K. Rollet
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View article: Nucleating Agent Crystallophore Induces Instant Protein Crystallization
Nucleating Agent Crystallophore Induces Instant Protein Crystallization Open
International audience
View article: The nucleating agent crystallophore induces instant protein crystallization
The nucleating agent crystallophore induces instant protein crystallization Open
The rapid preparation of homogeneous suspensions of micro- or nano-crystals is a crucial step in serial crystallography. We show how additives, such as the crystallophore (TbXo4) that acts as a molecular glue by promoting protein-protein i…
View article: Serial crystallography: a game-changer in biomolecule crystallization and crystal handling
Serial crystallography: a game-changer in biomolecule crystallization and crystal handling Open
Over the past decade, the advent of X-ray free electron lasers delivering ultra intense X-ray beams has revolutionized biocrystallography.With a brilliance a billion times higher than at synchrotrons, the XFEL beam destroys the sample just…
View article: From functional study of tRNA CCA-adding enzymes toward their characterization by serial X-ray crystallography
From functional study of tRNA CCA-adding enzymes toward their characterization by serial X-ray crystallography Open
The last decade has seen important developments in the field of structural biology, in particular in time-resolved serial X-ray crystallography (TR-SSX). The objective of this thesis, in cotutelle between the universities of Strasbourg and…
View article: H1153Y-KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating
H1153Y-KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating Open
Long QT syndrome is one of the most common hereditary channelopathies inducing fatal arrhythmias and sudden cardiac death. We identified in a sudden arrhythmic death syndrome case a C-term KCNH2 mutation (c.3457C > T; p.His1153Tyr) classif…
View article: Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip Open
The preparation of well diffracting crystals and their handling before their X-ray analysis are two critical steps of biocrystallographic studies. We describe a versatile microfluidic chip that enables the production of crystals by the eff…
View article: CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus
CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus Open
CCA-adding enzymes are highly specific RNA polymerases that add and maintain the sequence C-C-A at tRNA 3'-ends. Recently, we could reveal that cold adaptation of such a polymerase is not only achieved at the expense of enzyme stability, b…
View article: Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA Substrates
Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA Substrates Open
The mitochondrial genome of the nematode Romanomermis culicivorax encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the consensus cloverleaf. The single tRNA nucleotidyltransferase…
View article: Adaptation of the<i>Romanomermis culicivorax</i>CCA-adding enzyme to miniaturized armless tRNA substrates
Adaptation of the<i>Romanomermis culicivorax</i>CCA-adding enzyme to miniaturized armless tRNA substrates Open
The mitochondrial genome of the nematode Romanomermis culicivorax encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the consensus cloverleaf. The single tRNA nucleotidyltransferase…
View article: Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering
Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering Open
The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called XtalController has recently been designed that allows the monitoring of crystallization a…
View article: Monitoring the production of high diffraction-quality crystals of two enzymes in real time using<i>in situ</i>dynamic light scattering
Monitoring the production of high diffraction-quality crystals of two enzymes in real time using<i>in situ</i>dynamic light scattering Open
The reproducible preparation of well diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called the XtalController has recently been designed that allows the monitoring of crystallizati…
View article: A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography Open
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and the…