Kangseok Lee
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View article: CRISPR-Cas technologies: Emerging tools from research to clinical application
CRISPR-Cas technologies: Emerging tools from research to clinical application Open
CRISPR-Cas technologies have emerged as powerful and versatile tools in gene therapy. In addition to the widely used SpCas9 system, alternative platforms including modified amino acid sequences, size-optimized variants, and other Cas enzym…
View article: Genetic “expiry-date” circuits control lifespan of synthetic scavenger bacteria for safe bioremediation
Genetic “expiry-date” circuits control lifespan of synthetic scavenger bacteria for safe bioremediation Open
Synthetic biology enabled the systematic engineering of bacteria for diverse applications, but their deployment in open environments raises concerns about their persistence and unintended ecological impacts. To address these challenges, ge…
View article: Autoinhibited Protein Database: a curated database of autoinhibitory domains and their autoinhibition mechanisms
Autoinhibited Protein Database: a curated database of autoinhibitory domains and their autoinhibition mechanisms Open
Autoinhibition, a crucial allosteric self-regulation mechanism in cell signaling, ensures signal propagation exclusively in the presence of specific molecular inputs. The heightened focus on autoinhibited proteins stems from their implicat…
View article: Sua5 catalyzing universal t <sup>6</sup> A tRNA modification is responsible for multifaceted functions of the KEOPS complex in <i>Cryptococcus neoformans</i>
Sua5 catalyzing universal t <sup>6</sup> A tRNA modification is responsible for multifaceted functions of the KEOPS complex in <i>Cryptococcus neoformans</i> Open
The N 6 -threonylcarbamoyl adenosine (t 6 A) tRNA modification is critical for ensuring translation fidelity across three domains of life. Our prior work highlighted the KEOPS complex, organized in a Pcc1-Kae1-Bud32-Cgi121 linear arrangeme…
View article: Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species Open
Heterogeneity of ribosomal RNA (rRNA) sequences has recently emerged as a mechanism that can lead to subpopulations of specialized ribosomes. Our previous study showed that ribosomes containing highly divergent rRNAs expressed from the rrn…
View article: A multi‐layered network model identifies Akt1 as a common modulator of neurodegeneration
A multi‐layered network model identifies Akt1 as a common modulator of neurodegeneration Open
The accumulation of misfolded and aggregated proteins is a hallmark of neurodegenerative proteinopathies. Although multiple genetic loci have been associated with specific neurodegenerative diseases (NDs), molecular mechanisms that may hav…
View article: KPNA3 promotes epithelial-mesenchymal transition by regulating TGF-β and AKT signaling pathways in MDA-MB-231, a triple-negative breast cancer cell line
KPNA3 promotes epithelial-mesenchymal transition by regulating TGF-β and AKT signaling pathways in MDA-MB-231, a triple-negative breast cancer cell line Open
Karyopherin-α3 (KPNA3), a karyopherin- α isoform, is intimately associated with metastatic progression via epithelial-mesenchymal transition (EMT). However, the molecular mechanism underlying how KPNA3 acts as an EMT inducer remains to be …
View article: Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency
Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency Open
Genome editing using CRISPR-associated technology is widely used to modify the genomes rapidly and efficiently on specific DNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering, st…
View article: Unraveling the Pathobiological Role of the Fungal KEOPS Complex in Cryptococcus neoformans
Unraveling the Pathobiological Role of the Fungal KEOPS Complex in Cryptococcus neoformans Open
The cellular function and structural configuration of the KEOPS complex have been elucidated in some eukaryotes and archaea but have never been fully characterized in fungal pathogens. Here, we comprehensively analyzed the pathobiological …
View article: Specialised ribosomes as versatile regulators of gene expression
Specialised ribosomes as versatile regulators of gene expression Open
The ribosome has long been thought to be a homogeneous cellular machine that constitutively and globally synthesises proteins from mRNA. However, recent studies have revealed that ribosomes are highly heterogeneous, dynamic macromolecular …
View article: 5′-tRNAGly(GCC) halves generated by IRE1α are linked to ER stress response
5′-tRNAGly(GCC) halves generated by IRE1α are linked to ER stress response Open
Transfer RNA (tRNA) halves (tRHs) have various biological functions. However, the biogenesis of specific 5′-tRHs under certain conditions remains unknown. Here, we report that inositol-requiring enzyme 1α (IRE1α) cleaves the anticodon stem…
