Kaiyi Jiang
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View article: Epidemiological investigation of porcine Mycoplasma hyopneumoniae in pig herds in Guangxi, China (2022–2023) and genetic diversity analysis based on multilocus sequence typing
Epidemiological investigation of porcine Mycoplasma hyopneumoniae in pig herds in Guangxi, China (2022–2023) and genetic diversity analysis based on multilocus sequence typing Open
Introduction Mycoplasma hyopneumoniae (Mhp) infection significantly challenges Guangxi’s pig farms, yet its prevalence and molecular characteristics remain poorly understood. This study aimed to define circulating Mhp genotypes and their d…
View article: Fanzors, a family of eukaryotic <scp>RNA</scp>‐guided <scp>DNA</scp> endonucleases
Fanzors, a family of eukaryotic <span>RNA</span>‐guided <span>DNA</span> endonucleases Open
Fanzor proteins represent the first confirmed family of RNA‐guided DNA endonucleases in eukaryotes. Originally identified in 2013 as TnpB homologs, Fanzors remained functionally uncharacterized until 2023, when three independent research g…
View article: Rapid protein evolution by few-shot learning with a protein language model
Rapid protein evolution by few-shot learning with a protein language model Open
Directed evolution of proteins is critical for applications in basic biological research, therapeutics, diagnostics, and sustainability. However, directed evolution methods are labor intensive, cannot efficiently optimize over multiple pro…
View article: Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses
Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses Open
Programmable RNA-guided DNA nucleases perform numerous roles in prokaryotes, but the extent of their spread outside prokaryotes is unclear. Fanzors, the eukaryotic homolog of prokaryotic TnpB proteins, have been detected in genomes of euka…
View article: Engineering synthetic phosphorylation signaling networks in human cells
Engineering synthetic phosphorylation signaling networks in human cells Open
Protein phosphorylation signaling networks play a central role in how cells sense and respond to their environment. Here, we describe the engineering of artificial phosphorylation networks in which “push-pull” motifs—reversible enzymatic p…
View article: Programmable RNA-guided endonucleases are widespread in eukaryotes and their viruses
Programmable RNA-guided endonucleases are widespread in eukaryotes and their viruses Open
TnpB proteins are RNA-guided nucleases that are broadly associated with IS200/605 family transposons in prokaryotes. TnpB homologs, named Fanzors, have been detected in genomes of some eukaryotes and large viruses, but their activity and f…
View article: RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease
RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease Open
The type III-E CRISPR–Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo–electron microscopy structures of the Cas7-11–crRNA–Csx29 complex with and without ta…
View article: RNA-triggered protein cleavage and cell death by the RNA-guided type III-E CRISPR-Cas nuclease-protease complex
RNA-triggered protein cleavage and cell death by the RNA-guided type III-E CRISPR-Cas nuclease-protease complex Open
The type III-E Cas7-11 effector nuclease forms a complex with a CRISPR RNA (crRNA) and the putative caspase-like protease Csx29, catalyzes crRNA-guided target RNA cleavage, and has been used for RNA targeting in eukaryotic cells. Here, we …
View article: Programmable eukaryotic protein expression with RNA sensors
Programmable eukaryotic protein expression with RNA sensors Open
The diversity of cell types and states can be scalably measured and defined by expressed RNA transcripts. However, approaches to programmably sense and respond to the presence of specific RNAs within living biological systems with high sen…
View article: Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases
Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases Open
Programmable and multiplexed genome integration of large, diverse DNA cargo independent of DNA repair remains an unsolved challenge of genome editing. Current gene integration approaches require double-strand breaks that evoke DNA damage r…
View article: Sub-second multi-channel magnetic control of select neural circuits in behaving flies
Sub-second multi-channel magnetic control of select neural circuits in behaving flies Open
Precisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or “magnetogenetics” using magnetic nanoparticle heating of te…
View article: Sub-second multi-channel magnetic control of select neural circuits in behaving flies
Sub-second multi-channel magnetic control of select neural circuits in behaving flies Open
Precisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or “magnetogenetics” using magnetic nanoparticle heating of te…
View article: Designing Iron Oxide Nanoparticles for Image Guided Thermal Medicine Applications
Designing Iron Oxide Nanoparticles for Image Guided Thermal Medicine Applications Open
This work evaluates MRI relaxation and the specific absorption rate properties of iron oxide nanoparticles (IONPs) as a function of diameter (6-32 nm). We conclude that the ideal IONP diameter for image guided heating applications is depen…