a viral interleukin-10 homolog (…
View article: Pilot Study Comparing the In Vitro Response of Circulating Monocytes to Aspergillus fumigatus Swollen Conidia in Patients with Chronic Graft-Versus-Host Disease and Healthy Volunteers
Pilot Study Comparing the In Vitro Response of Circulating Monocytes to Aspergillus fumigatus Swollen Conidia in Patients with Chronic Graft-Versus-Host Disease and Healthy Volunteers Open
Invasive fungal disease (IFD) is a recognised and potentially life-threatening complication of chronic graft-versus-host disease (cGVHD) and its treatment. Invasive aspergillosis (IA), most often due to the species Aspergillus fumigatus, i…
View article: The diagnosis of mucormycosis by PCR in patients at risk: a systematic review and meta-analysis
The diagnosis of mucormycosis by PCR in patients at risk: a systematic review and meta-analysis Open
View article: CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan
CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan Open
Invasive aspergillosis causes significant morbidity and mortality in immunocompromised patients. Natural killer (NK) cells are pivotal for antifungal defense. Thus far, CD56 is the only known pathogen recognition receptor on NK cells trigg…
View article: Polymerase Chain Reaction on Respiratory Tract Specimens of Immunocompromised Patients to Diagnose <i>Pneumocystis</i> Pneumonia: A Systematic Review and Meta-analysis
Polymerase Chain Reaction on Respiratory Tract Specimens of Immunocompromised Patients to Diagnose <i>Pneumocystis</i> Pneumonia: A Systematic Review and Meta-analysis Open
Background This meta-analysis examines the comparative diagnostic performance of polymerase chain reaction (PCR) for the diagnosis of Pneumocystis pneumonia (PCP) on different respiratory tract samples, in both human immunodeficiency virus…
View article: The Diagnosis of Mucormycosis by PCR: A Systematic Review and Meta-Analysis
The Diagnosis of Mucormycosis by PCR: A Systematic Review and Meta-Analysis Open
View article: The 49th Annual Meeting of the European Society for Blood and Marrow Transplantation: Van Bekkum Awards
The 49th Annual Meeting of the European Society for Blood and Marrow Transplantation: Van Bekkum Awards Open
View article: An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group
An Overview of Systematic Reviews of Polymerase Chain Reaction (PCR) for the Diagnosis of Invasive Aspergillosis in Immunocompromised People: A Report of the Fungal PCR Initiative (FPCRI)—An ISHAM Working Group Open
This overview of reviews (i.e., an umbrella review) is designed to reappraise the validity of systematic reviews (SRs) and meta-analyses related to the performance of Aspergillus PCR tests for the diagnosis of invasive aspergillosis in imm…
View article: The Use of Host Biomarkers for the Management of Invasive Fungal Disease
The Use of Host Biomarkers for the Management of Invasive Fungal Disease Open
Invasive fungal disease (IFD) causes severe morbidity and mortality, and the number of IFD cases is increasing. Exposure to opportunistic fungal pathogens is inevitable, but not all patients with underlying diseases increasing susceptibili…
View article: Defective antifungal immunity in patients with COVID-19
Defective antifungal immunity in patients with COVID-19 Open
The COVID-19 pandemic has placed a huge strain on global healthcare and been a significant cause of increased morbidity and mortality, particularly in at-risk populations. This disease attacks the respiratory systems and causes significant…
View article: Molecular Profiling Reveals Characteristic and Decisive Signatures in Patients after Allogeneic Stem Cell Transplantation Suffering from Invasive Pulmonary Aspergillosis
Molecular Profiling Reveals Characteristic and Decisive Signatures in Patients after Allogeneic Stem Cell Transplantation Suffering from Invasive Pulmonary Aspergillosis Open
Despite available diagnostic tests and recent advances, diagnosis of pulmonary invasive aspergillosis (IPA) remains challenging. We performed a longitudinal case-control pilot study to identify host-specific, novel, and immune-relevant mol…
View article: Chronic Occupational Mold Exposure Drives Expansion of Aspergillus-Reactive Type 1 and Type 2 T-Helper Cell Responses
Chronic Occupational Mold Exposure Drives Expansion of Aspergillus-Reactive Type 1 and Type 2 T-Helper Cell Responses Open
Occupational mold exposure can lead to Aspergillus-associated allergic diseases including asthma and hypersensitivity pneumonitis. Elevated IL-17 levels or disbalanced T-helper (Th) cell expansion were previously linked to Aspergillus-asso…
View article: Development of a Simple and Robust Whole Blood Assay with Dual Co-Stimulation to Quantify the Release of T-Cellular Signature Cytokines in Response to Aspergillus fumigatus Antigens
Development of a Simple and Robust Whole Blood Assay with Dual Co-Stimulation to Quantify the Release of T-Cellular Signature Cytokines in Response to Aspergillus fumigatus Antigens Open
Deeper understanding of mold-induced cytokine signatures could promote advances in the diagnosis and treatment of invasive mycoses and mold-associated hypersensitivity syndromes. Currently, most T-cellular immunoassays in medical mycology …
View article: CcpA- and Shm2-Pulsed Myeloid Dendritic Cells Induce T-Cell Activation and Enhance the Neutrophilic Oxidative Burst Response to Aspergillus fumigatus
CcpA- and Shm2-Pulsed Myeloid Dendritic Cells Induce T-Cell Activation and Enhance the Neutrophilic Oxidative Burst Response to Aspergillus fumigatus Open
Aspergillus fumigatus causes life-threatening opportunistic infections in immunocompromised patients. As therapeutic outcomes of invasive aspergillosis (IA) are often unsatisfactory, the development of targeted immunotherapy remains an imp…
View article: Unconventional T cells – New players in antifungal immunity
Unconventional T cells – New players in antifungal immunity Open
View article: Diagnostic Performance of (1→3)-β-D-Glucan Alone and in Combination with Aspergillus PCR and Galactomannan in Serum of Pediatric Patients after Allogeneic Hematopoietic Stem Cell Transplantation
