Kumar Binod
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View article: Mineralogy and texture of the eastern part of the Palung Granite, Lesser Himalaya, central Nepal
Mineralogy and texture of the eastern part of the Palung Granite, Lesser Himalaya, central Nepal Open
Geological mapping of the granite body along the Kulekhani-Palung area was carried out and the geological boundary of the granite body was well-traced out in 1:25,000 scales. This study investigates the mineralogical and textural character…
View article: Effect of BRCA1 on IFI16 and H2B acetylation during KSHV <i>de novo</i> infection.
Effect of BRCA1 on IFI16 and H2B acetylation during KSHV <i>de novo</i> infection. Open
(A) HMVEC-d cells electroporated with siC or siBRCA1 for 48 h were uninfected or infected by KSHV for 4 h and subjected to PLA using mouse anti-IFI16 and rabbit anti-acetyl lysine antibodies. The localization of IFI16-acetyl lysine (acetyl…
View article: Effect of Tsg101 knockdown on KSHV entry in endothelial cells.
Effect of Tsg101 knockdown on KSHV entry in endothelial cells. Open
(A) HMVEC-d cells were transfected with siControl or siTsg101-RNA and examined after 48 h by Western blotting for Tsg101 and β-Tubulin. (B, C) siControl and siTsg101-RNA transfected HMVEC-d and HUVEC cells were infected with …
View article: Effects of IFI16, H2B, BRCA1, cGAS, STING and ASC knockdown on IFN-β during KSHV and HSV-1 infection.
Effects of IFI16, H2B, BRCA1, cGAS, STING and ASC knockdown on IFN-β during KSHV and HSV-1 infection. Open
(A) WCL from HMVEC-d cells electroporated with siC, siH2B, siIFI16, siSTING or siASC for 48 h followed by infection for 4 h with or without KSHV (KS) were western blotted with anti-IFI16, ASC, H2B and STING antibodies. (B) Phosphorylation …
View article: Association of Tsg101 with other ESCRT complex proteins during KSHV infection.
Association of Tsg101 with other ESCRT complex proteins during KSHV infection. Open
(A) A schematic representation of the ESCRT complex proteins showing what is known about the sequential recruitment of proteins on the endosomal membrane followed by formation of the multi-vesicular body. (B) Serum-starved (8…
View article: CHMP5 colocalizes with Rab5 and Rab7 during <i>de novo</i> KSHV infection in HMVEC-d cells.
CHMP5 colocalizes with Rab5 and Rab7 during <i>de novo</i> KSHV infection in HMVEC-d cells. Open
Serum starved HMVEC-d cells were infected with KSHV (30 DNA copies/cell) for different time points as indicated, washed, and processed for immunofluorescence staining using mouse anti-CHMP5 antibodies. (A, B) Colocalization of CHMP5…
View article: List of antibodies used in this study.
List of antibodies used in this study. Open
View article: List of antibodies used in the study and their source.
List of antibodies used in the study and their source. Open
View article: Demonstration of KSHV latent infection induced IFI16-H2B interaction and redistribution.
Demonstration of KSHV latent infection induced IFI16-H2B interaction and redistribution. Open
(A and B) Cytoplasmic and nuclear extracts from BJAB and BCBL-1 cells were IP-ed using anti-IFI16 and anti-H2B antibodies, and immunoblotted with anti-IFI16, H2B, BRCA1, H2A and ASC antibodies. (C) Cytoplasmic and nuclear fractions were an…
View article: Enhanced interaction of IFI16 with BRCA1 but not with other DNA damage response proteins during <i>de novo</i> KSHV infection.
Enhanced interaction of IFI16 with BRCA1 but not with other DNA damage response proteins during <i>de novo</i> KSHV infection. Open
(A-C) Analysis of de novo infection with BrdU or EdU genome labeled KSHV. (A) HMVEC-d cells were infected with BrdU genome labeled KSHV (30 DNA copies/cell) for 0.25 h and processed for IFA. Cells were fixed, permeabilized, t…
View article: Effect of IFI16 acetylation on IFN-β production during <i>de novo</i> KSHV infection of HMVEC-d cells.
Effect of IFI16 acetylation on IFN-β production during <i>de novo</i> KSHV infection of HMVEC-d cells. Open
HMVEC-d cells in the presence or absence of 1 μM C-646 were either left uninfected or infected with KSHV (30 DNA copies/cell) for 2 h, washed and incubated with or without 1 μM C-646 for 6 h. (A) Interferon-β in the culture supernat…
View article: Effect of p300 inhibitor C-646 on IFI16 and Ran-GTPase association and effect of nuclear export blockage by Leptomycin B and protein synthesis inhibitor cycloheximide on the redistribution of IFI16 during <i>de novo</i> KSHV infection of HMVEC-d cells.
Effect of p300 inhibitor C-646 on IFI16 and Ran-GTPase association and effect of nuclear export blockage by Leptomycin B and protein synthesis inhibitor cycloheximide on the redistribution of IFI16 during <i>de novo</i> KSHV infection of HMVEC-d cells. Open
(A) HMVEC-d cells preincubated with 1 μM C-646 were uninfected or infected with KSHV for 2 h, washed and incubated for 4 h without (UT) or with 1 μM C-646. WCL proteins in NETN lysis buffer were IP-ed with anti-Ran antibodies and im…
View article: PLA analysis demonstrating the role of BRCA1 in HSV-1 genome recognition by IFI16 during <i>de novo</i> infection.
PLA analysis demonstrating the role of BRCA1 in HSV-1 genome recognition by IFI16 during <i>de novo</i> infection. Open
Uninfected HFF cells or HFF cells infected with EdU genome labeled HSV-1 (KOS) with 1 PFU/cell (~25 DNA copies/cell) for 30 min were processed for PLA. (A and D) PLA reaction (green dots) detecting IFI16 (mouse and goat anti-IFI16 a…
View article: Effect of acetylation inhibition by C-646 on the ability of IFI16 to recognize and bind the KSHV genome.
Effect of acetylation inhibition by C-646 on the ability of IFI16 to recognize and bind the KSHV genome. Open
(A and B) HMVEC-d cells pre-incubated with or without 1 μM C-646 for 2 h were washed, infected with BrdU genome labeled KSHV (30 DNA copies/cell) for 2 h, washed and incubated with or without 1 μM C-646 for 4 h. Cells were processed…