Jürgen Tomasch
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View article: Structural and regulatory determinants of flagellar motility in <i>Rhodobacterales</i> —the archetypal flagellum of <i>Phaeobacter inhibens</i> DSM 17395
Structural and regulatory determinants of flagellar motility in <i>Rhodobacterales</i> —the archetypal flagellum of <i>Phaeobacter inhibens</i> DSM 17395 Open
Flagellar motility is crucial for the swim-and-stick lifestyle and plays an important role in bacterial-algal interactions of Rhodobacterales . This alphaproteobacterial order contains three distinct types of flagellar gene clusters (FGCs)…
View article: Epigenetic cellular memory in <i>Pseudomonas aeruginosa</i> generates phenotypic variation in response to host environments
Epigenetic cellular memory in <i>Pseudomonas aeruginosa</i> generates phenotypic variation in response to host environments Open
Phenotypic diversification within pathogen populations can enhance survival in stressful environments, broaden niche colonization, and expand the ecological range of infectious diseases due to emerging collective pathogenicity characterist…
View article: Structural and regulatory determinants of flagellar motility in<i>Rhodobacterales</i>– The archetypal flagellum of<i>Phaeobacter inhibens</i>DSM 17395
Structural and regulatory determinants of flagellar motility in<i>Rhodobacterales</i>– The archetypal flagellum of<i>Phaeobacter inhibens</i>DSM 17395 Open
Flagellar motility is crucial for the swim-and-stick lifestyle of Rhodobacterales and plays an important role for bacterial-algal interactions. This alphaproteobacterial order contains three distinct types of flagellar gene clusters (FGCs)…
View article: Chemical quantification of N-acyl alanine methyl ester (NAME) production and impact on temporal gene expression patterns in Roseovarius tolerans EL-164
Chemical quantification of N-acyl alanine methyl ester (NAME) production and impact on temporal gene expression patterns in Roseovarius tolerans EL-164 Open
View article: Minimal transcriptional regulation of horizontally transferred photosynthesis genes in phototrophic bacterium <i>Gemmatimonas phototrophica</i>
Minimal transcriptional regulation of horizontally transferred photosynthesis genes in phototrophic bacterium <i>Gemmatimonas phototrophica</i> Open
The first phototrophic member of the bacterial phylum Gemmatimonadota , Gemmatimonas phototrophica AP64 T , received all its photosynthesis genes via distant horizontal gene transfer from a purple bacterium. Here, we investigated how these…
View article: On the evolution of chromosomal regions with high gene strand bias in bacteria
On the evolution of chromosomal regions with high gene strand bias in bacteria Open
On circular bacterial chromosomes, the majority of genes are coded on the leading strand. This gene strand bias (GSB) ranges from up to 85% in some Bacillota to a little more than 50% in other phyla. The factors determining the extent of t…
View article: On the Evolution of Chromosomal Regions with High Gene Strand Bias in Bacteria
On the Evolution of Chromosomal Regions with High Gene Strand Bias in Bacteria Open
On circular bacterial chromosomes, the majority of genes are coded on the leading strand. This gene strand bias (GSB) ranges from up to 85% in some Bacillota to little more than 50% in other phyla. The factors defining the extent of the GS…
View article: A photoheterotrophic bacterium from Iceland has adapted its photosynthetic machinery to the long days of polar summer
A photoheterotrophic bacterium from Iceland has adapted its photosynthetic machinery to the long days of polar summer Open
During their long evolution, anoxygenic phototrophic bacteria have inhabited a wide variety of natural habitats and developed specific strategies to cope with the challenges of any particular environment. Expression, assembly, and safe ope…
View article: The effect of site-specific recombinases XerCD on the removal of over-replicated chromosomal DNA through outer membrane vesicles in bacteria
The effect of site-specific recombinases XerCD on the removal of over-replicated chromosomal DNA through outer membrane vesicles in bacteria Open
Outer membrane vesicles (OMVs) are universally produced by Gram-negative bacteria and play important roles in symbiotic and pathogenic interactions. The DNA from the lumen of OMVs from the Alphaproteobacterium Dinoroseobacter shibae was pr…