Diagnostic Performance of (1→3)-β-D-Glucan Alone and in Combination with Aspergillus PCR and Galactomannan in Serum of Pediatric Patients after Allogeneic Hematopoietic Stem Cell Transplantation Open
Data on biomarker-assisted diagnosis of invasive aspergillosis (IA) in pediatric patients is scarce. Therefore, we conducted a cohort study over two years including 404 serum specimens of 26 pediatric patients after allogeneic hematopoieti…
View article: <i>Aspergillus</i> Polymerase Chain Reaction—An Update on Technical Recommendations, Clinical Applications, and Justification for Inclusion in the Second Revision of the EORTC/MSGERC Definitions of Invasive Fungal Disease
<i>Aspergillus</i> Polymerase Chain Reaction—An Update on Technical Recommendations, Clinical Applications, and Justification for Inclusion in the Second Revision of the EORTC/MSGERC Definitions of Invasive Fungal Disease Open
Aspergillus polymerase chain reaction testing of blood and respiratory samples has recently been included in the second revision of the EORTC/MSGERC definitions for classifying invasive fungal disease. This is a result of considerable effo…
View article: Polymorphisms within the TNFSF4 and MAPKAPK2 Loci Influence the Risk of Developing Invasive Aspergillosis: A Two-Stage Case Control Study in the Context of the aspBIOmics Consortium
Polymorphisms within the TNFSF4 and MAPKAPK2 Loci Influence the Risk of Developing Invasive Aspergillosis: A Two-Stage Case Control Study in the Context of the aspBIOmics Consortium Open
Here, we assessed whether 36 single nucleotide polymorphisms (SNPs) within the TNFSF4 and MAPKAPK2 loci influence the risk of developing invasive aspergillosis (IA). We conducted a two-stage case control study including 911 high-risk patie…
View article: Multiplex PCR Based Strategy for Detection of Fungal Pathogen DNA in Patients with Suspected Invasive Fungal Infections
Multiplex PCR Based Strategy for Detection of Fungal Pathogen DNA in Patients with Suspected Invasive Fungal Infections Open
A new and easy polymerase chain reaction (PCR) multiplex strategy, for the identification of the most common fungal species involved in invasive fungal infections (IFI) was developed in this work. Two panels with species-specific markers w…
View article: Triple RNA-Seq Reveals Synergy in a Human Virus-Fungus Co-infection Model
Triple RNA-Seq Reveals Synergy in a Human Virus-Fungus Co-infection Model Open
High-throughput RNA sequencing (RNA-seq) is routinely applied to study diverse biological processes; however, when performed separately on interacting organisms, systemic noise intrinsic to RNA extraction, library preparation, and sequenci…
View article: Reconstituting NK Cells After Allogeneic Stem Cell Transplantation Show Impaired Response to the Fungal Pathogen Aspergillus fumigatus
Reconstituting NK Cells After Allogeneic Stem Cell Transplantation Show Impaired Response to the Fungal Pathogen Aspergillus fumigatus Open
Delayed natural killer (NK) cell reconstitution after allogeneic stem cell transplantation (alloSCT) is associated with a higher risk of developing invasive aspergillosis. The interaction of NK cells with the human pathogen Aspergillus …
View article: Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group
Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group Open
Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them particip…
View article: Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR
Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR Open
The mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium s…
View article: The Fungal PCR Initiative's evaluation of in-house and commercial Pneumocystis jirovecii qPCR assays: Toward a standard for a diagnostics assay
The Fungal PCR Initiative's evaluation of in-house and commercial Pneumocystis jirovecii qPCR assays: Toward a standard for a diagnostics assay Open
Quantitative real-time PCR (qPCR) is increasingly used to detect Pneumocystis jirovecii for the diagnosis of Pneumocystis pneumonia (PCP), but there are differences in the nucleic acids targeted, DNA only versus whole nucleic acid (WNA), a…
View article: An inter-laboratory multi-centre evaluation of the performance of Mucorales PCR assays when testing serum specimens: A study by the ISHAM Fungal PCR Initiative and the ModiMucor study group -FPCRI Mucor Laboratory Working group
An inter-laboratory multi-centre evaluation of the performance of Mucorales PCR assays when testing serum specimens: A study by the ISHAM Fungal PCR Initiative and the ModiMucor study group -FPCRI Mucor Laboratory Working group Open
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View article: Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people
Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people Open
BACKGROUND: This is an update of the original review published in the Cochrane Database of Systematic Reviews Issue 10, 2015.Invasive aspergillosis (IA) is the most common life-threatening opportunistic invasive mould infection in immunoco…
View article: Comparative Analysis of Inflammatory Cytokine Release and Alveolar Epithelial Barrier Invasion in a Transwell® Bilayer Model of Mucormycosis
Comparative Analysis of Inflammatory Cytokine Release and Alveolar Epithelial Barrier Invasion in a Transwell® Bilayer Model of Mucormycosis Open
Understanding the mechanisms of early invasion and epithelial defense in opportunistic mold infections is crucial for the evaluation of diagnostic biomarkers and novel treatment strategies. Recent studies revealed unique characteristics of…
View article: Immunomodulation as a Therapy for Aspergillus Infection: Current Status and Future Perspectives
Immunomodulation as a Therapy for Aspergillus Infection: Current Status and Future Perspectives Open
Invasive aspergillosis (IA) is the most serious life-threatening infectious complication of intensive remission induction chemotherapy and allogeneic stem cell transplantation in patients with a variety of hematological malignancies. Asper…