K. Michalska
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View article: Structural insights into the inhibition of <i>M. tuberculosis</i> Prolyl-tRNA synthetase by derivatives of 3-aminopyrazine-2-carboxamide
Structural insights into the inhibition of <i>M. tuberculosis</i> Prolyl-tRNA synthetase by derivatives of 3-aminopyrazine-2-carboxamide Open
Tuberculosis (TB) is recognized as the second leading cause of death globally from a single infectious agent, following SARS-CoV-2 pneumonia. Aminoacyl-tRNA synthetases (aaRS) are essential enzymes responsible for attaching amino acids to …
View article: Structural and functional insights into <i>Escherichia coli</i> O32:H37 contact dependent inhibition
Structural and functional insights into <i>Escherichia coli</i> O32:H37 contact dependent inhibition Open
Many gram-negative bacteria utilize contact-dependent growth inhibition (CDI) systems to introduce toxic effector proteins into neighboring cells, thereby outcompeting them. In CDI+ strains, CdiA effector proteins, carrying C-terminal toxi…
View article: Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria
Structural genomics of bacterial drug targets: Application of a high-throughput pipeline to solve 58 protein structures from pathogenic and related bacteria Open
Antibiotic resistance remains a leading cause of severe infections worldwide. Small changes in protein sequence can impact antibiotic efficacy. Here, we report deposition of 58 X-ray crystal structures of bacterial proteins that are known …
View article: Mapping Hsp104 interactions using cross‐linking mass spectrometry
Mapping Hsp104 interactions using cross‐linking mass spectrometry Open
Molecular machines from the AAA+ (ATPases Associated with diverse cellular Activity) superfamily of protein disaggregases play important roles in protein folding, disaggregation and DNA processing. Recent cryo‐EM structures of AAA+ molecul…
View article: Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family
Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family Open
2-Hydroxyacyl-CoA lyase/synthase (HACL/S) is a thiamine diphosphate (ThDP)-dependent versatile enzyme originally discovered in the mammalian α-oxidation pathway. HACL/S natively cleaves 2-hydroxyacyl-CoAs and, in its reverse direction, con…
View article: Revealing reaction intermediates in one-carbon elongation ThDP/CoA dependent enzyme family
Revealing reaction intermediates in one-carbon elongation ThDP/CoA dependent enzyme family Open
2-Hydroxyacyl-CoA lyase/synthase (HACL/S) is a thiamine diphosphate (ThDP)-dependent versatile enzyme originally discovered in the mammalian α-oxidation pathway. HACL/S natively cleaves 2-hydroxyacyl-CoAs and, in its reverse direction, con…
View article: Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human monoclonal antibodies
Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human monoclonal antibodies Open
Coronavirus nucleocapsid protein (NP) of SARS-CoV-2 plays a central role in many functions important for virus proliferation including packaging and protecting genomic RNA. The protein shares sequence, structure, and architecture with nucl…
View article: Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis
Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis Open
Anaerobic fungi found in the guts of large herbivores are prolific biomass degraders whose genomes harbor a wealth of carbohydrate-active enzymes (CAZymes), of which only a handful are structurally or biochemically characterized. Here, we …
View article: Genomes to Structure and Function Workshop Report 2022
Genomes to Structure and Function Workshop Report 2022 Open
The goal of the U.S. Department of Energy (DOE) Biological and Environmental Research (BER) Program is to achieve a predictive understanding of complex biological, earth, and environmental systems with the aim of advancing the nation’s ene…
View article: Fixed-target serial crystallography at the Structural Biology Center
Fixed-target serial crystallography at the Structural Biology Center Open
Serial synchrotron crystallography enables the study of protein structures under physiological temperature and reduced radiation damage by collection of data from thousands of crystals. The Structural Biology Center at Sector 19 of the Adv…
View article: APS-U update and structural biology future directions
APS-U update and structural biology future directions Open
After 25 years in operation, the Advanced Photon Source (APS) is undergoing a massive upgrade.The current electron storage ring will be replaced with state-of-the art technology that will increase the brightness of the X-ray beams up to 50…
View article: Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in <i>Candida albicans</i>
Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in <i>Candida albicans</i> Open
In the human fungal pathogen Candida albicans , ARO1 encodes an essential multi-enzyme that catalyses consecutive steps in the shikimate pathway for biosynthesis of chorismate, a precursor to folate and the aromatic amino acids. We obtaine…
View article: Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin
Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin Open
The Papain-like protease (PLpro) is a domain of a multi-functional, non-structural protein 3 of coronaviruses. PLpro cleaves viral polyproteins and posttranslational conjugates with poly-ubiquitin and protective ISG15, composed of two ubiq…
View article: Fixed-Target Serial Crystallography at Structural Biology Center
Fixed-Target Serial Crystallography at Structural Biology Center Open
Serial synchrotron crystallography enables studies of protein structures under physiological temperature and reduced radiation damage by collection of data from thousands of crystals. The Structural Biology Center at Sector 19 of the Advan…
View article: Target highlights in <scp>CASP14</scp> : Analysis of models by structure providers
Target highlights in <span>CASP14</span> : Analysis of models by structure providers Open
The biological and functional significance of selected Critical Assessment of Techniques for Protein Structure Prediction 14 (CASP14) targets are described by the authors of the structures. The authors highlight the most relevant features …
View article: Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin
Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin Open
The Papain-like protease (PLpro) is a domain of a multi-functional, non-structural protein 3 of coronaviruses. PLpro cleaves viral polyproteins and posttranslational conjugates with poly-ubiquitin and protective ISG15, composed of two ubiq…
View article: Catalytically impaired <scp>TrpA</scp> subunit of tryptophan synthase from <i>Chlamydia trachomatis</i> is an allosteric regulator of <scp>TrpB</scp>
Catalytically impaired <span>TrpA</span> subunit of tryptophan synthase from <i>Chlamydia trachomatis</i> is an allosteric regulator of <span>TrpB</span> Open
Intracellular growth and pathogenesis of Chlamydia species is controlled by the availability of tryptophan, yet the complete biosynthetic pathway for l‐ Trp is absent among members of the genus. Some representatives, however, preserve gene…
View article: 2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography
2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography Open
Significance The 2′-O methyl group in Cap-1 is essential to protect viral RNA from host interferon-induced response. We determined crystal structures of SARS-CoV-2 Nsp10/16 heterodimer in complex with substrates (Cap-0 analog and S-adenosy…
View article: <i>Mycobacterium tuberculosis</i> Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation
<i>Mycobacterium tuberculosis</i> Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation Open
Tuberculosis, caused by Mycobacterium tuberculosis, responsible for ∼1.5 million fatalities in 2018, is the deadliest infectious disease. Global spread of multidrug resistant strains is a public health threat, requiring new treatments. Ami…
View article: Structural genomics and the Protein Data Bank
Structural genomics and the Protein Data Bank Open
The field of Structural Genomics arose over the last 3 decades to address a large and rapidly growing divergence between microbial genomic, functional, and structural data. Several international programs took advantage of the vast genomic …
View article: Therapeutic genetic variation revealed in diverse Hsp104 homologs
Therapeutic genetic variation revealed in diverse Hsp104 homologs Open
The AAA+ protein disaggregase, Hsp104, increases fitness under stress by reversing stress-induced protein aggregation. Natural Hsp104 variants might exist with enhanced, selective activity against neurodegenerative disease substrates. Howe…
View article: Author response: Therapeutic genetic variation revealed in diverse Hsp104 homologs
Author response: Therapeutic genetic variation revealed in diverse Hsp104 homologs Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The AAA+ protein disaggregase, Hsp104, inc…
View article: Structure of Mycobacterium tuberculosis Phe-tRNA synthetase reveals ligand binding and two-stage tRNA recognition
Structure of Mycobacterium tuberculosis Phe-tRNA synthetase reveals ligand binding and two-stage tRNA recognition Open
Tuberculosis, caused by Mycobacterium tuberculosis , responsible for ~1.5 million fatalities in 2018, is the deadliest infectious disease. Global spread of multidrug resistant TB is a public health threat. Aminoacyl-tRNA synthetases are pl…