View article: Role of XerCD in release of over-replicated DNA through Outer Membrane Vesicles in<i>Escherichia coli</i>
Role of XerCD in release of over-replicated DNA through Outer Membrane Vesicles in<i>Escherichia coli</i> Open
Outer membrane vesicles (OMVs) are universally produced by prokaryotes and play important roles in symbiotic and pathogenic interactions. Here we show that the Gammaproteobacterium Escherichia coli produces OMVs that contain DNA enriched f…
View article: Transcriptome Dynamics of Pseudomonas aeruginosa during Transition from Overlapping To Non-Overlapping Cell Cycles
Transcriptome Dynamics of Pseudomonas aeruginosa during Transition from Overlapping To Non-Overlapping Cell Cycles Open
The coordination of gene expression with the cell cycle has so far been studied only in a few bacteria, the bottleneck being the need for synchronized cultures. Here, we determined replication-associated effects on transcription by compari…
View article: The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica
The Influence of Calcium on the Growth, Morphology and Gene Regulation in Gemmatimonas phototrophica Open
The bacterium Gemmatimonas phototrophica AP64 isolated from a freshwater lake in the western Gobi Desert represents the first phototrophic member of the bacterial phylum Gemmatimonadota. This strain was originally cultured on agar plates b…
View article: Genomic epidemiology of clinical ESBL-producing Enterobacteriaceae in a German hospital suggests infections are primarily community- and regionally-acquired
Genomic epidemiology of clinical ESBL-producing Enterobacteriaceae in a German hospital suggests infections are primarily community- and regionally-acquired Open
Clinical Enterobacteriaceae isolates that produce extended-spectrum β-lactamases (ESBLs) have been increasingly reported at a global scale. However, comprehensive data on the molecular epidemiology of ESBL-producing strains are limited and…
View article: A bacterium from a mountain lake harvests light using both proton-pumping xanthorhodopsins and bacteriochlorophyll-based photosystems
A bacterium from a mountain lake harvests light using both proton-pumping xanthorhodopsins and bacteriochlorophyll-based photosystems Open
Photoheterotrophic bacteria harvest light energy using either proton-pumping rhodopsins or bacteriochlorophyll (BChl)-based photosystems. The bacterium Sphingomonas glacialis AAP5 isolated from the alpine lake Gossenköllesee contains genes…
View article: Transcriptome dynamics of<i>Pseudomonas aeruginosa</i>during transition from replication-uncoupled to -coupled growth
Transcriptome dynamics of<i>Pseudomonas aeruginosa</i>during transition from replication-uncoupled to -coupled growth Open
In bacteria, either chromosome duplication is coupled to cell division with only one replication round per cell cycle or DNA is replicated faster than the cells divide thus both processes are uncoupled. Here, we show that the opportunistic…
View article: Shared properties of gene transfer agent and core genes revealed by comparative genomics of Alphaproteobacteria
Shared properties of gene transfer agent and core genes revealed by comparative genomics of Alphaproteobacteria Open
Gene transfer agents (GTAs) are phage-like particles that transfer pieces of cellular genomic DNA to other cells. Homologues of the Rhodobacter capsulatus GTA (RcGTA) structural genes are widely distributed in the Alphaproteobacteria and p…
View article: The Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae
The Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae Open
Plasmids are small DNA molecules that replicate independently of the bacterial chromosome. The common view of the role of plasmids is dominated by the accumulation of resistance genes, which is responsible for the antibiotic crisis in heal…
View article: High plasmidome diversity of extended-spectrum beta-lactam-resistant Escherichia coli isolates collected during one year in one community hospital
High plasmidome diversity of extended-spectrum beta-lactam-resistant Escherichia coli isolates collected during one year in one community hospital Open
Plasmid-encoded antibiotic resistance encompasses many classes of currently used antibiotics. In globally distributed Escherichia coli lineages plasmids, which spread via horizontal gene transfer, are responsible for the dissemination of g…