View article: The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation
The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation Open
The synaptonemal complex (SC) is a proteinaceous structure that mediates homolog engagement and genetic recombination during meiosis. In budding yeast, Zip-Mer-Msh (ZMM) proteins promote crossover (CO) formation and initiate SC formation. …
View article: Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression
Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression Open
Bacteria utilize endoribonuclease-mediated RNA processing and decay to rapidly adapt to environmental changes. Here, we report that the modulation of hns mRNA stability by the endoribonuclease RNase G plays a key role in Salmonella Typhimu…
View article: Substrate-dependent effects of quaternary structure on RNase E activity
Substrate-dependent effects of quaternary structure on RNase E activity Open
RNase E is an essential, multifunctional ribonuclease encoded in E. coli by the rne gene. Structural analysis indicates that the ribonucleolytic activity of this enzyme is conferred by rne -encoded polypeptide chains that (1) dimerize to f…
View article: The Synaptonemal Complex Central Region Modulates Crossover Pathways and Feedback Control of Meiotic Double-strand Break Formation
The Synaptonemal Complex Central Region Modulates Crossover Pathways and Feedback Control of Meiotic Double-strand Break Formation Open
Summary The synaptonemal complex (SC) is a proteinaceous structure that mediates homolog engagement and genetic recombination during meiosis. Zip-Mer-Msh (ZMM) proteins promote crossover (CO) formation and initiate SC formation. In SC elon…
View article: An alternative miRISC targeting a coding mutation site in<i>FOXL2</i>links to granulosa cell tumor
An alternative miRISC targeting a coding mutation site in<i>FOXL2</i>links to granulosa cell tumor Open
Recent evidence suggests that animal microRNAs (miRNAs) can target coding sequences (CDSs); however, the pathophysiological importance of such targeting remains unknown. Here, we show that a somatic heterozygous missense mutation (c.402C>G…
View article: Comparative Research on the Importance and Performance of Instructor Pilots’ Core Competencies
Comparative Research on the Importance and Performance of Instructor Pilots’ Core Competencies Open
Transportation, which was mostly carried out by land transportation in the past, is moving to the sky in large part due to recent technological developments in aviation.The passenger transportation revenue of Korean airlines has increased …
View article: The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli Open
Rapid modulation of RNA function by endoribonucleases during physiological responses to environmental changes is known to be an effective bacterial biochemical adaptation. We report a molecular mechanism underlying the regulation of enolas…
View article: The bacterial endoribonuclease RNase E can cleave RNA in the absence of the RNA chaperone Hfq
The bacterial endoribonuclease RNase E can cleave RNA in the absence of the RNA chaperone Hfq Open
RNase E is a component of the RNA degradosome complex and plays a key role in RNA degradation and maturation in Escherichia coli RNase E-mediated target RNA degradation typically involves the RNA chaperone Hfq and requires small guide RNAs…
View article: Correction: Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus
Correction: Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus Open
[This corrects the article DOI: 10.1371/journal.pone.0190064.].
View article: Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus
Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus Open
RNase E has a pivotal role in the degradation and processing of RNAs in Escherichia coli, and protein inhibitors RraA and RraB control its enzymatic activity. The halophilic pathogenic bacterium Vibrio vulnificus also expresses orthologs o…
View article: Antimicrobial peptide-loaded gold nanoparticle-DNA aptamer conjugates as highly effective antibacterial therapeutics against Vibrio vulnificus
Antimicrobial peptide-loaded gold nanoparticle-DNA aptamer conjugates as highly effective antibacterial therapeutics against Vibrio vulnificus Open
Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against V. vulnificus infection. However, the therapeutic effects of antibiotics are limited by multidrug resistance. In this stud…
View article: Implications of Streptomyces coelicolor RraAS1 as an activator of ribonuclease activity of Escherichia coli RNase E
Implications of Streptomyces coelicolor RraAS1 as an activator of ribonuclease activity of Escherichia coli RNase E Open
RNase E는 대장균(Escherichia coli)에서 수많은 RNA의 가공 및 분해에 관여하는 필수적인 효소이다. RNase E의 효소 활성은 RraA와 RraB에 의해 조절된다. 그람양성균인 Streptomyces coelicolor는 RNase ES, RraAS1, RraAS2라고 명명되는 RNase E와 RraA의 동족체를 가지고 있다. 이 연구에서는 S. coelicolor 유래의 RraAS1이 E. coli에서…