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View article: Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis
Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis Open
An essential guanine nucleotide-binding protein, Obg, of Bacillus subtilis has been characterized with respect to its enzymatic activity for GTP. The protein was seen to hydrolyze GTP with a Km of 5.4 microM and a kcat of 0.0061 min-1 at 3…
View article: Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg
Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg Open
The obg gene is part of the spo0B sporulation operon and codes for a GTP-binding protein which is essential for growth. A temperature-sensitive mutant in the obg gene was isolated and found to be the result of two closely linked missense m…
View article: Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppress mutations at several spo0 loci
Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppress mutations at several spo0 loci Open
Secondary site mutations that restore sporulation to sporulation-defective spo0F or spo0B deletion mutants were found to reside in the spo0A gene. Sequence analysis of 23 such sof mutants showed that the sof mutations fell into six classes…
View article: Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis
Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis Open
The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of AT…
View article: The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein
The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein Open
Transcription of the spo0B gene and genes downstream of it was investigated by S1 nuclease protection experiments. The spo0B gene was transcribed from a single promoter, and this transcript extended through a gene, obg, coding for a 47,668…
View article: Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome
Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome Open
The total sequence of a 6,314-base-pair BglII fragment of the Bacillus subtilis chromosome containing the spo0F locus has been accomplished. Several genes of interest have been identified on this DNA fragment. The ctrA locus was recognized…
View article: The primary structure of the mitochondrial energy-linked nicotinamide nucleotide transhydrogenase deduced from the sequence of cDNA clones.
The primary structure of the mitochondrial energy-linked nicotinamide nucleotide transhydrogenase deduced from the sequence of cDNA clones. Open
The amino acid sequence of the bovine mitochondrial nicotinamide nucleotide transhydrogenase, which catalyzes hydride ion transfer between NAD(H) and NADP(H) coupled to proton translocation across the mitochondrial inner membrane, has been…
View article: Structural studies and isolation of cDNA clones providing the complete sequence of rat liver dihydropteridine reductase.
Structural studies and isolation of cDNA clones providing the complete sequence of rat liver dihydropteridine reductase. Open
The cleavage of reductively alkylated rat liver dihydropteridine reductase with cyanogen bromide afforded a mixture of peptides, six of which (CB-1 to CB-6) were isolated and purified by C8 reverse-phase high performance liquid chromatogra…
View article: Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase.
Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase. Open
In previous studies from this laboratory we isolated and characterized a 37-kDa protein that was associated with the membrane of erythroid cells. The polypeptide appeared to undergo a lineage-specific alteration in its interaction with the…
View article: Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. Open
The location of the stage 0 sporulation locus spo0F has been determined on a cloned fragment of Bacillus subtilis DNA. The spo0F gene and surrounding region was sequenced and was shown to code for a protein of Mr 14,286. The amino acid seq…
View article: Characterization of the spo0A locus and its deduced product.
Characterization of the spo0A locus and its deduced product. Open
The highly pleiotropic stage 0 sporulation locus of Bacillus subtilis, spo0A, has been cloned in bacteriophage lambda, subcloned in plasmids, and sequenced. The locus was found to code for a protein of 29,691 Da. Analysis of the in vivo tr…
View article: Sequence analysis of the spo0B locus reveals a polycistronic transcription unit
Sequence analysis of the spo0B locus reveals a polycistronic transcription unit Open
A 2.3-kilobase pair EcoRI fragment containing the spo0B locus has been sequenced. The spo0B locus was shown to code for a protein of 22,542 daltons. Promoter distal to the spo0B locus, an open reading frame was uncovered which was preceded…
View article: Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein
Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein Open
The mutation sof-1 suppresses the sporulation defect of mutations in either the spo0B, spo0E, or spo0F stage 0 sporulation genes. Through the use of integrative plasmids carrying the portion of the chromosome including the spo0A locus and …