Jyotsna Shah
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View article: <b>Morphological identification, prevalence, and infestation rate of <i>Varroa destructor</i> in <i>Apis dorsata</i> in Khyber Pakhtunkhwa, Pakistan</b>
<b>Morphological identification, prevalence, and infestation rate of <i>Varroa destructor</i> in <i>Apis dorsata</i> in Khyber Pakhtunkhwa, Pakistan</b> Open
Background: Apis dorsata is one of the most economically important bees. The A. dorsata colonies are rapidly declining in Pakistan due to multiple factors. Investigation regarding Varroa destructor in A. dorsata was completely ignored in t…
View article: Significance of Detecting Serum Antibodies to Outer Surface Protein A of Lyme Disease Borreliae in PCR-Confirmed Blood Infections
Significance of Detecting Serum Antibodies to Outer Surface Protein A of Lyme Disease Borreliae in PCR-Confirmed Blood Infections Open
Background/Objectives: Lyme disease is caused by some species of tick-borne bacteria of the genus Borrelia, termed Lyme disease Borreliae (LDB). Borrelia burgdorferi is the LDB species principally responsible for Lyme disease in the US. Th…
View article: Target Antigens in Western and Line Immunoblots for Supporting the Diagnosis of Lyme Disease. Comment on Porwancher et al. Immunoblot Criteria for Diagnosis of Lyme Disease: A Comparison of CDC Criteria to Alternative Interpretive Approaches. Pathogens 2023, 12, 1282
Target Antigens in Western and Line Immunoblots for Supporting the Diagnosis of Lyme Disease. Comment on Porwancher et al. Immunoblot Criteria for Diagnosis of Lyme Disease: A Comparison of CDC Criteria to Alternative Interpretive Approaches. Pathogens 2023, 12, 1282 Open
An article was recently published in Pathogens on using different target antigens from Borrelia species that cause Lyme disease for detecting serum antibodies to support a clinical diagnosis of Lyme disease (LD) [...]
View article: Recombinant protein immunoblots for differential diagnosis of tick-borne relapsing fever and Lyme disease
Recombinant protein immunoblots for differential diagnosis of tick-borne relapsing fever and Lyme disease Open
Lyme disease (LD) is caused by a group of tick-borne bacteria of the genus Borrelia termed Lyme disease Borreliae (LDB). The detection of serum antibodies to specific LDB antigens is widely used to support diagnosis of LD. Recent findings …
View article: Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases
Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases Open
Diagnosing and treating many infectious diseases depends on correctly identifying the causative pathogen. Characterization of pathogen-specific nucleic acid sequences by PCR is the most sensitive and specific method available for this purp…
View article: Dermatological and Genital Manifestations of Lyme Disease Including Morgellons Disease
Dermatological and Genital Manifestations of Lyme Disease Including Morgellons Disease Open
Although the erythema migrans (EM) skin rash is traditionally considered a hallmark of Lyme disease, other dermatological manifestations of the tickborne disease are less well known. We describe a 49-year-old woman with erosive genital ulc…
View article: Additional file 1 of IgG and IgM antibody formation to spike and nucleocapsid proteins in COVID-19 characterized by multiplex immunoblot assays
Additional file 1 of IgG and IgM antibody formation to spike and nucleocapsid proteins in COVID-19 characterized by multiplex immunoblot assays Open
Additional file 1. provides details of the calculations of the estimated clinical parameters of the detection of IgG and IgM antibodies in COVID-19 immunoblots.
View article: Combined Immunofluorescence (IFA) and Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Babesiosis in Patients from the USA, Europe and Australia
Combined Immunofluorescence (IFA) and Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Babesiosis in Patients from the USA, Europe and Australia Open
Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals worldwide. Human babesiosis is a predominantly zoonotic disease transmitted by hard ticks that is of increasing health concern in the USA and many other cou…
View article: A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis
A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis Open
Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid i…
View article: Lyme Disease: Diversity of Borrelia Species in California and Mexico Detected Using a Novel Immunoblot Assay
Lyme Disease: Diversity of Borrelia Species in California and Mexico Detected Using a Novel Immunoblot Assay Open
Background: With more than 300,000 new cases reported each year in the United States of America (USA), Lyme disease is a major public health concern. Borrelia burgdorferi sensu stricto (Bbss) is considered the primary agent of Lyme disease…
View article: <p>Classification and Staging of Morgellons Disease: Lessons from Syphilis</p>
Classification and Staging of Morgellons Disease: Lessons from Syphilis Open
The enhanced staging system provides objective criteria to assess the severity of dermopathy in MD. Further studies are needed to determine the optimal treatment for MD based on this staging system related to Borrelia infection.
