Explanipedia

Hydrophobin‐Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells Open

Hedar H. Al‐Terke, Grégory Beaune, Muhammad Junaid, Jani Seitsonen, Arja Paananen , et al. · 2025

Cancer is a leading cause of death globally. Early detection of tumors can be an effective way to reduce mortality, however, traditional cancer diagnostic methods are based on the detection of organ dysfunction, which generally occurs at a…

Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension Open

Noemi Gutierrez-Valdes, Francesc Cunyat, Juliette Balieu, Marie-Laure Walet-Balieu, Matthew J. Paul , et al. · 2024

Compressibility and porosity modulate the mechanical properties of giant gas vesicles Open

Hedar H. Al‐Terke, Grégory Beaune, Muhammad Junaid, Jani Seitsonen, Arja Paananen , et al. · 2023

Gas vesicles used as contrast agents for noninvasive ultrasound imaging must be formulated to be stable, and their mechanical properties must be assessed. We report here the formation of perfluoro- n -butane microbubbles coated with surfac…

Functional Magnetic Microdroplets for Antibody Extraction Open

Hedar H. Al‐Terke, Mika Latikka, Jaakko V. I. Timonen, L. Vékás, Arja Paananen , et al. · 2021

Antibodies play an essential role in modern medicine for diagnostic and therapeutic applications. Even though the production of antibodies is the fastest growing pharmaceutical industry area, the cost of antibodies remains high, which limi…

Editorial: Proceedings of ISPMF 2018 - Plant Molecular Farming Open

Anneli Ritala, Heiko Rischer, Suvi T. Häkkinen, Jussi J. Joensuu, Kirsi‐Marja Oksman‐Caldentey · 2020

EDITORIAL article Front. Plant Sci., 28 April 2020Sec. Plant Metabolism and Chemodiversity Volume 11 - 2020 | https://doi.org/10.3389/fpls.2020.00492

Hydrophobin-Protein A Fusion Protein Produced in Plants Efficiently Purified an Anti-West Nile Virus Monoclonal Antibody from Plant Extracts via Aqueous Two-Phase Separation Open

Collin Jugler, Jussi J. Joensuu, Qiang Chen · 2020

The development of monoclonal antibodies (mAbs) has provided vast opportunities to treat a wide range of diseases from cancer to viral infections. While plant-based production of mAbs has effectively lowered the upstream cost of mAb produc…

Self-Assembling Protein–Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding Open

Yingying Liu, Tarja K. Nevanen, Arja Paananen, Kristian Kempe, Paul Wilson , et al. · 2018

A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active…

Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production Open

Suvi T. Häkkinen, Lauri Reuter, Ninni Nuorti, Jussi J. Joensuu, Heiko Rischer , et al. · 2018

Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs derivin…

Cooperative colloidal self-assembly of metal-protein superlattice wires Open

Ville Liljeström, Ari Ora, Jukka Hassinen, Heikki Rekola, Nonappa Nonappa , et al. · 2017

In‐solution antibody harvesting with a plant‐produced hydrophobin–Protein A fusion Open

Katri Kurppa, Lauri Reuter, Anneli Ritala, Markus B. Linder, Jussi J. Joensuu · 2017

Summary Purification is a bottleneck and a major cost factor in the production of antibodies. We set out to engineer a bifunctional fusion protein from two building blocks, Protein A and a hydrophobin, aiming at low‐cost and scalable antib…

Elastic and pH-Responsive Hybrid Interfaces Created with Engineered Resilin and Nanocellulose Open

Wenwen Fang, Arja Paananen, Marika Vitikainen, Salla Koskela, Ann Westerholm‐Parvinen , et al. · 2017

We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its…

Novel Hydrophobin Fusion Tags for Plant-Produced Fusion Proteins Open

Lauri Reuter, Anneli Ritala, Markus B. Linder, Jussi J. Joensuu · 2016

Hydrophobin fusion technology has been applied in the expression of several recombinant proteins in plants. Until now, the technology has relied exclusively on the Trichoderma reesei hydrophobin HFBI. We screened eight novel hydrophobin ta…

Protein Bodies in Leaves Exchange Contents through the Endoplasmic Reticulum Open

Reza Saberianfar, Amirali Sattarzadeh, Jussi J. Joensuu, Susanne E. Kohalmi, Rima Menassa · 2016

Protein bodies (PBs) are organelles found in seeds whose main function is the storage of proteins that are used during germination for sustaining growth. PBs can also be induced to form in leaves when foreign proteins are produced at high …

Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers Open

Miika Soikkeli, Katri Kurppa, Markku Kainlauri, Sanna Arpiainen, Arja Paananen , et al. · 2016

We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor mo…

Physicochemical characterization of sahti, an ‘ancient’ beer style indigenous to Finland Open

Jukka Ekberg, Brian Gibson, Jussi J. Joensuu, Kristoffer Krogerus, Frederico Magalhães , et al. · 2015

Sahti, a strong, unhopped farmhouse beer flavoured with juniper, is still actively brewed in rural areas in Finland. Presented here is the first comprehensive analysis of the physical and chemical properties of this unique beer style. Twel…

Protein body formation in leaves of <i><span>N</span>icotiana benthamiana</i>: a concentration‐dependent mechanism influenced by the presence of fusion tags Open

Reza Saberianfar, Jussi J. Joensuu, Andrew Conley, Rima Menassa · 2015

Summary Protein bodies ( PB s) are endoplasmic reticulum ( ER ) derived organelles originally found in seeds whose function is to accumulate seed storage proteins. It has been shown that PB formation is not limited to seeds and green fluor…

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