View article: Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales
Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales Open
The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven …
View article: Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates Open
View article: The Influence of Genes on the “Killer Plasmid” of Dinoroseobacter shibae on Its Symbiosis With the Dinoflagellate Prorocentrum minimum
The Influence of Genes on the “Killer Plasmid” of Dinoroseobacter shibae on Its Symbiosis With the Dinoflagellate Prorocentrum minimum Open
The marine bacterium Dinoroseobacter shibae shows a Jekyll-and-Hyde behavior in co-culture with the dinoflagellate Prorocentrum minimum : In the initial symbiotic phase it provides the essential vitamins B12 (cobalamin) and B1 (thiamine) t…
View article: Orthodontic Forced Eruption of Permanent Anterior Teeth with Subgingival Fractures: A Systematic Review
Orthodontic Forced Eruption of Permanent Anterior Teeth with Subgingival Fractures: A Systematic Review Open
(1) Background: To assess orthodontic forced eruption (OFE) as a pre-restorative procedure for non-restorable permanent teeth with subgingival dental hard tissue defects after dental trauma. (2) Methods: A systematic electronic search of t…
View article: Distribution of cycle threshold values in RT-qPCR tests during the autumn 2020 peak of the COVID-19 pandemic in the Czech Republic
Distribution of cycle threshold values in RT-qPCR tests during the autumn 2020 peak of the COVID-19 pandemic in the Czech Republic Open
Reverse-transcription quantitative PCR (RT-qPCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19). We analysed 1927 samples coll…
View article: Connection Between Chromosomal Location and Function of CtrA Phosphorelay Genes in Alphaproteobacteria
Connection Between Chromosomal Location and Function of CtrA Phosphorelay Genes in Alphaproteobacteria Open
Most bacterial chromosomes are circular, with replication starting at one origin ( ori ) and proceeding on both replichores toward the terminus ( ter ). Several studies have shown that the location of genes relative to ori and ter can have…
View article: Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5
Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5 Open
An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria. Here, we report its description and pol…
View article: Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site <i>dif</i>
Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site <i>dif</i> Open
Gram-negative bacteria continually form vesicles from their outer membrane (outer membrane vesicles [OMVs]) during normal growth. OMVs frequently contain DNA, and it is unclear how DNA can be shuffled from the cytoplasm to the OMVs.
View article: Simultaneous Presence of Bacteriochlorophyll and Xanthorhodopsin Genes in a Freshwater Bacterium
Simultaneous Presence of Bacteriochlorophyll and Xanthorhodopsin Genes in a Freshwater Bacterium Open
Phototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins.
View article: Expression of the MexXY Aminoglycoside Efflux Pump and Presence of an Aminoglycoside-Modifying Enzyme in Clinical Pseudomonas aeruginosa Isolates Are Highly Correlated
Expression of the MexXY Aminoglycoside Efflux Pump and Presence of an Aminoglycoside-Modifying Enzyme in Clinical Pseudomonas aeruginosa Isolates Are Highly Correlated Open
The impact of MexXY efflux pump expression on aminoglycoside resistance in clinical Pseudomonas aeruginosa isolates has been debated. In this study, we found that, in general, elevated mexXY gene expression levels in clinical P. aeruginosa…
View article: Organism-specific depletion of highly abundant RNA species from bacterial total RNA
Organism-specific depletion of highly abundant RNA species from bacterial total RNA Open
High-throughput sequencing has become a standard tool for transcriptome analysis. The depletion of overrepresented RNA species from sequencing libraries plays a key role in establishing potent and cost-efficient RNA-seq routines. Commercia…
View article: Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth
Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth Open
Pseudomonas aeruginosa is an environmental bacterium and an opportunistic human pathogen. It is also a well-established model organism to study bacterial adaptation to stressful conditions, such as those encountered during an infect…