Karel Melzoch
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View article: Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins
Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins Open
Folding and processing of proteins in the endoplasmic reticulum (ER) are major impediments in the production and secretion of proteins from Pichia pastoris ( Komagataella sp.). Overexpression of recombinant genes can overwhelm the innate s…
View article: Comparison of Lactic Acid Production by L. casei in Batch, Fed-batch and Continuous Cultivation, Testing the use of Feather Hydrolysate as a Complex Nitrogen Source
Comparison of Lactic Acid Production by L. casei in Batch, Fed-batch and Continuous Cultivation, Testing the use of Feather Hydrolysate as a Complex Nitrogen Source Open
A comprehensive comparison of the main fermentation parameters, productivity, yield and final L-lactic acid concentration, obtained through batch, fed-batch and continuous cultivations using Lactobacillus casei CCDM 198 and a model cultiva…
View article: Optimization of feeding strategy for the ergosterol production by yeasts saccharomyces cerevisiae
Optimization of feeding strategy for the ergosterol production by yeasts saccharomyces cerevisiae Open
Objective of this study was to optimize ergosterol production by yeast strain Saccharomyces cerevisiae with the use of computer controlled feeding of cultivation medium. Baker´s yeasts strain of Saccharomyces cerevisiae originally modified…
View article: Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris
Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris Open
Pichia pastoris (Komagataella sp.) is broadly used for the production of secreted recombinant proteins. Due to the high rate of protein production, incorrectly folded proteins may accumulate in the endoplasmic reticulum (ER).…
View article: Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production
Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production Open
Biotechnological production of lactic acid has experienced a boom that is hindered only by the lack of low-cost, abundant material that might be used as a substrate for lactic acid bacteria. Such material should contain not only carbon but…
View article: Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter Open
As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates, it is a desirable production system. The function of a novel synthetic variant of the AOX1 promoter was characterised comprehensively usi…
View article: Cassava roots: perspectives of a traditional staple for bio-solvents production
Cassava roots: perspectives of a traditional staple for bio-solvents production Open
The culture media prepared from mashed cassava and cassava starch were used to test their convertibility into organic solvents such as acetone, butanol, and ethanol. The trials were conducted in Erlenmeyer flasks containing various culture…
View article: Changes in Membrane Plasmalogens of Clostridium pasteurianum during Butanol Fermentation as Determined by Lipidomic Analysis
Changes in Membrane Plasmalogens of Clostridium pasteurianum during Butanol Fermentation as Determined by Lipidomic Analysis Open
Changes in membrane lipid composition of Clostridium pasteurianum NRRL B-598 were studied during butanol fermentation by lipidomic analysis, performed by high resolution electrospray ionization tandem mass spectrometry. The highest content…