Karsten Eichholz
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View article: Anti–PD-1 chimeric antigen receptor T cells efficiently target SIV-infected CD4+ T cells in germinal centers
Anti–PD-1 chimeric antigen receptor T cells efficiently target SIV-infected CD4+ T cells in germinal centers Open
Programmed cell death protein 1 (PD-1) is an immune checkpoint marker commonly expressed on memory T cells and enriched in latently HIV-infected CD4+ T cells. We engineered an anti-PD-1 chimeric antigen receptor (CAR) to assess the impact …
View article: Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement Open
This study examines the interactions between danger-associated molecular patterns and human adenoviruses, and their impact on vaccines. HAdVs and HNP-1 can interact, and these interactions will modify the response of antigen-presenting cel…
View article: Adenovirus-α-defensin complexes induce NLRP3-associated maturation of human phagocytes via TLR4 engagement
Adenovirus-α-defensin complexes induce NLRP3-associated maturation of human phagocytes via TLR4 engagement Open
Intramuscular delivery of human adenovirus (HAdV)-based vaccines leads to rapid recruitment of neutrophils, which then release antimicrobial peptides/proteins (AMPs). How these AMPs influence vaccine efficacy over the subsequent 24 hr is p…
View article: Immune inactivation of anti-simian immunodeficiency virus chimeric antigen receptor T cells in rhesus macaques
Immune inactivation of anti-simian immunodeficiency virus chimeric antigen receptor T cells in rhesus macaques Open
Chimeric antigen receptor (CAR) T cell therapies are being investigated as potential HIV cures and designed to target HIV reservoirs. Monoclonal antibodies (mAbs) targeting the simian immunodeficiency virus (SIV) envelope allowed us to inv…
View article: Lactoferrin Retargets Human Adenoviruses to TLR4 to Induce an Abortive NLRP3-Associated Pyroptotic Response in Human Phagocytes
Lactoferrin Retargets Human Adenoviruses to TLR4 to Induce an Abortive NLRP3-Associated Pyroptotic Response in Human Phagocytes Open
Despite decades of clinical and preclinical investigations, we still poorly grasp our innate immune response to human adenoviruses (HAdVs) and their vectors. In this study, we explored the impact of lactoferrin on three HAdV types that are…
View article: CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice
CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice Open
Chronic hepatitis B virus (HBV) infection is a major public health problem. New treatment approaches are needed because current treatments do not target covalently closed circular DNA (cccDNA), the template for HBV replication, and rarely …
View article: RRScell method for automated single-cell profiling of multiplexed immunofluorescence cancer tissue
RRScell method for automated single-cell profiling of multiplexed immunofluorescence cancer tissue Open
Multiplexed immuno-fluorescence tissue imaging, allowing simultaneous detection of molecular properties of cells, is an essential tool for characterizing the complex cellular mechanisms in translational research and clinical practice. New …
View article: Real-Time Killing Assays to Assess the Potency of a New Anti-Simian Immunodeficiency Virus Chimeric Antigen Receptor T Cell
Real-Time Killing Assays to Assess the Potency of a New Anti-Simian Immunodeficiency Virus Chimeric Antigen Receptor T Cell Open
The success of chimeric antigen receptor (CAR) T cell therapies for treating leukemia has resulted in a booming interest for the technology. Expression of a CAR in T cells allows redirection of their natural cytolytic activity toward cells…
View article: A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue
A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue Open
Chimeric antigen receptor (CAR) T cells are engineered cells used in cancer therapy and are studied to treat infectious diseases. Trafficking and persistence of CAR T cells is an important requirement for efficacy to target cancer. Here, w…
View article: Lactoferrin retargets adenoviruses to TLR4 to induce an abortive NLRP3-associated pyroptotic response in human dendritic cells
Lactoferrin retargets adenoviruses to TLR4 to induce an abortive NLRP3-associated pyroptotic response in human dendritic cells Open
Despite decades of investigations, we still poorly grasp the immunogenicity of human adenovirus (HAdV)-based vaccines in humans. In this study, we explored the role of lactoferrin, which belong to the alarmin subset of antimicrobial peptid…
View article: A review of 65 years of human adenovirus seroprevalence
A review of 65 years of human adenovirus seroprevalence Open
Introduction: Human adenovirus (HAdV)-derived vectors have been used in numerous pre-clinical and clinical trials during the last 40 years. Current research in HAdV-based vaccines focuses on improving transgene immunogenicity and safety. B…
View article: A review of 65 years of human adenovirus seroprevalence
A review of 65 years of human adenovirus seroprevalence Open
Introduction: Human adenovirus (HAdV)-derived vectors have been used in numerous pre-clinical and clinical trials during the last 40 years. Current research in HAdV-based vaccines focuses on improving transgene immunogenicity and safety. B…
View article: A review of 65 years of human adenovirus seroprevalence
A review of 65 years of human adenovirus seroprevalence Open
Introduction: Human adenovirus (HAdV)-derived vectors have been used in numerous pre-clinical and clinical trials during the last 40 years. Current research in HAdV-based vaccines focuses on improving transgene immunogenicity and safety. B…
View article: Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells
Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells Open
Following repeated encounters with adenoviruses most of us develop robust humoral and cellular immune responses that are thought to act together to combat ongoing and subsequent infections. Yet in spite of robust immune responses, adenovir…
View article: Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells
Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells Open
Following repeated encounters with adenoviruses most of us develop robust humoral and cellular immune responses that are thought to act together to combat ongoing and subsequent infections. Yet in spite of robust immune responses, adenovir…
View article: IC-HAdVs induce AIM2-associated pyroptosomes.
IC-HAdVs induce AIM2-associated pyroptosomes. Open
Involvement of AIM2 and/or TLR9 in IC-HAdV-induced inflammatory cell death was assessed by RT-qPCR, RNAi, pharmacological inhibitors, flow cytometry, fluorescence microscopy and ELISA. A) Lentiviral-mediated shRNA knockdown of AIM2 in MoDC…
View article: IC-HAdV induce MoDC maturation through DNA sensors TLR9.
IC-HAdV induce MoDC maturation through DNA sensors TLR9. Open
TNF secretion in response to IC-HAdV was measured in MoDCs after lentivirus-mediated shRNA knockdown of TLR9, MyD88 and AP3B1. A) Immunoblotting demonstrating lentivirus-mediated shRNA knockdown of the TLR9 pathway in MoDC. MoDCs were expo…
View article: IC-HAdV induce membrane damage and gain access to the cytosol.
IC-HAdV induce membrane damage and gain access to the cytosol. Open
MoDCs were exposed to Alexa488-HAdV-C5, IC-HAd-488 or IVIg for 3 h and processed for confocal immunofluorescence analysis and stained with anti-p62 (n = 3) A) and galectin-3 (n = 3) B). Scale bar = 5 μm. C) Transmission electron microscopy…
View article: Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells
Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells Open
Human adenoviruses (HAdVs) are nonenveloped proteinaceous particles containing a linear double-stranded DNA genome. HAdVs cause a spectrum of pathologies in all populations regardless of health standards. Following repeat exposure to multi…