View article: Line Immunoblot Assay for Tick-Borne Relapsing Fever and Findings in Patient Sera from Australia, Ukraine and the USA
Line Immunoblot Assay for Tick-Borne Relapsing Fever and Findings in Patient Sera from Australia, Ukraine and the USA Open
Tick-borne relapsing fever (TBRF) is caused by spirochete bacteria of the genus Borrelia termed relapsing fever Borreliae (RFB). TBRF shares symptoms with Lyme disease (LD) caused by related Lyme disease Borreliae (LDB). TBRF and LD are tr…
View article: Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques
Detection of tick-borne infection in Morgellons disease patients by serological and molecular techniques Open
The study demonstrates an association between MD and positive tests for both Bb and RFB spirochetes. In conjunction with previous studies, our study provides corroborative evidence linking MD to Borrelia infection and tick-borne illness.
View article: Relapsing fever <em>Borrelia</em> in California: a pilot serological study
Relapsing fever <em>Borrelia</em> in California: a pilot serological study Open
Results of this pilot study suggest that seroreactivity against Bb and RFB is widespread in California, and dual exposure to Bb and RFB may complicate the diagnosis of tick-borne disease. Greater awareness of RFB and broader screening for …
View article: Pilot Study of Immunoblots with Recombinant Borrelia burgdorferi Antigens for Laboratory Diagnosis of Lyme Disease
Pilot Study of Immunoblots with Recombinant Borrelia burgdorferi Antigens for Laboratory Diagnosis of Lyme Disease Open
Accurate laboratory diagnosis of Lyme disease (Lyme borreliosis), caused by the spirochete Borrelia burgdorferi (BB), is difficult and yet important to prevent serious disease. The US Centers for Disease Control and Prevention (CDC) presen…
View article: Fluorescent In Situ Hybridization: A Rapid diagnostic test for the detection and speciation of Mycobacterial infection
Fluorescent In Situ Hybridization: A Rapid diagnostic test for the detection and speciation of Mycobacterial infection Open
Background: A cost effective molecular based assay for the rapid detection and differentiation of MTB from NTM in resource poor countries is the need of the hour. Can FISH technique be considered as one such assay to meet the need of early…
View article: Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes
Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes Open
The MN Genus-MTBC dual probe fluorescence FISH assay previously applied to cultures can also be utilized in resource-limited tuberculosis-endemic countries for rapidly identifying and differentiating MTBC and NTM in sputum samples.
View article: Development of a sensitive PCR-dot blot assay to supplement serological tests for diagnosing Lyme disease
Development of a sensitive PCR-dot blot assay to supplement serological tests for diagnosing Lyme disease Open
Laboratory diagnosis of Lyme disease is difficult and presently dependent on detecting Borrelia burgdorferi-specific antibodies in patient serum with the disadvantage that the immune response to B. burgdorferi can be weak or variable, or a…
View article: Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures
Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures Open
Two rapid dual color fluorescence in situ hybridization (FISH) assays were evaluated for detecting M. tuberculosis and related pathogens in cultures. The MN Genus-MTBC FISH assay uses an orange fluorescent probe specific for the Mycobacter…
View article: Fluorescent In Situ Hybridization (FISH) for the detection and differentiation of mycobacterium tuberculosis and NTM in sputum and culture
Fluorescent In Situ Hybridization (FISH) for the detection and differentiation of mycobacterium tuberculosis and NTM in sputum and culture Open
Background: Early detection and differentiation between Mycobacterium tuberculosis (MTB) and the atypical Mycobacteria play an important role in the treatment and management of patients. We have recently developed ID-FISH Mycobacterium gen…